Protective effects of resveratrol against mancozeb induced apoptosis damage in mouse oocytes

Abstract

Mancozeb, a mixture of ethylene-bis-dithiocarbamate manganese and zinc salts, is one of the most widely used fungicides in agriculture. Mancozeb could lead to mitochondria dysfunction, cellular anti-oxidation enzymes depletion and apoptotic pathways activation. Previous studies indicated the exposure of mancozeb through mother would lead to irregular estrous cycles, decreased progesterone levels, reduced litter sizes, and more frequent delivery of dead fetuses. In this study, we investigated mancozeb inducing reproductive toxicity, especially focusing on its apoptotic effect and epigenetic modifications. We also showed that resveratrol, a kind of phytoalexin found in peanuts and grapes, can alleviate mancozeb's adverse effects, such as declined fertility, decreased ovary weight and primary follicles. Besides, mancozeb treated oocytes displayed suboptimal developmental competence and this can also be improved by treatment of resveratrol. More detailed investigation of these processes revealed that mancozeb increased reactive oxygen species, causing cell apoptosis and abnormal epigenetic modifications, and resveratrol can block these cytotoxic changes. Collectively, our results showed that resveratrol can alleviate mancozeb induced infertility and this was mainly through the correction of apoptotic tendency and the abnormity of cellular epigenetic modification.

Association between Female Reproductive Health and Mancozeb: Systematic Review of Experimental Models

“…Oral mancozeb exposure (800 mg/kg/day) also reduces litter size and weight with a relevant decrease in ovary weight [45]. In this context, there was also an increase in atretic follicles and a reduction in healthy oocytes.…”

“…In this context, there was also an increase in atretic follicles and a reduction in healthy oocytes. Liu et al also reported ultrastructural changes in Germinal Vesicle (GV) oocytes due to mancozeb administration [45]. Noteworthy, is the decrease in the number of pronuclei and two-cells of parthenogenetic activated oocytes and the alteration in actin expression levels [45].…”

“…Liu et al also reported ultrastructural changes in Germinal Vesicle (GV) oocytes due to mancozeb administration [45]. Noteworthy, is the decrease in the number of pronuclei and two-cells of parthenogenetic activated oocytes and the alteration in actin expression levels [45]. There is an increase in apoptotic pathways and ROS production, with an abnormal mitochondrial distribution.…”

“…There is an increase in apoptotic pathways and ROS production, with an abnormal mitochondrial distribution. Finally, mancozeb exposure is strongly linked to epigenetic modifications, which can compromise female fecundability [45]. Other studies suggest the potential of ethylene thiourea to influence important biomarkers of ovarian aging, in CD-1 mice exposed to 0.1, 1, and 10 mg/kg/day, from conception to postnatal day 21 [46].…”

Effect of Mancozeb on Mustard (Brassica juncea L.): An In-vitro study

“…The resulting symptoms are convulsions, slurred speech, confusion, slowed heartbeat etc. Mancozeb is reported to be an established carcinogen (FAO/WHO 1993, Vettorazzi et al 1995, Liu et al 2016. Mancozeb is reported to cause teratogenic, carcinogenic and goitrogenic effects on mammals.…”