Emerging studies demonstrate that long noncoding RNAs (lncRNA) participate in the regulation of various cancers. In the current study, a novel has been identified and explored in esophageal squamous cell carcinoma (ESCC). To discover a new regulatory circuitry in which RNAs crosstalk with each other, the transcriptome of lncRNA-miRNA-mRNA from ESCC and adjacent nonmalignant specimens were analyzed using multiple microarrays and diverse bioinformatics platforms. The functional role and mechanism of a novel were further investigated by gain-of-function and loss-of-function assays and An ESCC biomarker panel, consisting of, , and, was validated by qRT-PCR and hybridization using samples from 148 patients. as an oncogene is highly expressed in ESCC tissues and cell lines, and promotes ESCC cell proliferation and metastasis. Mechanistically, promotes expression of transcription factor Snail1 by competitively binding, resulting in the epithelial-mesenchymal transition (EMT) cascade. Moreover, also induces FSCN1 expression by sponging and upregulation of mRNA-stabilizing protein HuR, which further promotes ESCC invasion cascades. We also discovered and validated a clinically applicable ESCC biomarker panel, consisting of ,, and , that is significantly associated with overall survival and provides additional prognostic evidence for ESCC patients. As a novel regulator, plays an important role in ESCC cell proliferation and metastasis. The regulatory axis provides bona fide targets for anti-ESCC therapies. .

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Aberrant sialylation and altered sialyltransferase (ST) expressions are frequently found in many types of cancer. In this study, we examined the expressions of α 2,3-linked sialic acid residues and STs mRNA in human gastric cancer tissues. The relationship between the levels of α 2,3-linked sialic acid residues and the expression of STs mRNA was also analyzed. Maackia amurensis leukoagglutinin (MAL) specific for α 2,3-linked sialic acid was used for immunohistochemical staining. All gastric cancer tissues were positive for MAL staining, whereas adjacent normal tissues were negative. The extent of MAL staining was significantly correlated with the behavior of gastric cancer. The expression of STs mRNA was examined by real-time quantitative reverse transcription-polymerase chain reaction assay. The levels of ST3Gal IV and ST6GalI in gastric cancer tissues were significantly increased compared with those in adjacent normal tissues (P < 0.05), whereas the levels of ST3Gal I, ST3Gal III, and ST3Gal VI were not increased. Furthermore, the high expression of ST3Gal IV was closely related to the extent of MAL staining (P < 0.05) unlike that of ST6Gal I. These results indicated that gastric cancer tissues expressed high levels of α 2,3-linked sialic acid residues, ST3Gal IV, and ST6Gal I. The high expression of ST3Gal IV may contribute to the expression of α 2,3-linked sialic acid residues, which is associated with the malignant behavior of gastric cancer cells.

A new homoisoflavan, 7,3',4'-trihydroxy-3-benzyl-2H-chromene (1), was isolated from the dried heartwood of Caesalpinia sappan L., together with seven known phenolic compounds. The structure of the new compound (1) was determined on the basis of spectroscopic analysis.