Protective effects of rosmarinic acid against azoxymethane‐induced colorectal cancer in rats

Abstract

Colorectal cancer (CRC) incidence is increasing gradually and has been become one of the most common cancers worldwide. Hence, it is important to discover cheap, naturally occurring compounds to be effective in suppressing the devastating effect of colonrelated tumors. Rosmarinic acid (RA), one of the compounds of plant origin, possesses attractive features for use as an agent for cancer prevention and treatment. This study investigated the ability of RA to prevent azoxymethane (AOM)-induced rat colon carcinogenesis by evaluating the effect of RA on tumor formation and circulatory oxidantantioxidant status. Moreover, plasma levels of adiponectin (APN) monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) were detected by enzyme linked immunosorbent assay. The animals were divided into three groups: Control, AOM, and AOM + RA. Rats were fed a modified pellet diet (15.8% peanut oil was added to the standard diet) during the experimental period. Colon cancer was formed by applying 15 mg/kg AOM intraperitoneal once a week for 4 weeks in both the CRC group and AOM + RA group. Besides AOM, AOM + RA group received 5 mg/kg body weight RA orally every day during the study. The results showed that adenocarcinoma rates formed 87.5% of the AOM group. With treatment of RA, a reduction in the incidence of adenocarcinoma was observed in the AOM + RA group. The plasma MCP-1, IL-6, and TO levels were significantly higher, APN and TAS levels were significantly lower in the AOM group with respect to controls. In addition, there was a significant increase in TAS levels in the RA treatment group compared to the AOM group. These findings suggested that RA may be beneficial in preventing AOM-induced colon carcinogenesis formation.

Ratlarda Rosmarinik Asit’in Akut Pankreatit Üzerindeki Koruyucu Etkinliğinin İncelenmesi

“…Ilhan et al showed in their study that there was a significant increase in TAS levels of the group receiving rosmarinic acid [26][27] . However, in this study, we found that there was no statistically significant difference as a result of the evaluation of TAS levels in both tissue and serum.…”

Biomedical features and therapeutic potential of rosmarinic acid

“…RA/ZnO/chitosan nanoparticles exhibited high efficiency in combating methamphetamine elevation in casp3a mRNA levels and oxidative changes. Ilhan et al ( 2022 ) reported protective effects of azoxymethane-induced rat colon carcinogenesis and anti-oxidant/oxidant balance. Fuster et al ( 2021 ) formulated RA-loaded silk-fibroin nanoparticles to investigate the anti-tumor potential in human cervical and breast cancer cells.…”

Rosmarinic Acid and Related Dietary Supplements: Potential Applications in the Prevention and Treatment of Cancer

“…In AOM and dextran sulfate sodium-induced colorectal carcinogenesis animal models, interleukin-6 (IL-6) levels and progression of colitis-associated colon cancer were decreased by RA. The mechanism involved the downregulation of Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) and signal transducer and activator of transcription 3 (STAT3) [ 9 , 79 ]. RA prevents colorectal carcinogenesis due to antioxidation and anti-inflammatory effects.…”

“…In addition, RA attenuated DMH-induced upregulation of cytochrome P450 (CYP450) [ 74 , 77 ]. Second, RA inhibited the release of TNF-α, IL-6, and COX-2 pro-inflammatory factors [ 76 , 79 ]. The anti-inflammatory effect of RA has been related to the inhibition of TLR4/NF-κB and STAT3 [ 9 ].…”