Protective effects of β-glucan as adjuvant combined inactivated Vibrio harveyi vaccine in pearl gentian grouper

Wei, Guangben; Tan, Huiming; Ma, Shaohong; Sun, Guo-Zhong; Zhang, Yilin; Wu, Yuanzhi; Cai, Shuanghu; Huang, Yucong; Jian, Jichang

doi:10.1016/j.fsi.2020.09.027

Cited by 14 publications

(7 citation statements)

References 40 publications

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“…Inactivation of Haemonchus contortus with formalin at an optimal vaccination rate of 1.0×10 8 CFU/fish significantly promoted antibody production in turbot, and enhanced lysozyme activity, total serum protein and antimicrobial properties in the serum of immunized fish ( 153 ). Formalin-inactivated F. harveyi ZJ0603 vaccine (FKC) combined with β-glucan improved immune protection in pearl grouper, and the expression levels of IgM, TNF-α, MHC-Iα, IL-1β and IL-16 in the spleen and the antibody potency, lysozyme and superoxide dismutase activities in the blood of immunized fish were significantly increased ( 154 ). Aeromonas veronii is an extremely important infectious pathogen in carp culture.…”

Section: Classification and Preparation Technology Of Aquatic Vaccinesmentioning

confidence: 99%

Current status and development prospects of aquatic vaccines

Liu

et al. 2022

Front. Immunol.

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Diseases are a significant impediment to aquaculture’s sustainable and healthy growth. The aquaculture industry is suffering significant financial losses as a result of the worsening water quality and increasing frequency of aquatic disease outbreaks caused by the expansion of aquaculture. Drug control, immunoprophylaxis, ecologically integrated control, etc. are the principal control strategies for fish infections. For a long time, the prevention and control of aquatic diseases have mainly relied on the use of various antibiotics and chemical drugs. However, long-term use of chemical inputs not only increases pathogenic bacteria resistance but also damages the fish and aquaculture environments, resulting in drug residues in aquatic products, severely impeding the development of the aquaculture industry. The development and use of aquatic vaccines are the safest and most effective ways to prevent aquatic animal diseases and preserve the health and sustainability of aquaculture. To give references for the development and implementation of aquatic vaccines, this study reviews the development history, types, inoculation techniques, mechanisms of action, development prospects, and challenges encountered with aquatic vaccines.

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Section: Classification and Preparation Technology Of Aquatic Vaccinesmentioning

confidence: 99%

Current status and development prospects of aquatic vaccines

Liu

et al. 2022

Front. Immunol.

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“…At 35 days post-injection, 150 fish from the PBS, GLPs2, GLPs4, GLPs6, and GLPs8 groups (30 fish/group) were intraperitoneally injected with 0.1 ml V. harveyi ZJ0603 at a concentration of 5.6 × 10 8 CFU/ml (Wei et al, 2020b). The mortality of each group was monitored for 14 days after the bacterium challenge.…”

Section: Relative Percent Survival Of Pearl Gentian Grouper After Glpsmentioning

confidence: 99%

Effect of Ganoderma lucidum polysaccharides as immunostimulants against Vibrio harveyi in pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)

Zhang

Zhong²,

Huang

et al. 2022

Front. Mar. Sci.

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Immunostimulants are becoming one of the most effective models for healthy and sustainable development against vibriosis, which is a serious threat to the global aquaculture industry. This study evaluated the effects of different concentrations of Ganoderma lucidum polysaccharides (GLPs) on the immunomodulation and disease resistance of pearl gentian groupers. The relative percent survival of the pearl gentian groupers challenged with Vibrio harveyi ZJ0603 by intraperitoneal injection reached 53.3, 60, 70, and 73.3% in the GLPs2, GLPs4, GLPs6, and GLPs8 groups, respectively, which are significantly higher than that of 33.3% in the control group. Meanwhile, the expression levels of immune-relative genes, including IgM, MHC-Iα, MHC2, IL-1β, and TNF-α, were upregulated in the liver, spleen, head kidney, and thymus within 28 days after GLP injection. The total serum protein and the activities of catalase, superoxide dismutase, and lysozymes in serum were significantly upregulated in all GLP groups compared with those in the control. Moreover, the optimal immunity effect was observed with an injection of GLPs at concentrations of 6 or 8 mg/ml. The results demonstrate that GLPs were an effective immunostimulant, enhancing both specific and non-specific immunity as well as disease resistance in pearl gentian grouper.

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“…According to the method described by Wei et al (2020), liver, spleen, head, and kidney (3 fishes per group) were taken from individual fish in the PBS group and the VAGM001033 mutant group at 42 days post-immunization. The real-time quantitative PCR (qPCR) was performed according to standard protocols with the use of the SYBR Green qPCR Supermix Kit (TransGen, China).…”

Section: Expression Analysis Of Immune-related Gene Of Groupermentioning

confidence: 99%

Characterization of a Diguanylate Cyclase VAGM001033 of Vibrio alginolyticus and Protective Efficacy as a Live Attenuated Vaccine Candidate in Pearl Gentian Grouper

Tan

Lin

et al. 2022

Front. Mar. Sci.

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Vibrio alginolyticus (V. alginolyticus) is one of the important epizootic pathogens in marine animals. VAGM001033 belongs to a diguanylate cyclase, responsible for the synthesis of dimeric guanosine monophosphate (c-di-GMP), a ubiquitous second messenger involved in the function of biofilm formation, motility, and virulence. This study confirmed that VAGM001033 was an active diguanylate cyclase by Congo red assay. The red-stained, dry, and rough form of colonies were observed with the increasing concentration of the L-arabinose on Congo red plates. Also, an in-frame deleted ΔVAGM001033 mutant was constructed and changes of ΔVAGM001033 mutant in physiology and pathogenicity were detected. The ΔVAGM001033 mutant displayed similar morphology and growth curve with the wild-type strain showing no significant differences. The swarming ability of the ΔVAGM001033 mutant was significantly enhanced showing bigger swarming circles, while the biofilm formation, extracellular proteases, and virulence were significantly attenuated. The results of the test for antibiotic susceptibility showed that the wild type and ΔVAGM001033 mutant had similar sensitivity or resistance to most antibiotics used in this study, except cefotaxime and nitrofurantoin. The mutant was sensitive to cefotaxime and nitrofurantoin, while the wild type was intermediate. A total of 756 differentially expressed genes (DEGs) were identified by RNA-seq, of which 109 were upregulated and 647 were downregulated. Flagellar assembly, two-component system, ATP-binding cassette (ABC) transporters, and peptidoglycan biosynthesis were significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Moreover, the ΔVAGM001033 mutant induced high antibody titers and provided immune protectivity with a relative percent survival (RPS) of 82%. Immune-related genes of pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus), namely, IgM, MHC-Iα, interleukin-1β (IL-1β), interleukin-16 (IL-16), and tumor necrosis factor-α (TNF-α) were upregulated after vaccination. Overall, the results suggested that VAGM001033 plays a crucial role in V. alginolyticus. The ΔVAGM001033 mutant might be applied as an effective live vaccine candidate against V. alginolyticus.

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Protective effects of β-glucan as adjuvant combined inactivated Vibrio harveyi vaccine in pearl gentian grouper

Cited by 14 publications

References 40 publications

Current status and development prospects of aquatic vaccines

Current status and development prospects of aquatic vaccines

Effect of Ganoderma lucidum polysaccharides as immunostimulants against Vibrio harveyi in pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)

Characterization of a Diguanylate Cyclase VAGM001033 of Vibrio alginolyticus and Protective Efficacy as a Live Attenuated Vaccine Candidate in Pearl Gentian Grouper

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