Protective effects of human saliva on experimental murine oral candidiasis

Kamagata‐Kiyoura, Yusuke; Abe, Shigeru; Yamaguchi, Hideyo; Nitta, Toshimasa

doi:10.1007/s10156-004-0330-6

Cited by 20 publications

(12 citation statements)

References 13 publications

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“…The authors also described the appearance of lesions consisting of white patches on the tongue dorsum. This methodology has been used by many others authors to analyze the development of disease during their experiments 41 , 43 , 62 , 63 , 67 , 68 …”

Section: Methods For Analysisof Experimental Oral Candidiasismentioning

confidence: 99%

“…The advantage of this model is the development of thrush-type oral candidiasis that mimics the natural infection in humans and is useful for both symptomatological and mycological evaluation of the responsiveness to antifungal treatments. Therefore, several treatments for oral candidiasis were tested in this model including: salivary factors, 62 , 63 quorum-sensing molecules, 64 photodynamic therapy, 43 , 66 natural plant extracts, 41 , 67 and inhibitors of efflux pump 68 …”

Section: Models Of Experimental Oral Candidiasis In Micementioning

confidence: 99%

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Recent mouse and rat methods for the study of experimental oral candidiasis

et al. 2013

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The Candida genus expresses virulence factors that, when combined with immunosuppression and other risk factors, can cause different manifestations of oral candidiasis. The treatment of mucosal infections caused by Candida and the elucidation of the disease process have proven challenging. Therefore, the study of experimentally induced oral candidiasis in rats and mice is useful to clarify the etiopathology of this condition, improve diagnosis, and search for new therapeutic options because the disease process in these animals is similar to that of human candidiasis lesions. Here, we describe and discuss new studies involving rat and mouse models of oral candidiasis with respect to methods for inducing experimental infection, methods for evaluating the development of experimental candidiasis, and new treatment strategies for oral candidiasis.

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Section: Methods For Analysisof Experimental Oral Candidiasismentioning

confidence: 99%

Section: Models Of Experimental Oral Candidiasis In Micementioning

confidence: 99%

Recent mouse and rat methods for the study of experimental oral candidiasis

et al. 2013

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“…As reported previously, some of the essential oils, 7,8) fatty acids 9) or other substances 10,11) are therapeutically efficacious to murine oral candidiasis. In preceding papers, we have reported that oral treatment with tea tree oil (TTO) and its main component, terpinen-4-ol (T-4-ol), improved Candida infected lesions on the tongue's surface and lowered the number of Candida cells in the murine oral cavity.…”

mentioning

confidence: 90%

Suppression of Inflammatory Reactions by Terpinen-4-ol, a Main Constituent of Tea Tree Oil, in a Murine Model of Oral Candidiasis and Its Suppressive Activity to Cytokine Production of Macrophages <i>in Vitro</i>

Ninomiya

Hayama

Ishijima

et al. 2013

Biological & Pharmaceutical Bulletin

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The onset of oral candidiasis is accompanied by inflammatory symptoms such as pain in the tongue, edema or tissue damage and lowers the quality of life (QOL) of the patient. In a murine oral candidiasis model, the effects were studied of terpinen-4-ol (T-4-ol), one of the main constituents of tea tree oil, Melaleuca alternifolia, on inflammatory reactions. When immunosuppressed mice were orally infected with Candida albicans, their tongues showed inflammatory symptoms within 24 h after the infection, which was monitored by an increase of myeloperoxidase activity and macrophage inflammatory protein-2 in their tongue homogenates. Oral treatment with 50 µL of 40 mg/mL terpinen-4-ol 3h after the Candida infection clearly suppressed the increase of these inflammatory parameters. In vitro analysis of the effects of terpinen-4-ol on cytokine secretion of macrophages indicated that 800 µg/mL of this substance significantly inhibited the cytokine production of the macrophages cultured in the presence of heat-killed C. albicans cells. Based on these findings, the role of the anti-inflammatory action of T-4-ol in its therapeutic activity against oral candidiasis was discussed.

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“…23 In ill neonates, oral candidiasis may be a step in the pathogenesis of systemic candidiasis, a condition with a high mortality rate. 24 A Cochrane Review by Austin and Darlow 25 indicates that there is insufficient evidence to support the use of prophylactic oral antifungal agents in very low birth weight infants to reduce oral candida.…”

Section: Discussionmentioning

confidence: 99%

ETCare: a randomized, controlled, masked trial comparing two solutions for upper airway care in the NICU

et al. 2007

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Objective: Small quantities of normal saline are sometimes instilled into the endotracheal tube of intubated neonates, to assist with the removal of thick secretions and maintain patency of the endotracheal tube. However, saline is detrimental to the innate immune system of the upper airway mucosa, rapidly unfolding and inactivating antimicrobial peptides such as LL-37. We previously reported the preparation and feasibility testing of 'ETCare', a low-sodium, physiologically based solution for airway care, and we now report results of a randomized, masked, controlled, two-centered study testing ETCare vs sterile saline among 60 intubated NICU patients.Study Design: Sixty intubated NICU patients were randomized to having their airway care with ETCare vs saline. Three hypotheses were tested: (1) tolerance -patients will tolerate ETCare for airway care as well as they tolerate saline, (2) nosocomial infections -ETCare will result in fewer tracheal aspirates where organisms grow and fewer cases of nosocomial sepsis, and (3) chronic lung disuse -ETCare will result in fewer patients discharged home on supplemental O 2 .Result: Thirty NICU patients with an endotracheal tube in place were randomized to receive their airway care with ETCare, and 30 to receive their care with saline. Only the pharmacist was aware of the randomization; the two solutions were visually indistinguishable and were dispensed in identical syringes. Tolerance of the solutions was similar. The ETCare recipients had trends toward fewer positive blood cultures (odds ratios (OR), 0.48; 95% confidence interval (CI), 0.13 to 1.68), and fewer discharges home on supplemental O 2 (OR, 0.43; 95% CI, 0.14 to 1.32; P ¼ 0.075). Conclusion:On the basis of this study and our previous 10-patient feasibility trial, we maintain that, for airway care, intubated NICU patients tolerate ETCare as well as saline. Data from this study can be used in estimating the sample sizes needed for a phase III trial. We speculate that such a trial will demonstrate that, compared with saline, ETCare will result in fewer nosocomial infections and less chronic lung disease.

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Protective effects of human saliva on experimental murine oral candidiasis

Cited by 20 publications

References 13 publications

Recent mouse and rat methods for the study of experimental oral candidiasis

Recent mouse and rat methods for the study of experimental oral candidiasis

Suppression of Inflammatory Reactions by Terpinen-4-ol, a Main Constituent of Tea Tree Oil, in a Murine Model of Oral Candidiasis and Its Suppressive Activity to Cytokine Production of Macrophages <i>in Vitro</i>

ETCare: a randomized, controlled, masked trial comparing two solutions for upper airway care in the NICU

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