Protective potential of royal jelly against cadmium‐induced infertility in male rats

Cadmium exposure induces nephrotoxicity by mediating oxidative stress, inflammation, and apoptosis. The purpose of this study was to examine the protective effect of royal jelly on Cd-induced nephrotoxicity. Adult male mice were distributed randomly into 4 clusters: untreated, royal jelly-treated (85 mg/kg, oral), CdCl 2 -treated (6.5 mg/kg, intraperitoneal), and pretreated with royal jelly (85 mg/kg) 2 h before CdCl 2 injection (6.5 mg/kg, intraperitoneal) for seven consecutive days. Cd concentration in the renal tissue and absolute kidney weight of the Cd-treated mice were significantly higher than those of control group. The levels of kidney function markers, kidney injury molecules-1 (KIM-1), metallothionein, lipid peroxidation, nitric oxide, tumor necrosis factor-α, interleukin-1β, and the apoptosis regulators Bax and caspases-3 also increased significantly in the renal tissue of Cd-treated mice, whereas the levels of glutathione, antioxidant enzyme activities, and the apoptosis inhibitor Bcl-2 were significantly reduced in the renal tissue of Cd-treated group. Histopathological studies showed vacuolation and congested glomeruli in the kidney tissue of Cd-treated mice. However, all aforementioned Cd-induced changes were attenuated by pretreatment with royal jelly. We therefore concluded that royal jelly attenuated Cd-induced nephrotoxicity and it is suggested that this nephroprotective effect could be linked to its ability to promote the nuclear factor erythroid 2–related factor 2 (Nrf2)/antioxidant responsive element (ARE) pathway.

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“…This finding was in line with that of Ahmed et al . 32 , which revealed that RJ is able to reverse all deleterious effects of Cd.…”

Section: Discussionmentioning

confidence: 97%

Royal jelly attenuates cadmium-induced nephrotoxicity in male mice

Almeer

Albasher

Alarifi

et al. 2019

Sci Rep

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“…afer leaves could be an indication of atrophy or necrosis. Atrophy was reported in testis of cadmium‐intoxicated animals (Ahmed, El‐Shazly, Alkafafy, Mohamed, & Mousa, ; Haouem & El Hani, ), and hypertrophy was reported in organs of some cadmium‐intoxicated animals (Novelli, Vieira, Rodrigues, & Ribas, ; Pari & Murugavel, ).…”

Section: Discussionmentioning

confidence: 99%

High dose of standardised extract ofCostus aferleaves potentiates cadmium reproductive toxicity in Wistar rats

Atere

Akinloye

2019

Andrologia

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Protective effects of standardised extract of Costus afer leaves (CAME), an extract with good antioxidants on cadmium-induced reproductive toxicity in male rats, were investigated in this study. Forty-two adult male Wistar rats were randomly divided into six groups and were treated every day regularly for 4 weeks. G1 (control) rats received 1 ml of vehicle treatment. G2 rats were intoxicated with 2.5 mg kg −1 day −1 s.c cadmium chloride for 1 week. G3 and G4 rats were intoxicated with cadmium as in G2 rats and were treated orally with 100 and 200 mg/kg bwt/day of CAME, respectively, for 4 weeks. Group G5 and G6 rats were orally treated with 100 and 200 mg kg −1 day −1 bwt of CAME, respectively, for 4 weeks. Significant changes (p < 0.05) in andrological parameters (sperm count, sperm morphology, serum testosterone and nitric oxide concentration) and testicular antioxidant parameters (reduced glutathione, lipid peroxidation and activities of catalase, glutathione S-transferase and glutathione peroxidase) caused by Cd toxicity were improved in cadmium-intoxicated rats treated with 100 mg/kg body weight of CAME. Administration of 200 mg/ kg body weight of CAME to cadmium-intoxicated rats potentiated reproductive toxic effects of cadmium. In conclusion, lower dose of CAME is preferred over high dose in treatment of cadmium-induced reproductive toxicity in rats. K E Y W O R D Scadmium, Costus afer, reproductive toxicity, testes

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“…Cd is associated with the development of several health problems, including reproductive dysfunction, renal failure, cancer, cardiovascular diseases, and neurological disorders [2]. Moreover, numerous studies have reported that Cd produces reproductive dysfunctions by disturbing the redox balance, blood-testis barrier, sex hormones homeostasis, sperm count, and enhancing germ cells lose [5,6,7]. Although the precise mechanisms of adverse reactions following Cd exposure are not fully explained, oxidative stress has been reported to play a fundamental role in Cd-induced tissue injuries [8].…”

Section: Introductionmentioning

confidence: 99%

Royal Jelly Abrogates Cadmium-Induced Oxidative Challenge in Mouse Testes: Involvement of the Nrf2 Pathway

Almeer

Soliman

Kassab

et al. 2018

IJMS

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The current study examined the efficacy of royal jelly (RJ) against cadmium chloride (CdCl2)-induced testicular dysfunction. A total of 28 Swiss male mice were allocated into four groups (n = 7), and are listed as follows: (1) the control group, who was intraperitoneally injected with physiological saline (0.9% NaCl) for 7 days; (2) the RJ group, who was orally supplemented with RJ (85 mg/kg daily equivalent to 250 mg crude RJ) for 7 days; (3) the CdCl2 group, who was intraperitoneally injected with 6.5 mg/kg for 7 days; and (4) the fourth group, who was supplemented with RJ 1 h before CdCl2 injection for 7 days. Cd-intoxicated mice exhibited a decrease in serum testosterone, luteinizing hormone (LH), and follicle stimulating hormone (FSH) levels. A disturbance in the redox status in the testicular tissue was recorded, as presented by the increase in lipid peroxidation and nitrate/nitrite levels and glutathione (GSH) depletion. Moreover, the activities of glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), and nuclear factor (erythroid-derived 2)-like-2 factor (Nrf2) and their gene expression were inhibited. In addition, interleukin-1ß (IL-1β) and tumor necrosis factor-α (TNF-α) levels were elevated. Furthermore, Cd triggered an apoptotic cascade via upregulation of caspase-3 and Bax and downregulation of Bcl-2. Histopathological examination showed degenerative changes in spermatogenic cells, detachment of the spermatogenic epithelium from the basement membrane, and vacuolated seminiferous tubules. Decreased cell proliferation was reflected by a decrease in proliferating cell nuclear antigen (PCNA) expression. Interestingly, RJ supplementation markedly minimized the biochemical and molecular histopathological changes in testes tissue in response to Cd exposure. The beneficial effects of RJ could be attributed to its antioxidative properties.

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Protective potential of royal jelly against cadmium‐induced infertility in male rats

Cited by 19 publications

References 60 publications

Royal jelly attenuates cadmium-induced nephrotoxicity in male mice

Royal jelly attenuates cadmium-induced nephrotoxicity in male mice

High dose of standardised extract ofCostus aferleaves potentiates cadmium reproductive toxicity in Wistar rats

Royal Jelly Abrogates Cadmium-Induced Oxidative Challenge in Mouse Testes: Involvement of the Nrf2 Pathway

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