Protective Effects of Pycnogenol against Ischemia Reperfusion-Induced Oxidative Renal Injury in Rats

Şehirli, Ahmet Özer; Şener, Göksel; Ercan, Feríha

doi:10.3109/08860220903085971

Cited by 28 publications

(25 citation statements)

References 57 publications

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“…PYC supplementation plays an active role in improving the control of the most common clinical aspects of psoriasis and in decreasing oxidative stress [25]. In a rat model of traumatic brain injury, PYC protects CA3-CA1 synaptic function [26] via its free radical scavenging and antioxidant activity [27]. Notably, our results showed that PYC pretreatment suppressed OGD/R-induced oxidative stress in primary rat astrocytes, along with a reduction in MDA content and ROS production, and an increase in SOD activity and MMP.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-κB and ERK1/2 MAPK Pathways

Xia¹,

Ji²,

Zhang³

2017

Cell Physiol Biochem

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Backgrounds/Aims: Pycnogenol (PYC) is a patented mix of bioflavonoids with potent anti-oxidant and anti-inflammatory properties. In this study, we investigated the effects of PYC on oxygen-glucose deprivation/reoxygenation (OGD/R)-induced injury in primary rat astrocytes. Methods: The primary rat astrocytes were randomly divided into 6 groups, blank control, OGD/R, OGD/R+PYC (10, 20, 40, and 60 µg/mL). The cell activity were detected by MTT and LDH assays, then the levels of oxidant products [malondialdehyde (MDA) and reactive oxygen species (ROS)] , antioxidants [superoxide dismutase (SOD)], mitochondrial membrane potential (MMP) and inflammatory cytokines were detected. In addition, the expression levels of apoptosis-related proteins (Bax, Bcl-2 and Cleaved caspase 3), proinflammatory factors (NF-κB p65), and p-ERK1/2 were measured by Western blot analysis. Results: The results showed that PYC incubation dose-dependently attenuated cell viability loss, LDH leakage, oxidative stress, inflammatory cytokines accumulation and cell apoptosis caused by OGD/R. Furthermore, PYC pretreatment dose-dependently suppressed OGD/R-induced NF-κB p65 nuclear translocation, NF-κB activity and ERK1/2 phosphorylation. Similarly to PYC, NF-κB inhibitor PDTC and ERK1/2 inhibitor PD098059 dramatically inhibited OGD/R-induced NF-κB activation, ERK1/2 phosphorylation, and ROS production, as well as TNF-α secretion. Conclusions: These findings revealed that PYC has neuroprotective effects against OGD/R-induced injury via NF-κB and ERK1/2 pathways in primary rat astrocytes.

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Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-κB and ERK1/2 MAPK Pathways

Xia¹,

Ji²,

Zhang³

2017

Cell Physiol Biochem

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“…PYC ® was reported to reduce IR-induced renal injury and preserve renal function. 42 During the reperfusion phase of the liver, emerging reactive oxygen radicals activate some mediators and can cause an inflammatory response and tissue damage. For this reason, AST, ALT, and LDH levels may increase.…”

Section: Discussionmentioning

confidence: 99%

The Ameliorative Effects of Pycnogenol<sup>®</sup> on Liver Ischemia-Reperfusion Injury in Rats

Tokaç¹,

Bacanlı²,

Dumlu³

et al. 2017

tjps

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“…MPO is a hemecontaining peroxidase abundantly expressed in PMN. 6 Excessive generation of MPO-derived oxidants has been linked to tissue damage in many diseases, especially those characterized by acute or chronic inflammation. 15 Because MPO activities are a reliable indicator for PMN sequestration, MPO activities in kidney, pancreas, and myocardium are used to assess the degree of these tissues PMN infiltration.…”

Section: Discussionmentioning

confidence: 99%

“…These findings suggest that ARF can induce the heart and pancreas injury. [3][4][5] Myeloperoxidase (MPO) levels are a reliable and quantitative marker for polymorphonuclear neutrophil (PMN) accumulation in tissues, 6 and membrane pump activity is an indicator for energy metabolism and ion transport of cells. 7 To test this hypothesis and to improve our understanding of the cellular mechanisms underlying the heart and pancreas injury caused by ARF and determined by the MPO and membrane pump activities in heart, pancreas, and kidney, we used our established model of tissue from rabbits subjected to ARF.…”

Section: Introductionmentioning

confidence: 99%

The mechanism of myocardium and pancreas injury in rabbits with acute renal failure might be related to myeloperoxidase and membrane pump activities

et al. 2010

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LABORATORY STUDYThe mechanism of myocardium and pancreas injury in rabbits with acute renal failure might be related to myeloperoxidase and membrane pump activitiesThe mechanism of myocardium and pancreas injury in acute renal failure Zi-Gang Zhao, Chun-Yu Niu, Yu-Ping Zhang, Rui Han, Ya-Li Hou, Bao-liang Li, and Shu-ting Du Institute of Microcirculation, Hebei North University, Zhangjiakou 075000, Hebei, PR China AbstractThere is increasing evidence indicating that the distant organ injury is a major contributor of high mortality in patients subjected to acute renal failure (ARF). However, sources and mechanisms that ARF causes distant organ injury remain to be determined. The aim of this study is to explore the mechanism from polymorphonuclear neutrophil (PMN) sequestration and membrane pump suppression. To achieve this, we examined myeloperoxidase (MPO), a marker of PMN accumulation in tissues, and membrane pump activities of heart, pancreas, and kidney in two ARF rabbit models. Rabbits are randomly assigned to control, HgCl 2 -treated, and glycerin-treated groups. ARF animal models are established by hypodermic injection of 1% HgCl 2 with 1.3 mL/kg bodyweight (bw) in HgCl 2 -treated group or intramuscular injection of 50% glycerin with 10 mL/kg bw in glycerin-treated group, respectively, and all animals in each group are further divided into 12 h, 24 h, and 48 h subgroups with each consisting of six rabbits. Six healthy rabbits serve as control group. Results have shown that MPO activities of kidney, myocardium, and pancreas in two model groups were significantly increased than control group at diverse time points. Membrane pump activities of kidney in two model groups are significantly lower than the control group at multiple time points. Moreover, Na -ATPase activities of myocardium and pancreas in two model groups are gradually declined with the development of ARF. These findings suggest that PMN sequestration and membrane pump suppression plays an important role in the pathogenesis of ARF and also a major mechanism of myocardium and pancreas injury during the process of ARF.

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Protective Effects of Pycnogenol against Ischemia Reperfusion-Induced Oxidative Renal Injury in Rats

Cited by 28 publications

References 57 publications

Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-κB and ERK1/2 MAPK Pathways

Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-κB and ERK1/2 MAPK Pathways

The Ameliorative Effects of Pycnogenol<sup>®</sup> on Liver Ischemia-Reperfusion Injury in Rats

The mechanism of myocardium and pancreas injury in rabbits with acute renal failure might be related to myeloperoxidase and membrane pump activities

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