Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms

Miller, Thomas A.

doi:10.1152/ajpgi.1983.245.5.g601

Cited by 374 publications

(255 citation statements)

References 124 publications

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“…These non-proteic compounds are important for maintaining the integrity of the gastric mucosa. Endogenous sulfhydryl compounds mediate the protective effects of prostaglandins against gastric mucosal injury (Miller 1983). Potentiation of the mucosal injury by NEM, an SH blocker, may be explained by the enhanced microvascular permeability in the gastric mucosa and inhibition of the gastric motility commonly associated with the prevention of mucosal injury (Takeuchi et al 1989).…”

Section: Resultsmentioning

confidence: 99%

Antiulcerogenic activity of Indigofera truxillensis Kunth

et al. 2006

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Cola-Miranda, M; Barbastefano, V.; Hiruma-Lima, C.A.; Calvo, T.R.; Vilegas, W. and Brito, A.R.M.S. Antiulcerogenic activity of Indigofera truxillensis Kunth. Biota Neotrop. Sep/Dec 2006 vol. 6, no. 3 http://www.biotaneotropica.org.br/v6n3/ pt/abstract?article+bn01006032006 ISSN 1676-0611The genus Indigofera (Fabaceae) is used in folk medicine to treat gastrointestinal pain. In this study, we investigated the antiulcerogenic properties of Indigofera truxillensis Kunth. Oral administration of MeOH extract did not produce any signals of acute toxicity. The antiulcerogenic activity was assessed in different models of acute gastric ulcers (100% ethanol, piroxicam 30 mg.kg -1 , hypothermic restraint stress and pylorus ligature) in mice and rats. The animals were treated with the drugs lanzoprazole (30 mg.kg -1 ) or cimetidine (100 mg.kg -1 ) as positive controls depending on the performed model. In another experiment with ethanol-induced ulcers in rats, N-ethylmaleimide (NEM), a sulfhydryl group blocker, was also used. The MeOH extract, at doses of 250, 500 and 1000 mg.kg -1 , inhibited the gastric lesions in all experiments: a) by 62%, 69% and 32%, respectively, in piroxicam-induced lesions, b) by 43%, 71% and 98%, in ethanol-induced lesions, c) by 69%, 64 and 89%, in hypothermic-restraint stress-induced lesions, d) by 73%, 82% and 84%, in pylorus ligature lesions. Significant changes in the total gastric acid levels were also found after intraduodenal administration of the MeOH extract in the ligated pylorus model. Pre treatment with NEM reduced partially the antiulcerogenic activity of the MeOH extract in ethanolinduced gastric lesions. This result indicates an increase in the levels of non-protein sulfhydryl groups by MeOH extract in the gastric mucosa. These results indicate that the MeOH extract has antisecretory and citoprotective effects that may be related to the presence of flavonoids detected by phytochemical analysis. O gênero Indigofera (Fabaceae) é utilizado na medicina tradicional para distúrbios gastrintestinais. Em nosso trabalho foi investigada a propriedade antiulcerogênica da Indigofera truxillensis Kunth. A administração oral do extrato metanólico (MeOH) não produziu efeitos tóxicos. A atividade antiulcerogênica foi avaliada em diferentes modelos agudos de úlcera gástrica (etanol 100%, piroxicam 30 mg.kg -1 , estresse por retenção e frio e ligadura do piloro) em camundongos e ratos. Os animais foram tratados com lansoprazol (30 mg.kg -1 ) ou cimetidina (100 mg.kg -1 ), que foram utilizados como controle positivo dependendo do modelo testado. Em outro experimento com úlcera induzida por etanol em ratos, N-etilmaleimida (NEM), um bloqueador dos compostos sulfidríla, também foi utilizado. O extrato metanólico, nas doses de 250, 500 e 1000 mg.kg -1 , inibiu significativamente as lesões gástricas em todos os experimentos: a) 62%, 69% e 32%, respectivamente, nas lesões gástricas induzidas por piroxicam, b) 43%, 71% e 98%, nas lesões gástricas induzidas por etanol, c) 69%, 64% e 89%, nas lesões ...

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Section: Resultsmentioning

confidence: 99%

Antiulcerogenic activity of Indigofera truxillensis Kunth

et al. 2006

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“…PGE2 exerts a number of biological actions on the stomach including inhibition of acid secretion, stimulation of HCO3-and mucus secretion and vasodilation of the gastric vasculature (Miller, 1983). Since specific PGE2 binding was detected in each fraction it is possible that these biological actions are initiated even by small degrees of PGE2 binding to the cells regulating these actions.…”

Section: Discussionmentioning

confidence: 99%

“…These studies have also been done using a stable analogue of prostacyclin or PGI2 (Iloprost). Both PGE2 and PGI2 have been detected in gastric mucosa of a number of animals (Robert, 1981;Miller, 1983) and administration of PGE2, PGI2 or Iloprost exert potent biological action on the stomachs of a number of animals and humans (Vischer & Casals-Stenzel, 1982;Miller, 1983). Furthermore this laboratory has characterized the binding of both prostanoids using gastric tissue from a number of species (Tepperman & Soper, 1981Tepperman, Soper & Emery, 1984).…”

Section: Introductionmentioning

confidence: 99%

Binding and biological actions of prostaglandin E2 and I2 in cells isolated from rabbit gastric mucosa.

Barr

Duncan

Kiernan

et al. 1988

The Journal of Physiology

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“…nism (20)(21)(22), but these effects are not without controversy (23,24). Mersereau and Hinchey (25) proposed that dissolution of the mucosal folds may be associated with the prevention of necrotic gastric lesions along the fold crests in the stomach after exposure to necrotizing agents.…”

Section: Discussionmentioning

confidence: 99%

Roles of gastric motility changes in cytoprotection induced by acetazolamide and cysteamine in rats.

et al. 1987

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Abstract-The present study was undertaken using acetazolamide (AZ) and cyste amine (Cys) to investigate the relationship between gastric motor activity and the phenomenon of "cytoprotection" in rats. Both AZ (10-100 mg/kg) and Cys (10-100 mg/kg), given either p.o. or s.c., significantly reduced the formation of gastric mucosal injury caused by HCI-ethanol (1 ml of 60% ethanol in 150 mM HCI, p.o.). The protective effect of Cys appeared within 10 min, reached the maximal levels 30 min later, while that of AZ appeared from 30 min after administration and became potent with a latency period after treatment. Neither indomethacin (IM: 5 mg/kg, s.c.) nor N-ethylmaleimide (NEM: 5 mg/kg, s.c.) significantly affected the protective effect of Cys, whereas that of AZ was almost totally antagonized by IM. Both AZ and Cys, given either intragastrically or s.c., significantly inhi bited gastric motor activity measured as intraluminal pressure recordings, but had minimal effect on acid and alkaline secretion. IM significantly attenuated the inhibitory effect of AZ on the motor activity, while NEM did not affect the inhibited motor responses caused by AZ and Cys. A significant relationship was found between the inhibitory effects of these two drugs on gastric motor activity and HCI-ethanol-induced mucosal injury, the correlation coefficient being 0.819 (P< 0.01). When the mucosal folds were visualized with Gentian Violet (1 ml of 0.5% v/v, p.o.), both AZ and Cys significantly prevented the localized staining along the mucosal folds, suggesting dissolution of the folds. These results suggest that both AZ and Cys protect the gastric mucosa against injury caused by HCI-ethanol, probably through a dissolution of mucosal folds due to inhibition of gastric motor activity.

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Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms

Cited by 374 publications

References 124 publications

Antiulcerogenic activity of Indigofera truxillensis Kunth

Antiulcerogenic activity of Indigofera truxillensis Kunth

Binding and biological actions of prostaglandin E2 and I2 in cells isolated from rabbit gastric mucosa.

Roles of gastric motility changes in cytoprotection induced by acetazolamide and cysteamine in rats.

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