Protective effects ofStachys sieboldiiMIQ extract in SK-N-SH cells and its memory ameliorative effect in mice

Abstract

This work aims to evaluate the potential effect of Stachys sieboldii MIQ (SS) against oxidative stress induced by hydrogen peroxide (H2O2) in SK‐N‐SH cells and memory enhancement in ICR mice using the passive avoidance test. The effect of SS in cell proliferation was measured by BrdU incorporation assay with dosages of SS from 0 to 2 mg/mL. SS partially antagonized the effect of 150 μM H2O2 on cell proliferation. In vivo study, SS (100, 250, and 500 mg/kg) was administered orally to mice for 4 weeks. SS led to a significantly longer step‐through latency time compared to control mice. Memory enhancing effect of SS (500 mg/kg) was also observed in amnesia model, SS escalate the fear memory (p < .05), increase acetylcholine and choline acetyl transferase level in hippocampus and cortex than nontreated group. These results suggest that SS can be used to manage memory related disorders in animals. Practical applications Acetylcholine is a cholinergic neurotransmitter known for its various medicinal properties, reducing dementia, depression, cancer, inflammation, and is also required to build muscles in the human body. Hence, the development of a choline‐rich source is of great interest. SS is a good source for vitamins especially vitamin B complex, which is a major precursor for choline synthesis in human. Therefore, this study investigates the memory enhancement effects of Stachys sieboldii MIQ (SS), a choline‐rich plant, in SK‐N‐SH cells and an in vivo animal model. SK‐N‐SH cells and mice treated with SS exhibited memory ameliorative effects. This study sheds light on using plant‐based products as potential functional foods and nutraceuticals for cognition improvement. The results of this study could serve as a foundation for further research on SS extract as a source of choline and promotes the consumption of Stachys sieboldii on a large scale.

Stachys sieboldii Extract Supplementation Attenuates Memory Deficits by Modulating BDNF-CREB and Its Downstream Molecules, in Animal Models of Memory Impairment

“…In agreement, we have previously reported protective effects of Stachys sieboldii MIQ (SS) against H 2 O 2 induced cytotoxicity in SK-N-SH cells (Human neuroblastoma cell line) and memory amelioration in mice [ 22 ]. Notably, an underlying molecular mechanism by which SS exert the neuroprotective effect has not yet been studied.…”

“…Alteration of cholinergic neurons in the hippocampus and frontal cortex are implicated in the cognitive disorders [ 3 ]. Acetylcholine synthesized by choline acetyl transferase in neurons and hydrolyzed by acetylcholine transferase after its release, is essential to memory process [ 22 ]. Therapies designed to reverse the cholinergic deficit in large measure based on their importance in cognition.…”

“…They are also a good source of oligosaccharides, proteins, and water-soluble vitamins (vitamin B complex), which are all major contributors to human nutrition [ 21 ]. Several reports have detailed the health benefits of Stachys sieboldii , such as its anti-inflammatory activity, ability to lower anoxia, immunosuppressive function, antinephritic activity, antioxidant, antimicrobial activity, they also improve memory and discomfort [ 20 , 21 , 22 , 23 ].…”

Antiobesity Effects of Purple Perilla (Perilla frutescens var. acuta) on Adipocyte Differentiation and Mice Fed a High‐fat Diet

“…TG and TC from liver tissue were analyzed using a previously described method (Vijaya Abinaya et al, 2017). Tissue was homogenized using chloroform: methanol solution (2:1) was vortexed and centrifuged.…”

“…There has been a rapid global increase in the use of herbs to treat various health problems over the past few decades (Ekor, 2013;Pichiah & Cha, 2015; Vijaya Abinaya, Kim, Lee, & Cha, 2017). Perilla frutescens, an edible plant with medicinal properties, belongs to the mint family Lamiaceae.…”