Protective Effects of Lactobacillus plantarum CCFM8610 against Acute Cadmium Toxicity in Mice

Abstract

bThis study evaluated the protective effects of Lactobacillus plantarum CCFM8610, a selected probiotic with good cadmium binding capacity, against acute cadmium toxicity in mice. Ninety mice were divided into prevention and therapy groups. In the prevention groups, CCFM8610 was administered at 10 9 CFU once daily for 7 days, followed by a single oral dose of cadmium chloride at 1.8 mg cadmium for each mouse. In the therapy groups, the same dose of CCFM8610 was administered for 2 days after an identical single dose of cadmium exposure. Mice that received neither cadmium nor culture or that received cadmium alone served as negative and positive controls, respectively. The effects of both living and dead CCFM8610 on cadmium ion concentrations in feces, liver, and kidney were determined. Moreover, the alterations in reduced glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and histopathology in the liver and kidney were investigated. The results showed that compared to the mice that received cadmium only, CCFM8610 treatment can effectively decrease intestinal cadmium absorption, reduce tissue cadmium accumulation, alleviate renal and hepatic oxidative stress, and ameliorate hepatic histopathological changes. Living CCFM8610 administered after cadmium exposure offered the most significant protection. Our results suggested that CCFM8610 is more effective against acute cadmium toxicity than a simple antioxidant treatment due to its special physiological functions and that it can be considered a new dietary therapeutic strategy against acute cadmium toxicity.

Protective Effects of Lactobacillus plantarum CCFM8610 against Chronic Cadmium Toxicity in Mice Indicate Routes of Protection besides Intestinal Sequestration

“…Our results show that when Cd is introduced orally, treatment with L. plantarum CCFM8610 decreases intestinal Cd absorption by increasing fecal Cd excretion (Fig. 2), an effect that can be attributed to its good Cd sequestration capability (17). In addition, CCFM8610 also decreases tissue Cd accumulation (Fig.…”

“…In our previous study, L. plantarum CCFM8610, a selected lactobacillus with good Cd-binding ability in vitro, was shown to have protective effects against acute Cd toxicity in mice (17). The intestinal Cd sequestration ability of CCFM8610 appeared to be important, but it was unclear whether all of its protective effects can be attributed to this single protection route.…”

“…Therefore, development of new strategies against Cd toxicity is still required. Recently, we have demonstrated that Lactobacillus plantarum CCFM8610, a selected probiotic with good Cd-binding capability, can significantly protect against acute Cd toxicity in both the livers and kidneys of mice (17). CCFM8610 can decrease intestinal Cd absorption, reduce Cd accumulation in tissues, and alleviate renal and hepatic oxidative stress.…”

Isolasi Bakteri Asam Laktat Berpotensi Probiotik Pada Dangke, Makanan Tradisional dari Susu Kerbau di Curio Kabupaten Enrekang

“…Penelitian invitro dan invivo, pada hewan percobaan maupun pada manusia telah banyak dilakukan untuk mengeksplorasi peranan probiotik dalam pencegahan maupun pengobatan penyakit, misalnya diare (Guandalini et al, 2011), Inflammatory Bowel Disease (Rauch et al, 2012), Irritable Bowel Syndrome (Ringel et al, 2011;Ki et al, 2012), asma (Jan et al, 2011;Jang et al, 2012), alergi (Drago et al, 2011;, obesitas (Luoto et al, 2010;Arora et al, 2012), diabetes tipe 2 (Ejtahed et al, 2012;Andersson et al, 2010), kanker kolorektal Chen et al, 2011;Appleyard et al, 2011), laktosa intoleran (Ojetti et al, 2010;He et al, 2008), depresi (Arseneault et al, 2011;Desbonnet et al, 2010;Bravo et al, 2011), osteoporosis (Yeo et al, 2010Rodrigues et al, 2012), reduksi kadar kolesterol , dan pengikatan ion logam berat (Buda et al, 2013;Zhai et al, 2013).…”

Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat

“…Probiotics have the ability to bind and detoxify some heavy metals [13]. This propriety could be a promising solution for heavy metal removal from water, liquid food, and from the body of humans and animals [14]. Besides heavy metal binding capacity, lactic acid bacteria also are known to have antioxidative properties, which may be another important characteristic for heavy metal toxicity protection [15].…”