Protective effects of silybin on human umbilical vein endothelial cell injury induced by H2O2 in vitro

Wang, Yunkai; Hong, Ya-jun; Huang, Zhichuan

doi:10.1016/j.vph.2005.06.002

Cited by 33 publications

(20 citation statements)

References 41 publications

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“…Because of the very unstable chemical nature of NO, endothelium-derived NO can be rapidly inactivated by oxygen-derived free radicals due to the formation of the complex between NO and O 2 ·-, and thus, an enhanced production of ROS appears to be involved in the accelerated breakdown of NO. Excess vascular oxidative stress leads to the impairment of NO production, resulting in reduced NO in endothelial cells [46]. Therefore, we detected the concentrations of ET-1 and NO to assess HUVECs' function in regulating vasoconstriction and vasodilation and injury of HUVECs.…”

Section: Discussionmentioning

confidence: 99%

Antisense Oligodeoxynucleotide Against Tissue Factor Inhibits Human Umbilical Vein Endothelial Cells Injury Induced by Anoxia-Reoxygenation

Yin

Luo²,

Yao³

et al. 2010

Cell Physiol Biochem

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Aims: The role of antisense oligodeoxynucleotide against tissue factor (aODN/TF) in cultured human umbilical vein endothelial cells (HUVECs) subjected to anoxia-reoxygenation (A/R) was investigated. Methods: HUVECs were divided randomly into control group, A/R group, aODN/TF+A/R group, sense oligodeoxynucleotide (sODN/TF) + A/R group and mismatched oligodeoxynucleotide (mODN/TF) + A/R group, in the latter 3 groups, HUVECs were transfected with aODN/TF, sODN/TF and mODN/TF respectively. HUVECs in all A/R groups underwent 3 hrs of anoxia and followed by 2 hrs of reoxygenation. In order to investigate the potential mechanisms of how increased TF may contribute to A/R injury in HUVECs, another set of HUVECs were incubated with human recombinant active site blocked factor VII (FVIIai) during A/R. Results: After A/R, TF expression at both mRNA and protein level was increased, furthermore, cell viability and the concentrations of SOD, GSH-PX and NO were declined, while LDH, MDA and ET-1 were overproduced (P<0.05 to 0.001 versus control group). In HUVECs of aODN/TF+A/R group, however, TF expression was inhibited, while the declined cell viability and the concentrations of SOD, GSH-PX, NO as well as the enhanced LDH, MDA and ET-1 levels occurred during A/R were ameliorated and reversed effectively (P<0.05 to 0.01 versus those in other A/R groups). The results also showed that ROS was increased and PAR-1, PAR-2, p38 MAP kinase and p42/44 MAP kinase were all activated after A/R (P<0.001 versus HUVECs under normoxia), while FVIIai inhibited the increment of ROS, PAR-1, PAR-2, p38 MAP kinase and p42/44 MAP kinase, and improved the changes of TF:C, MDA, SOD, GSH-PX, cell viability and LDH occurred during A/R (P<0.05 to 0.001 versus HUVECs without FVIIai treatment). Conclusion: Tissue factor plays an important role in the development of HUVECs injury induced by anoxia-reoxygenation, inhibition of TF with antisense oligodeoxynucleotide is an effective approach to ameliorate the damage.

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Section: Discussionmentioning

confidence: 99%

Antisense Oligodeoxynucleotide Against Tissue Factor Inhibits Human Umbilical Vein Endothelial Cells Injury Induced by Anoxia-Reoxygenation

Yin

Luo²,

Yao³

et al. 2010

Cell Physiol Biochem

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“…Using water channels, H 2 O 2 cross cell membrane, reach nucleus, attack DNA, and single strand breaks occur. [11] In the present study, we treated 3T3-L1 cells with 100 µM of H 2 O 2 for 30 min showed more DNA damage. Subsequent treatment with different concentration of oil showed a decrease in tail length.…”

Section: Discussionmentioning

confidence: 65%

Antigenotoxic and apoptotic activities of essential oil of Atalantia monophylla Correa

Thirugnanasampandan¹,

Ramya²,

Gogulramnath³

2016

Indian J Pharmacol

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Objective:To study antigenotoxic and apoptotic activities of hydrodistilled essential oil from the leaves of Atalantia monophylla Correa.Materials and Methods:Antigenotoxic activity of essential oil was tested against hydrogen peroxide (100 μM)-induced deoxyribonucleic acid (DNA) damage in 3T3-L1 cells. Cervical cancer cell (HeLa) growth inhibitory effect of essential oil was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay. Annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI), Hoechst 33258, and acridine orange/ethidium bromide (AO/EtBr) staining techniques were used to identify apoptosis.Results:DNA protecting the activity of A. monophylla essential oil was high at 125 μg/mL. HeLa cell growth was inhibited dose-dependently and inhibitory concentration 50% was calculated as 43.08 ± 0.02 μg/mL. Annexin V-FITC/PI double staining showed membrane breakage and nuclei staining. Further, Hoechst 33258 and AO/EtBr stain also confirmed the apoptosis in essential oil-treated HeLa cells.Conclusion:The results obtained suggest that A. monophylla essential oil is a promising natural agent which may be used in preparation of herbal medicine to treat cancer and other diseases.

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“…The 1st and 4th wells were processed without Silymarin. Silymarin concentrations in wells were based on the study by Wang et al (25). Concentrations of 6.25 mg/L were achieved in the 2nd and 5th wells.…”

Section: Preparation Of Placental Samplesmentioning

confidence: 99%

The effect of Silymarin on VEGF, VEGFR-1 and IL-1α levels in placental cultures of severe preeclamptic women

Kaya

Başer

Kaya

et al. 2014

J Turkish German Gynecol Assoc

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Objective:The purpose of this study was to evaluate the effects of Silymarin on vascular endothelial growth factor (VEGF), soluble VEGF Receptor-1 (sVEGFR-1) and Interleukin-1 alpha (IL-1α) levels in placental tissue samples of severely preeclamptic women. Material and Methods:We conducted an in vitro study in Başkent University Faculty of Medicine, Ankara, Turkey between September 2008 and May 2009. A total of 16 placental tissue samples (8 from severe preeclamptic, and 8 from controls) were analysed. Placental samples were incubated, and VEGF, sVEGFR-1, and IL1-α were measured in culture media using an ELISA kit. The effect of Silymarin on these levels was investigated. Descriptive statistics were initially performed, followed by Mann Whitney U-test and Kruskal-Wallis test to compare means between groups. P values less than 0.05 were considered statistically significant. Results:Eight patients were included in the severe preeclampsia (SP) group, whereas the remaining 8 patients were included in the control group. There were no significant correlations between gestational age and placental VEGF, sVEGFR-1 and IL-1α after 48 or 72 hours of incubation. Basal VEGF levels were lower in the SP group; however, it did not reach statistical significance. sVEGFR-1 and IL-1α levels were also similar between the SP and control groups (p>0.05). After 48 and 72 hours of incubation, sVEGFR-1 levels in Silymarin-added SP and control placental cultures were lower than in the samples without Silymarin addition; however, this difference also did not reach significance. Conclusion:Although we could not demonstrate a significant effect on placental cytokines, considering the role of vasospasm, inflammation, angiogenesis, endothelial cell activation, and oxidative stress in preeclampsia, the potential benefits of Silymarin should be evaluated in future trials with a larger sample size. (J Turk Ger Gynecol Assoc 2014; 15: 30-5) Key words: Severe preeclampsia, placenta, vascular endothelial growth factor, Silymarin Received: 17 August, 2013 Accepted: 30 September, 2013 The effect of Silymarin on VEGF, VEGFR-1 and IL-1α levels in placental cultures of severe preeclamptic women After obtaining informed consent, severe preeclamptic women who underwent caesarean section above 30 weeks of gestation were prospectively included in the study. Severe preeclampsia (SP) was defined as new-onset hypertension and proteinuria in a >20 week pregnant woman with one or more of the following features: 1) severely elevated blood pressure (systolic ≥160 mmHg and/or diastolic ≥110 mmHg) measured at least twice with a 6 hour interval; 2) proteinuria ≥5 gr in 24-hour collected urine or ≥2+ in dipstick test; 3) serum creatinine ≥1.2 mg/dL; 4) oliguria (<400 cc in 24 hours); 5) thrombocytopenia (<100,000/mm 3 ); 6) microangiopathic haemolysis (Lactate dehydrogenase (LDH) ≥600 U/L or uncongugated bilirubin ≥1.2 mg/dL); 7) impaired liver function tests (Aspartate serum transaminase ≥ twice normal); 8) foetal growth restriction (FGR); 9) symptoms of cent...

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Protective effects of silybin on human umbilical vein endothelial cell injury induced by H2O2 in vitro

Cited by 33 publications

References 41 publications

Antisense Oligodeoxynucleotide Against Tissue Factor Inhibits Human Umbilical Vein Endothelial Cells Injury Induced by Anoxia-Reoxygenation

Antisense Oligodeoxynucleotide Against Tissue Factor Inhibits Human Umbilical Vein Endothelial Cells Injury Induced by Anoxia-Reoxygenation

Antigenotoxic and apoptotic activities of essential oil of Atalantia monophylla Correa

The effect of Silymarin on VEGF, VEGFR-1 and IL-1α levels in placental cultures of severe preeclamptic women

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