Protective effects of papaverine salicylate in mouse ear dermatitis and PAF-induced rat paw oedema

Bernardis, E. De; Leonardi, Giuseppe; Caruso, A.; Cutuli, V. M. C.; Amico‐Roxas, M.

doi:10.1007/bf02014296

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…Zymosan has been found to be a reliable stimulus used in studies of inflammation in the ear, peritoneal cavity, joint and air-pouch models (for examples, see Dawson et al, 1991;Griffiths et al, 1991;Erdo et al, 1993;de Bernardis et al, 1994;Gegout et al, 1994Gegout et al, , 1995Rao et al, 1994). In addition to being a well-characterized inflammogen, there are a few reports that have used zymosan as a stimulus to evoke pain following intraperitoneal injection (Fujiyoshi et al, 1990;Heapy et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Intraplantar zymosan as a reliable, quantifiable model of thermal and mechanical hyperalgesia in the rat

Meller

Gebhart

1997

European Journal of Pain

104

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The study of the mechanisms of thermal and mechanical hyperalgesia produced in human inflammatory conditions is dependent on a reliable, consistent model. The present investigation shows that the intraplantar administration of zymosan in the rat hindpaw produces a reliable and quantifiable thermal and mechanical hyperalgesia accompanied by oedema that closely mimics the symptoms of inflammation in man. Prior to the intraplantar injection of zymosan, there was no significant difference in withdrawal latencies, mechanical withdrawal thresholds or paw thickness between the left and right hindpaws. The intraplantar injection of zymosan (0.313-6.25 mg), an extract from yeast, produced a dose- and time-dependent thermal and mechanical hyperalgesia, a robust oedema and, at the greatest doses, produced evidence of spontaneous pain. At the least dose of zymosan tested (0.313 mg), there was a slight oedema; oedema was greatest at dosages > or =2.5 mg and was always maximal by 30 min postinjection, irrespective of the dose. On the other hand, thermal and mechanical hyperalgesia showed a more complex dose- and time-dependence. Mechanical hyperalgesia did not appear until dosages > or =1.25 mg and was maximal by 5 mg. In addition, mechanical hyperalgesia showed a time-dependent progressive onset so that hyperalgesia was maximal by the 4-h testing time-point. In contrast, thermal hyperalgesia showed a biphasic nature with two apparent maximal time-points (30 min and 4 h). There was an early-phase thermal hyperalgesia (maximal by 30 min) that was dose-dependent at dosages > or =2.5 mg (not apparent at lower dosages) and a late-phase (maximal by 4 h) that was dose-dependent at dosages > or =0.0625 mg. At the greatest doses administered (5 and 6.25 mg), there was evidence of spontaneous pain from the time of injection for up to 4 h that was characterized by occasional spontaneous flicking of the hindpaw, but more usually by holding the paw in an elevated position for extended periods of time. In addition, at the greatest dose tested (6.25 mg), all rats showed evidence of licking, biting and shaking of the injected hindpaw for up to 30-45 min after injection. These data demonstrate that the intraplantar injection of zymosan is a reliable and quantifiable model of tonic pain characterized by a dose- and time-dependent thermal and mechanical hyperalgesia accompanied by a robust oedema. This model is likely to be a useful, reliable model in which to study further the central and peripheral mechanisms of hyperalgesia.

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Section: Discussionmentioning

confidence: 99%

Intraplantar zymosan as a reliable, quantifiable model of thermal and mechanical hyperalgesia in the rat

Meller

Gebhart

1997

European Journal of Pain

104

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“…Applied Engineering, Materials and Mechanics II polymorphonuclear infiltration, and plasma extravasation localized mainly to the dermis. [14,15] Two major components, terpinen-4-ol and sabinene, were demonstrated to have anti-inflammatory properties. They were shown to suppress the formation of nitric oxide and several inflammatory mediators such as TNF-α, IL-1, IL-8 and PGE 2 [16,17].…”

mentioning

confidence: 99%

Antifungal, Anti-Inflammatory and Cytotoxic Effects of <i>Zingiber cassumunar</i> Gel

Thaweboon

Kaypetch

2018

KEM

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This study aimed to investigate the antifungal, anti-inflammatory and cytotoxic effects of Zingiber cassumunar gel. The gel was prepared from essential oil of Zingiber cassumunar rhizome by the Thailand Institute of Scientific and Technological Research. Antifungal activity of the gel was firstly determined by the well diffusion method against Candida albicans ATCC 10238 and candida strain isolated from the patient’s lesion. Then, the Agar overlay technique was used to test the cytotoxicity of Z. cassumunar gel on mouse fibroblasts (ATCC clone 929) according to ISO 7405. For anti-inflammatory effect of the gel, TPA (carrageenan lambda type IV, 12-O-tetradecanoylphorbol-13- acetate)-induced mouse ear edema method was used. The results of well diffusion showed that Z. cassumunar gel was quite a potent antifungal agent against both strains of tested C. albicans with inhibition zones of 12-13 mm. In the cytotoxicity test, the gel exhibited no toxicity to cell culture. In addition, topical administration of Z. cassumunar gel could decrease mouse ear edema induced by TPA. At 30 and 60 min-time points, Z. cassumunar gel showed higher anti-inflammatory activity than triamcinolone which was used as reference anti-inflammatory drug. In conclusion, gel prepared from Z. cassumunar oil showed antifungal activity against both strains of C. albicans. In addition, its anti-inflammatory effect was demonstrated within 30 min by the TPA-induced mouse ear edema model. The gel was non-toxic to cell culture after 24-h incubation. Further studies are needed to clarify the safety and benefit of this gel for clinical use in the treatment of candidal infection and inflammation.

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Anti-proliferative and anti-inflammatory effects of 3β,6β,16β-Trihydroxylup-20(29)-ene on cutaneous inflammation

Horinouchi

Mendes

Nolte

et al. 2017

Journal of Ethnopharmacology

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Protective effects of papaverine salicylate in mouse ear dermatitis and PAF-induced rat paw oedema

Cited by 6 publications

References 21 publications

Intraplantar zymosan as a reliable, quantifiable model of thermal and mechanical hyperalgesia in the rat

Intraplantar zymosan as a reliable, quantifiable model of thermal and mechanical hyperalgesia in the rat

Antifungal, Anti-Inflammatory and Cytotoxic Effects of <i>Zingiber cassumunar</i> Gel

Anti-proliferative and anti-inflammatory effects of 3β,6β,16β-Trihydroxylup-20(29)-ene on cutaneous inflammation

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