Protective Effects of Inflammation of Curcumae Longae Rhizoma 30% EtOH Extract on Acute Reflux Esophagitis Rats

Lee, Jin A; Shin, Mi‐Rae; Kim, Min Ju; Lee, Ji Hye; Park, Hae-Jin; Roh, Seong‐Soo

doi:10.1155/2021/8854945

Cited by 7 publications

(7 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the study, we identified the downregulation of claudin-5 expression in damaged esophageal mucosal, while the expression of claudin-5 was increased by treatments of the ESE and ranitidine. Reflux esophagitis reportedly correlates with changes in the level of tight junction and inflammatory proteins, such as claudin, COX-2 and TNF-α (Nan, 2018;Hatware, 2018;Nam, 2019;Tetreault, 2016, Roh, 2021. In this study, the increase in COX-2 and TNF-α levels was confirmed in the reflux esophagitis model, and a significant decrease in inflammatory cytokine and protein was observed in the group treated with the ESE 100 mg/kg and the ranitidine group.…”

Section: Discussionsupporting

confidence: 63%

Functional Properties of the Water Extract from Eriocheir sinensis (Chinese mitten crab) Via Evaluation of Protective Effects on Reflux Esophagitis Model

Nam,

Song,

Lee

et al. 2023

Pharmacognosy Magazine

View full text Add to dashboard Cite

Objectives In the study, we investigated the effects of an Eriocheir sinensis water extract on a reflux esophagitis-induced rat model. Background The crab has been used as a traditional medicine and food source in many countries worldwide for a long time, and the crab contains various useful substances such as chitin, protein, calcium carbonate, etc. Materials and Methods All rats were fasted for 24 h and then orally pretreated with saline, Eriocheir sinensis (100 mg/kg), or ranitidine (40 mg/kg). Reflux esophagitis was induced by pylorus and forestomach ligation. Morphological changes in the mucosal damage in the esophagus were analyzed by the ImageJ program. Also, the expression of the inflammatory proteins and cytokine in the esophagus was measured by western blot assay to demonstrate the protective effects of Eriocheir sinensis in reflux esophagitis model. Histological analysis of esophagus was performed by hematoxylin & eosin (H&E) staining. Results In the reflux esophagitis induction group, the esophageal tissue damage caused by the induction of reflux was 60% of the whole. Eriocheir sinensis administration significantly ameliorated esophageal mucosal damage by 40%, also determined by histological evaluation of reflux esophagitis in rats. Eriocheir sinensis was also found to downregulate the expression levels of proteins such as cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), and tight junction protein (claudin-5) compared to the reflux esophagitis induction group. In addition, Eriocheir sinensis markedly attenuated the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and the phosphorylation of the inhibitor of NF-κB (IκBα). Conclusion These results indicated that Eriocheir sinensis suppressed the development of esophagitis and modulated inflammation by regulating NF-κB activation. Based on these findings, we concluded that Eriocheir sinensis can protect the esophageal mucosa from reflux esophagitis.

show abstract

Section: Discussionsupporting

confidence: 63%

Functional Properties of the Water Extract from Eriocheir sinensis (Chinese mitten crab) Via Evaluation of Protective Effects on Reflux Esophagitis Model

Nam,

Song,

Lee

et al. 2023

Pharmacognosy Magazine

View full text Add to dashboard Cite

show abstract

“…The protein levels of the groups are expressed relative to those of the normal rat (represented as 1). We followed the methods of Lee et al [26].…”

Section: Preparation Of Nuclear and Cytosolmentioning

confidence: 99%

Corni Fructus Alleviates UUO-Induced Renal Fibrosis via TGF-β/Smad Signaling

Lee

Shin

Roh

2022

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Renal fibrosis is a type of chronic kidney disease (CKD) induced by infiltration of inflammatory cells, myofibroblast accumulation, and ECM production in the kidney. From a long time ago, Corni Fructus (CF) is known to supplement the liver and kidney with its tepid properties. In this study, we investigated the renal protective mechanism of CF, which is known to supplement the kidney, in rat model of unilateral ureteral obstruction (UUO). After inducing UUO through surgery, the group was separated ( n = 8 ) and the drug was administered for 2 weeks; normal rats (normal), water-treated UUO rats (control), CF 100 mg/kg-treated UUO rats (CF100), and CF 200 mg/kg-treated UUO rats (CF200). As a result of histopathological examination of kidney tissue with H&E, MT, and PAS staining, it was confirmed that the infiltration of inflammatory cells and the erosion of collagen were relatively decreased in the kidneys treated with CF. Also, CF significantly reduced the levels of MDA and BUN in serum. As a result of confirming the expression of the factors through western blotting, CF treatment significantly reduced the expression of NADPH oxidase and significantly regulated the AMPK/LKB1/NF-κB pathway associated with inflammation. In addition, it downregulated the expression of major fibrotic signaling factors, such as α-SMA, collagen I, MMP-2, and TIMP-1, and significantly regulated the TGF-β1/Smad pathway, which is known as a major regulator of renal fibrosis. Taken together, these findings indicate that CF can alleviate renal fibrosis by regulating the TGF-β1/Smad pathway through inhibition of oxidative stress in UUO.

show abstract

“…Band densities were measured using ATTO Densitograph Software (ATTO Corporation, Tokyo, Japan) and quantified as the ratio to histone or β -actin. The protein levels of the groups are expressed relative to those of the normal rat (represented as 1) [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Scutellariae Radix and Citri Reticulatae Pericarpium Mixture Regulate PPARγ/RXR Signaling in Reflux Esophagitis

Lee

Shin

Park

et al. 2022

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Objective. Gastroesophageal reflux disease (GERD) is a gastrointestinal disorder in which stomach contents reflux into the esophagus, causing complications such as mucosal damage. GERD is a very common disease and is on the rise worldwide. The aim of this study was to assess the impact of a Scutellariae Radix and Citri Reticulatae Pericarpium mixture (SC) on esophageal mucosal injury in rats with chronic acid reflux esophagitis (CARE). Methods. After inducing reflux esophagitis through surgery, the group was separated and the drug was administered for 2 weeks: normal rats (Normal, n = 8), CARE-induced rats were treated with distilled water (Control, n = 8), CARE-induced rats were treated with vitamin E 30 mg/kg body weight (VitE, n = 8), CARE-induced rats were treated with SC 100 mg/kg body weight (SC100, n = 8), and CARE-induced rats were treated with SC 200 mg/kg body weight (SC200, n = 8). Results. SC treatment significantly reduced the degree of esophageal mucosal damage, significantly reduced levels of MDA and MPO, and inhibited the activation of the NF-κB inflammatory pathway by activating the PPARγ/RXR pathway. In addition, SC treatment significantly regulated the expression of arachidonic acid-related proteins (COX-1, COX-2, and PGE2) and modulated the MMP/TIMP proteins in reflux esophagitis. Conclusion. Consequently, SC improved the damage to the esophageal mucosa. Also, the anti-inflammatory effects of the SC suggested the inhibition of NF-κB pathway through the activation of the PPARγ/RXR pathway, thereby reducing the expression of inflammation-related cytokines.

show abstract

Protective Effects of Inflammation of Curcumae Longae Rhizoma 30% EtOH Extract on Acute Reflux Esophagitis Rats

Cited by 7 publications

References 47 publications

Functional Properties of the Water Extract from Eriocheir sinensis (Chinese mitten crab) Via Evaluation of Protective Effects on Reflux Esophagitis Model

Functional Properties of the Water Extract from Eriocheir sinensis (Chinese mitten crab) Via Evaluation of Protective Effects on Reflux Esophagitis Model

Corni Fructus Alleviates UUO-Induced Renal Fibrosis via TGF-β/Smad Signaling

Scutellariae Radix and Citri Reticulatae Pericarpium Mixture Regulate PPARγ/RXR Signaling in Reflux Esophagitis

Contact Info

Product

Resources

About