Protective role of ginseng in endometriosis during covid-19

Song, Jae Bok; Lee, Young‐Joo

doi:10.1016/j.jgr.2022.08.002

Cited by 6 publications

(1 citation statement)

References 71 publications

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“…It has the characteristics of implantation, infiltration and high recurrence rate, because it has several biological behaviors similar to malignant lesions, including cell invasion, neo-angiogenesis, and decreased apoptosis [ 1 , 2 ]. Endometriosis (EMs) is a common gynecologic disease with an overall prevalence of about 10–15%, of which most patients are detected in the reproductive age and the mechanisms of which have not been fully elucidated [ 3 ]. Endometriosis not only causes pain, but also has serious consequences such as infertility [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Roles of increased NUCKS1 expression in endometriosis

Li¹,

Chen

Wang³

et al. 2023

BMC Women's Health

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Background Endometriosis is still a difficult problem for women. The Nuclear Ubiquitous Casein and cyclin-dependent Kinase Substrate 1 (NUCKS1) gene is located on human chromosome 1q32.1. It encodes the NUCKS1 protein, a 27 kDa nuclear DNA binding protein that plays an important role in cell growth and proliferation. NUCKS1 plays an important role in the development of many diseases. However, its role in endometriosis is unclear. Methods Ectopic endometrial tissues and normal tissue specimens were collected, and the expression of NUCKS1, NF-κB and PI3K was detected by RT-qPCR and immunohistochemistry. Inhibition of NUCKS1 in hEM15A cells, study the changes in cell viability, apoptosis, migration and protein expression by CCK8 assay, flow cytometry, wound-healing assay, western blot and ELISA techniques. The comparison of differences between the two groups was implemented using unpaired sample t test or Mann-whitney U test. One-way analysis of variance or Kruskal-wallis test was used for comparisons among the three groups. Results (1) NUCKS1 is highly expressed in endometriosis tissues. (2) Inhibition of NUCKS1 decreases cell viability and capability of migration, and increases apoptosis in endometriosis cells. (3) Expressions of NF-κB and PI3K are increased in endometriosis tissues, and inhibition of NUCKS1 decreases the expression levels of PI3K and NF-κB in endometriosis cells. (4) Inhibition of NUCKS1 decreases the expression of VEGF. Conclusion (1) NUCKS1 is overexpressed in endometriosis, and inhibition of NUCKS1 inhibits cell viability and capability of migration, and increases apoptosis. (2) NUCKS1 promotes the progress of endometriosis through activating PI3K and NF-κB pathways, and VEFG is also involved in this process.

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