Protective Effects of Ligularia fischeri and Aronia melanocarpa Extracts on Alcoholic Liver Disease (In Vitro and In Vivo Study)

Abstract

Hepatic protective effects of Ligularia fischeri (LF) and Aronia melanocarpa (AM) against alcohol were investigated in vitro and in vivo test. LF, AM, and those composed mixing material (LF+AM) were treated in HepG2 cell. Alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities were significantly increased in each singleness extract and mixed composite. The protective effect on alcoholic liver damage was investigated by animal models. Serum alcohol level and acetaldehyde level were significantly decreased by LF+AM treatment in acute experimental model. In the chronic mouse model study, we had found that the increased plasma liver damage index (alkaline phosphatase) by alcohol treatment was declined by oral administration of LF+AM extraction composite. As well as, it was identified that the protection effect was induced by increasing catalase activity and suppressing COX-2, TNF-α, MCP-1, and IL-6 mRNA expressions. CYP2E1 mRNA expression was also increased. These results suggest that oral ingestion of LF and AM mixed composite is able to protect liver against alcohol-induced injury by increasing alcohol metabolism activity and antioxidant system along with decreasing inflammatory responses.

Metformin and Probiotics Interplay in Amelioration of Ethanol-Induced Oxidative Stress and Inflammatory Response in an In Vitro and In Vivo Model of Hepatic Injury

“…The lipid-containing bottom solutions were collected, and then, pellets were obtained by evaporating chloroform via centrifugation for 2 h using a centrifugal vacuum concentrator. Later, lipids were solubilized using chloroform, and then, hepatic triglyceride and total cholesterol were mea-sured using a quantitative measurement kit and followed according to the kit manufacturer's protocol [29].…”

TRPV6 channel mediates alcohol-induced gut barrier dysfunction and systemic response

“…These concentrations of EtOH and AA are physiologically relevant ( Ferrier et al, 2006 ; Guillot et al, 2019 ). Plasma AA concentrations vary from 40 to 200 μM after chronic alcohol feeding ( Gao et al, 2019 ; Guillot et al, 2019 ; Hao et al, 2018 ; Shimoda et al, 2016 ), whereas 400–1,000 μM AA ( Han et al, 2019 ; Pyun et al, 2020 ) were recorded after acute alcohol administration. SOR-C13, a short peptide based on the amino acid sequence of the protein soricidin, is a specific inhibitor of TRPV6 ( Bowen et al, 2013 ).…”

Efficacy and Safety of Aronia, Red Ginseng, Shiitake Mushroom, and Nattokinase Mixture on Insulin Resistance in Prediabetic Adults: A Randomized, Double-Blinded, Placebo-Controlled Trial

“…It is also related to elevated serum levels of CRP, tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 [ 44 ]. Previous studies have demonstrated that Aronia and ginseng improve insulin resistance and NAFLD and reduce oxidative stress, inflammation [ 45 ], and alcohol-induced liver damage [ 46 ]. In the present study, AGM intake reduced the serum activities of γ-GT and ALT and serum CRP concentrations, which suggests AGM can reduce hepatic insulin resistance and inflammation.…”