Protective effects of Salvia plebeia compound homoplantaginin on hepatocyte injury

Qu, Xian‐Jun; Xue, Xindong; Wang, Yuanshu; Song, Meijuan; Liu, Lili; Xie, Ying; Cheng, Ye; Liu, Xiangjuan; Qiu, Lulu; Xiang, Lan; Gao, Jianjun; Zhang, Xiaofan; Cui, Shu‐Xiang

doi:10.1016/j.fct.2009.04.032

Cited by 56 publications

(33 citation statements)

References 28 publications

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“…Second, the extract of S. plebeia possesses antiangiogenic, antinociceptive, antiinflammatory, antifungal and antioxidant activities 33,45,46 . Third, the homoplantaginin isolated from S. plebeia was reported to be protective against hepatocyte injury 47 . Finally, in combination with other herbal ingredients [the seeds of Plantago asiatica L., the roots of Serissa serissoides (DC.)…”

Section: S Plebeiamentioning

confidence: 99%

An ethnopharmacological investigation of medicinal Salvia plants (Lamiaceae) in China

Zhang

et al. 2013

Acta Pharmaceutica Sinica B

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In China, over 40 species of the genus Salvia have been used as medicinal plants for various diseases, some for thousands of years. Recently, research has focused on the biological activities of Salvia medicinal plants used in traditional chinese medicine (TCM). However, to date a scientific survey of the genus Salvia in China has not been carried out. In this paper, we report the results of 10 field surveys of Salvia medicinal plants collected in 17 provinces including detailed information on their local names, growing environment, distribution and therapeutic effects. We also summarize the results of research on the materia medica, phytochemistry and pharmacology of some of the important Salvia medicinal plants. Our study reveals that 35 Salvia plants have been used in TCM in different regions of China, including 20 species used as Danshen to treat heart diseases, and 15 species used to treat a range of other conditions including gynecological diseases, muscular or skeletal problems, hepatitis, urological diseases, and mouth and eye conditions. It is clear that some species of Salvia L. possess significant pharmacological activity in the context of ethnopharmacological knowledge, especially in the treatment of heart disease. Further studies of the phytochemistry and pharmacology of Salvia species will no doubt improve their medical utilization and contribute to the development of new natural drugs.

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Section: S Plebeiamentioning

confidence: 99%

An ethnopharmacological investigation of medicinal Salvia plants (Lamiaceae) in China

Zhang

et al. 2013

Acta Pharmaceutica Sinica B

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“…CCC has the following distinct advantages over other column chromatography: rapid separation, high recovery, low solvent consumption, and low risk of sample denaturation [17]. In addition, due to the liquid nature of the stationary phase, several operation modes, such as Caffeic acid (1), 6-hydroxyluteuolin-7-glucoside (2), 5,7,3 ,4 -tetrahydroxy-6-methoxyflavanone-7-glucoside (3), nepitrin (4), rosmarinic acid (5), homoplantaginin (6), nepetin (7), hispidulin (8), and 5,6,7,4 -tertrahydroxyflavone (9).…”

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confidence: 99%

Separation of nine compounds fromSalvia plebeiaR.Br. using two-step high-speed counter-current chromatography with different elution modes

et al. 2014

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Nine compounds were successfully separated from Salvia plebeia R.Br. using two-step high-speed counter-current chromatography with three elution modes. Elution-extrusion counter-current chromatography was applied in the first step, while classical counter-current chromatography and recycling counter-current chromatography were used in the second step. Three solvent systems, n-hexane/ethyl acetate/ethanol/water (4:6.5:3:7, v/v), methyl tert-butyl ether/ethyl acetate/n-butanol/methanol/water (6:4:1:2:8, v/v) and n-hexane/ethyl acetate/methanol/water (5:5.5:5:5, v/v) were screened and optimized for the two-step separation. The separation yielded nine compounds, including caffeic acid (1), 6-hydroxyluteuolin-7-glucoside (2), 5,7,3',4'-tetrahydroxy-6-methoxyflavanone-7-glucoside (3), nepitrin (4), rosmarinic acid (5), homoplantaginin (6), nepetin (7), hispidulin (8), and 5,6,7,4'-tertrahydroxyflavone (9). To the best of our knowledge, 5,7,3',4'-tetrahydroxy-6-methoxyflavanone-7-glucoside and 5,6,7,4'-tertrahydroxyflavone have been separated from Salvia plebeia R.Br. for the first time. The purities and structures of these compounds were identified by high-performance liquid chromatography, electrospray ionization mass spectrometry, (1)H and (13)C NMR spectroscopy. This study demonstrates that high-speed counter-current chromatography is a useful and flexible tool for the separation of components from a complex sample.

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“…Chemical fingerprint and quantitative analysis showed that seven bioactive compounds of homoplantaginin, hispidulin, luteolin, nepetin, caffeic acid, luteolin-7-glucoside, and nepetin-7-glucoside contained as constituents in Salvia plebeia (21). The major compound of Salvia plebeia, homoplantaginin, has a protective and therapeutic effect on hepatocyte injury, which might be associated with its antioxidant activity (21,22). Unfortunately, the present study did not identify the constituents of SWE.…”

Section: Discussionmentioning

confidence: 44%

Sage weed (Salvia plebeia) extract antagonizes foam cell formation and promotes cholesterol efflux in murine macrophages

Park

Kim

Kang

et al. 2012

International Journal of Molecular Medicine

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Abstract. Lipid-laden peripheral tissue cells release cholesterol to an extracellular acceptor such as high-density lipoprotein (HDL). Foam cells are formed at the first stage of atherosclerosis development. This study investigated whether sage weed (Salvia plebeia) extract (SWE) influences cholesterol handling of J774A1 murine macrophages. A murine macrophage cell line, J774A1, was used in this study. Oxidized low-density lipoproteins (LDL) treatment was used for foam cell formation, which was confirmed using Oil red O staining. The oxidized LDL uptake and cholesterol efflux from lipid-laden foam cellassociated proteins were detected by western blot analysis. Also, transcriptional levels of these associated genes were examined using reverse transcription-PCR. Also, cholesterol efflux was measured using NBD-cholesterol efflux assay. Non-toxic SWE at ≥10 µg/ml attenuated scavenger receptor (SR)-B1 expression of macrophages induced by oxidized LDL for 6 h, which was achieved at its transcriptional levels. Consistently, SWE suppressed oxidized LDL-stimulated cellular lipid accumulation and foam cell formation due to downregulated SR-B1. SWE upregulated the protein expression and mRNA levels of ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) in lipid-laden foam cells, both responsible for cholesterol efflux. In addition, SWE promoted apolipoprotein E (apoE) secretion from oxidized LDL-induced foam cells. Cholesterol efflux was enhanced by ≥10 µg/ml SWE most likely through the induction of ABCA1 and ABCG1 and the secretion of apoE. Although 10 µM homoplantaginin, a compound mainly present in sage weeds, did not influence cellular expression of ABCA1 and ABCG1, it suppressed oxidized LDL-enhanced SR-B1 induction and foam cell formation. These results demonstrate that SWE antagonized oxidized LDL uptake and promoted cholesterol efflux in lipidladen macrophages. Therefore, SWE may serve as a protective therapeutic agent against the development of atherosclerosis.

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Protective effects of Salvia plebeia compound homoplantaginin on hepatocyte injury

Cited by 56 publications

References 28 publications

An ethnopharmacological investigation of medicinal Salvia plants (Lamiaceae) in China

An ethnopharmacological investigation of medicinal Salvia plants (Lamiaceae) in China

Separation of nine compounds fromSalvia plebeiaR.Br. using two-step high-speed counter-current chromatography with different elution modes

Sage weed (Salvia plebeia) extract antagonizes foam cell formation and promotes cholesterol efflux in murine macrophages

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