Protective Effects of MiR-129-5p on Acute Spinal Cord Injury Rats

Abstract

Departmental sources Background: Spinal cord injury (SCI) is a severe devastating condition associated with serious disability and neurologic deficits. Aberrant micro RNA (miRNA) expression has been related to a variety of central nervous system diseases including SCI. In the present study, we aimed to discover the role of miR-129-5p on SCI. Material/Methods: An acute SCI rat model was induced, following the modified Allen method. A total of 36 rats were randomly assigned into 4 groups (n=9 in every group): Sham group; Model group (SCI+saline); SCI+NC group; and SCI+miR-129-5p group (100 nm solution, every 2 days). Basso-Beattie-Bresnahan (BBB) locomotor rating score was carried out to determine functional recovery. TUNEL (terminal dUTP nick-end labeling) staining was used to evaluate cell apoptosis. Hematoxylin and eosin staining was performed to assess the pathological state of spinal cord. Furthermore, western blot assay was conducted to measure the calpain1 and calpain2 expression. Results: Our data suggested that the expression level of miR-129-5p was markedly reduced in rats after SCI. Then miR-129-5p mimic was injected into the vertebral canal. We found that the SCI+miR-129-5p group had a high score in the BBB test compared with the SCI+NC group and the Model group. The overexpression of miR-129-5p obviously reduced tissue loss, damaged cells, and the number of TUNEL positive cells. Moreover, western blot assay exhibited that overexpression of miR-129-5p decreased calpain1, calpain2, and cleaved caspase-3 expression. Conclusions: Our findings suggested that overexpression of miR-129-5p improved neurological function by promoting functional recovery, reducing tissue loss and cell apoptosis in rats in an SCI model, possibly through downregulation of calpain1 and calpain2.

Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway

“…MiR-129-5p participates in regulating the proliferation, migration, and invasion of retinoblastoma cells and affects spinal cord injury [13,14]. In a study on the regulation of osteogenic differentiation, Valenti et al [15] found that physical exercise modulates miR-129-5p expression in progenitor cells and thus promotes osteogenesis.…”

Dihydrotanshinone I Alleviates Spinal Cord Injury via Suppressing Inflammatory Response, Oxidative Stress and Apoptosis in Rats

“…Spinal cord injury (SCI) is a serious complication of spine injury, putting patients in debilitating pathological conditions physically and psychologically, and may cause huge economic challenges to society [1][2][3]. SCI leads to physical damages, including infarction of the spinal cord, dyskinesia, and sensory deficits in the area below the injury, muscle wasting, chronic pain, and paralysis [4,5], resulting in acceleration of tissue edema, neural cell apoptosis, and the inflammatory cytokine release [6].…”

Optimization and characterization of miRNA-129-5p-encapsulated poly (lactic-co-glycolic acid) nanoparticles to reprogram activated microglia

“…21,24 Particularly, mimic miRNA-129-5p (miR-129) has received signicant attention as it can suppress cell apoptosis and microglia-induced inammatory response as well as polarize their phenotype through highmobility group protein B1 (HMGB1)/toll-like receptor4 (TLR4)/ NF-kB and leucine-rich alpha-2-glycoprotein (LRG1)/p38 mitogen-activated protein kinases (MAPK) pathways. [25][26][27][28] As a well-known maker of inammatory mediators and cancer therapy, HMGB1 is a direct target of miR-129. [29][30][31] Moreover, HMGB1 is passively released by necrotic and/or stressed cells as well as by immune cells including activated microglia.…”