Protective immune responses of recombinant VP2 subunit antigen of infectious bursal disease virus in chickens

Pradhan, Satya Narayan; Prince, Prabhu Rajaiah; Madhumathi, Jayaprakasam; Roy, Parimal; Narayanan, R. B.; Antony, Usha

doi:10.1016/j.vetimm.2012.06.019

Cited by 35 publications

(22 citation statements)

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“…Protection against viral infections such as IBDV and ILTV can be achieved by the induction of a high titre of neutralising antibodies as demonstrated using the VP2 antigen for IBDV [30, 31], in contrast to more complicated immune mechanisms necessary to provide protection against other pathogens (eg. Clostridium perfringens [32]).…”

Section: Discussionmentioning

confidence: 99%

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

et al. 2016

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BackgroundEimeria species are parasitic protozoa that cause coccidiosis, an intestinal disease commonly characterised by malabsorption, diarrhoea and haemorrhage that is particularly important in chickens. Vaccination against chicken coccidiosis is effective using wild-type or attenuated live parasite lines. The development of protocols to express foreign proteins in Eimeria species has opened up the possibility of using Eimeria live vaccines to deliver heterologous antigens and function as multivalent vaccine vectors that could protect chickens against a range of pathogens.ResultsIn this study, genetic complementation was used to express immunoprotective virus antigens in Eimeria tenella. Infectious bursal disease virus (IBDV) causes Gumboro, an immunosuppressive disease that affects productivity and can interfere with the efficacy of poultry vaccination programmes. Infectious laryngotracheitis virus (ILTV) causes a highly transmissible respiratory disease for which strong cellular immunity and antibody responses are required for effective vaccination. Genes encoding the VP2 protein from a very virulent strain of IBDV (vvVP2) and glycoprotein I from ILTV (gI) were cloned downstream of 5’Et-Actin or 5’Et-TIF promoter regions in plasmids that also contained a mCitrine fluorescent reporter cassette under control of the 5’Et-MIC1 promoter. The plasmids were introduced by nucleofection into E. tenella sporozoites, which were then used to infect chickens. Progeny oocysts were sorted by FACS and passaged several times in vivo until the proportion of fluorescent parasites in each transgenic population reached ~20 % and the number of transgene copies per parasite genome decreased to < 10. All populations were found to transcribe and express the transgene and induced the generation of low titre, transgene-specific antibodies when used to immunise chickens.ConclusionsE. tenella can express antigens of other poultry pathogens that are successfully recognised by the chicken immune system. Nonetheless, further work has to be done in order to improve the levels of expression for its future use as a multivalent vaccine vector.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1756-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

et al. 2016

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“…Białko kapsydu VP2 ze względu na swoje właściwości indukowania wytwarzania przeciwciał neutralizujących jest głównym przedmiotem zainteresowania w produkcji szczepionek podjednostkowych. Ekspresja białka VP2 powiodła się zarówno w systemie prokariotycznym i eukariotycznym, niemniej w tym ostatnim wydaje się zachowywać lepsze właściwości immunogenne, gdyż tak powstałe białka zachowują naturalną konformację przestrzenną (4,85,105,111). Pewną modyfikacją szczepionki podjednostkowej jest zastosowanie tzw.…”

Section: Badania Nad Nowymi Szczepionkamiunclassified

Selected aspects of infectious bursal disease – the current state of knowledge

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2023

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Infectious bursal disease (IBD) is a highly infectious and contagious immunosuppressive viral disease of chickens with a worldwide economic significance to the poultry industry. Over fifty years have passed since the first confirmed occurrence of the disease, and the virus has spread all over world and evolved into multiple genetic, antigenic and pathotypic variants, becoming a serious threat to the poultry industry. The primary tool in IBD eradication is the maintenance of strict biosecurity in poultry farms and implementation of vaccination programmes which should take into account the current epidemiological knowledge about the IBDV strains circulating in the field. This review article presents the current state of knowledge about the infectious bursal disease virus (IBDV) with special regard to the molecular biology of the virus, immunological aspects, as well as current and future prevention strategies.

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“…Along with these negative effects, endosulfan is immunotoxic, inhibiting the metabolic activity of peripheral blood lymphocytes and splenocytes in chickens (Aggarwal et al, 2008), induces oxidative stress (Sohn et al, 2004, Ayub et al, 2003, El-Shenawy, 2010, Pradhana et al, 2012 and apoptosis (Aggarwal et al, 2003, Kannan et al, 2000.…”

Section: Apoptotic Gene Expression In Endosulfan Exposed Ibd Infectedmentioning

confidence: 99%

Apoptotic Gene Expression in Endosulfan Exposed IBD Infected Birds

Kumar¹,

Prakash²,

Dhama³

et al. 2014

Adv. Anim. Vet. Sci.

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Protective immune responses of recombinant VP2 subunit antigen of infectious bursal disease virus in chickens

Cited by 35 publications

References 27 publications

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

Selected aspects of infectious bursal disease – the current state of knowledge

Apoptotic Gene Expression in Endosulfan Exposed IBD Infected Birds

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