Protective role of dichloromethane extract of solanum dasyphyllumin in gentamicin induced nephrotoxicity in mice

Abstract

Gentamicin belong to the aminoglycoside antibiotic, which has a broad spectrum activities against gram positive and gram negative bacteria, but most effective in the treatment of gram negative bacteria. Gentamicin can withstand ABSTRACT This study investigated the effect of dichloromethane extract of Solanum dasyphyllum on gentamicin-induced nephrotoxicity in mice. Thirty mice were randomly divided into six groups (Groups 1-6, 5 each). Nephrotoxicity was induced using 100 mg/kg gentamicin administered intraperitoneally with concomitant administration of different doses of dichloromethane Solanum dasyphyllum extract (SdDMF) (100, 200, and 400mg/kg respectively) orally for 8 days. After the treatment, blood samples were obtained, allowed to clot, and centrifuged to obtain the serum which was used to determine urea, creatinine, and blood urea nitrogen (BUN). Kidney tissue malondialdehyde (MDA) and glutathione (GSH) concentrations; and catalase (CAT) activities were also determined. Result revealed a decrease in tissue MDA, serum creatinine, urea, and BUN in the extract-treated groups. Also, increased tissue CAT activities and GSH concentrations were observed in the extract-treated groups. In conclusion, this study showed that SdDMF can be reno-protective and effectively inhibit gentamicin-induced nephrotoxicity.

Neutralizing Effect of Solanum dasyphyllum Schumach. &Thonn Extract against Naja nigricollis Venom-induced Toxicity

“…The plant is branched at the base, with branches heavily armed with 2-7 mm long prickles. S. dasyphyllum is used ethnomedically to treat poisoning [16].The plant is reported to possess anticonvulsant, antioxidant, and neuromuscular properties [17]. In the southwestern part of Nigeria, the fruit of S. dasyphyllum is commonly mixed with local black soap and applied to incisions at snakebite sites.…”