Protective Effects of Pyruvic Acid Salt Against Lithium Toxicity and Oxidative Damage in Human Blood Mononuclear Cells

Plotnikov, Evgenii; Losenkov, I.; Epimakhova, Elena V.; Bohan, Nikolay

doi:10.15171/apb.2019.035

Cited by 10 publications

(8 citation statements)

References 22 publications

(22 reference statements)

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“…In the biochemical industry, it can be used as a synthetic precursor for n -butanol, l -alanine, l -tyrosine, isobutanol, and so on. In the pharmaceutical industry, it can be used to synthesize isoniazid pyruvate, vitamin B, and 4-phenyl-2-oxobutyric acid ethyl ester and has antioxidant effects . In the agricultural field, it can be used to synthesize pesticides such as cycloprothrin .…”

Section: Synthesis and Application Of Pyruvatementioning

confidence: 99%

“…In the pharmaceutical industry, it can be used to synthesize isoniazid pyruvate, 33 vitamin B, 34 and 4-phenyl-2oxobutyric acid ethyl ester 35 and has antioxidant effects. 36 In the agricultural field, it can be used to synthesize pesticides such as cycloprothrin. 37 In the food field, it is an important food additive and dietary supplement.…”

Section: ■ Synthesis and Application Of Pyruvatementioning

confidence: 99%

See 1 more Smart Citation

Cell-Free Biosynthesis System: Methodology and Perspective of in Vitro Efficient Platform for Pyruvate Biosynthesis and Transformation

Tang

Liao

et al. 2021

ACS Synth. Biol.

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The modification of intracellular metabolic pathways by metabolic engineering has generated many engineered strains with relatively high yields of various target products in the past few decades. However, the unpredictable accumulation of toxic products, the cell membrane barrier, and competition between the carbon flux of cell growth and product synthesis have severely retarded progress toward the industrial-scale production of many essential chemicals. On the basis of an in-depth understanding of intracellular metabolic pathways, scientists intend to explore more sustainable methods and construct a cell-free biosynthesis system in vitro. In this review, the synthesis and application of pyruvate as a platform compound is used as an example to introduce cell-free biosynthesis systems. We systematically summarize a proposed methodology workflow of cell-free biosynthesis systems, including pathway design, enzyme mining, enzyme modification, multienzyme assembly, and pathway optimization. Some new methods, such as machine learning, are also mentioned in this review.

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Section: Synthesis and Application Of Pyruvatementioning

confidence: 99%

Section: ■ Synthesis and Application Of Pyruvatementioning

confidence: 99%

Cell-Free Biosynthesis System: Methodology and Perspective of in Vitro Efficient Platform for Pyruvate Biosynthesis and Transformation

Tang

Liao

et al. 2021

ACS Synth. Biol.

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“…Numerous studies have reported that oxidative stress is the most important mechanism for lithium-induced damage [10][11][12]. In this context, several studies demonstrated the role of lithium-induced oxidative stress on testicular tissue and the semen of animals and humans [10,13].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Coexposure of Silymarin with Lithium Chloride Preserves Human Sperm Quality: A Useful Approach to Reduce Oxidative Damages

Nasimi,

Momeni,

Etemadi

et al. 2023

Andrologia

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Lithium, a common component in various mood-stabilizing drugs, induces adverse effects on spermatozoa and human fertility. Oxidative stress, as a possible mechanism involved in lithium toxicity, can be reversed using antioxidants. Therefore, in this study, the effects of silymarin as a potent antioxidant were evaluated on human spermatozoa treated with lithium. Human spermatozoa were divided into five groups: (1) spermatozoa at 0 min, (2) spermatozoa at 180 min (control group), (3) spermatozoa treated with lithium chloride (500 μM) for 180 min, (4) spermatozoa were treated with silymarin (100 μM) + lithium chloride (500 μM) for 180 min, and (5) spermatozoa treated with silymarin (100 μM) for 180 min. Sperm motility and viability, the levels of malondialdehyde (MDA) and ferric reducing antioxidant power (FRAP), and the activity of antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase) were evaluated in these groups. The results showed that sperm motility and viability significantly ( p < 0.001 ) decreased in the group treated with lithium. In addition, a significant increase ( p < 0.001 ) was observed in MDA levels, while FRAP amount and the activity of antioxidant enzymes decreased significantly ( p < 0.001 ) in this group. Interestingly, these results were significantly ( p < 0.001 ) reversed in the group treated with silymarin + lithium. The finding of the present study showed that oxidative stress plays a crucial role in lithium toxicity in human spermatozoa, and the coapplication of silymarin as a potent antioxidant with lithium can protect human sperm against oxidative stress-induced damage.

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“…PA calcium salts were demonstrated to speed up fatty acid metabolism, while also helping to reduce serum cholesterol levels [6]. PA also possesses antioxidant effects and could be used as a nutraceutical to treat diabetes II [7,8].…”

Section: Introductionmentioning

confidence: 99%

The Production of Pyruvate in Biological Technology: A Critical Review

Wei

et al. 2022

Microorganisms

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Pyruvic acid has numerous applications in the food, chemical, and pharmaceutical industries. The high costs of chemical synthesis have prevented the extensive use of pyruvate for many applications. Metabolic engineering and traditional strategies for mutation and selection have been applied to microorganisms to enhance their ability to produce pyruvate. In the past decades, different microbial strains were generated to enhance their pyruvate production capability. In addition to the development of genetic engineering and metabolic engineering in recent years, the metabolic transformation of wild-type yeast, E. coli, and so on to produce high-yielding pyruvate strains has become a hot spot. The strategy and the understanding of the central metabolism directly related to pyruvate production could provide valuable information for improvements in fermentation products. One of the goals of this review was to collect information regarding metabolically engineered strains and the microbial fermentation processes used to produce pyruvate in high yield and productivity.

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Protective Effects of Pyruvic Acid Salt Against Lithium Toxicity and Oxidative Damage in Human Blood Mononuclear Cells

Cited by 10 publications

References 22 publications

Cell-Free Biosynthesis System: Methodology and Perspective of in Vitro Efficient Platform for Pyruvate Biosynthesis and Transformation

Cell-Free Biosynthesis System: Methodology and Perspective of in Vitro Efficient Platform for Pyruvate Biosynthesis and Transformation

In Vitro Coexposure of Silymarin with Lithium Chloride Preserves Human Sperm Quality: A Useful Approach to Reduce Oxidative Damages

The Production of Pyruvate in Biological Technology: A Critical Review

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