Protective role of humic acids against dicamba-induced genotoxicity and DNA methylation in<i>Phaseolus vulgaris</i>L.

Yıldırım, Nalan; Ağar, Güleray; Taspinar, Mahmut Sinan; Turan, Metin; Aydın, Murat; Arslan, Esra

doi:10.1080/09064710.2014.891650

Cited by 13 publications

(12 citation statements)

References 38 publications

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“…In our study, DNA hypermethylation (2,4-D and picloram) and hypomethylation (dicamba) were detected. Similarly, Yildirim et al [15] reported that dicamba caused DNA hypomethylation in Phaseolus Figure 1. RAPD bands amplified by the OPA-4 primer in picloram treatments.…”

Section: Resultsmentioning

confidence: 87%

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Analyses of somaclonal variation in endosperm-supported mature embryo culture of rye (Secale cereale L.)

Aydın

Arslan

Taspinar

et al. 2016

Biotechnology & Biotechnological Equipment

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Genetic variations, and especially somaclonal variations, are undesirable in genetic transformation. In this study, random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) markers were used for detection of the variation in calli that were obtained from endosperm-supported mature embryo of rye on Murashige and Skoog (MS) medium containing different auxins (2,4-D, dicamba and picloram) at a range of different concentrations (2, 4, 6, 8, 10 and 12 mg/L). High level of auxins caused a reduction in the genomic template stability (GTS) value. While the highest GTS was observed in the calli maintained on MS medium with 2 mg/L dicamba (98.4%), the calli maintained on MS medium with 12 mg/L picloram were found to show the least GTS (81.7%) when RAPD patterns were analysed. Epigenetic changes were more frequent and variable than genetic changes when compared to RAPD and CRED-RA results. DNA hypermethylation was observed at higher concentrations of 2,4-D and picloram, whereas DNA hypomethylation was observed in dicamba. These results indicate that RAPD and CRED-RA techniques can be used for detection of somaclonal variation in in vitro cultures, which is a fundamental step in plant genetic transformation.

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Section: Resultsmentioning

confidence: 87%

“…The average number of polymorphisms (%) was calculated for each dose to realize CRED-RA analysis. To calculate the polymorphisms (%), the following formula was used: polymorphisms (%) D 100 £ a/n [15].…”

Section: Discussionmentioning

confidence: 99%

Analyses of somaclonal variation in endosperm-supported mature embryo culture of rye (Secale cereale L.)

Aydın

Arslan

Taspinar

et al. 2016

Biotechnology & Biotechnological Equipment

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“…The average number of polymorphisms (%) was calculated for each dose to realize CRED-RA analysis. To calculate the number of polymorphisms (%), the following formula was used 100x a/n (Yildirim et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Protective Role of Putrescine against Picloram Induced Genomic Instability and DNA Methylation in Phaseolus vulgaris

Taspinar¹,

Arslan²,

Aydın³

et al. 2017

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Picloram (4-amino-3-5-6-trichloropicolinic acid) is an important synthetic auxin and it is one of the most widely used herbicides in agriculture. The use of picloram is representing a potential hazardous to ecosystems and the human health. Putrescine is a kind of polyamine which has a role in plant metabolism such as protecting membrane stability, removing free radicals and nucleic acid and protein synthesis, etc. In this study, DNA damage levels in Phaseolus vulgaris treated with picloram, DNA methylation changes and whether putrescine was any alleviative impact on these alterations were investigated. DNA methylation pattern changes and DNA damage levels were determined by using Randomly Amplified Polymorphic DNA (RAPDs) and Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RAs), respectively. The obtained results indicated that all doses of picloram (5, 10, 20 and 40 mg L -1 ) had a negative effect on RAPDs profile changes (increased DNA damage levels) and decreased of Genomic Template Stability (GTS) and also DNA hypomethylation was seen. However, all concentrations of putrescine (0.01, 0.1 and 1 ppm) reduced these harmful effects of picloram. Consequently, putrescine can be an alternative for reducing genotoxic damage against chemical mutagens in plants.Keywords: DNA methylation, genomic instability, picloram, putrescine ÖZET: Pikloram (4-amino-3-5-6-trikloropikolinik asit) önemli bir sentetik oksin olup, tarımda en çok kullanılan herbisitlerden biridir. Pikloram kullanımı, ekosistem ve insan sağlığı için potansiyel bir tehlike oluşturmaktadır. Bir poliamin çeşidi olan putresinin bitki metabolizmasında; membran stabilitesinin korunması, serbest radikallerin uzaklaştırılması ve nükleik asit ve protein sentezi gibi rolü vardır. Bu çalışma, pikloram uygulanan Phaseolus vulgaris' teki DNA hasar düzeyleri ve DNA metilasyon değişiklikleri ile putresinin bu değişimler üzerinde koruyucu etkisinin olup olmadığının belirlenmesini amaçlamıştır. DNA hasar düzeylerini ve DNA metilasyon değişimlerini belirlemek için Rastgele Çoğaltılmış Polimorfik DNA (RAPD) ve Çift Restriksiyon Enzim KesimiRastgele Çoğaltım (CRED-RA) kullanılmıştır. Sonuçlar, tüm pikloram dozlarının (5, 10, 20 ve 40 mg L -1 ) RAPD profil değişikliklerini (DNA hasarının) artırdığını ve Genomik Kararlılık Stabilitesini (GTS) azalttığını ve ayrıca DNA hipometilasyonunun oluştuğunu göstermiştir. Bununla birlikte, kullanılan tüm putresin konsantrasyonları (0.01, 0.1 ve 1 ppm) putresinin bu zararlı etkilerini azaltmıştır. Sonuç olarak, putresin, bitkilerdeki kimyasal mutajenlere karşı genotoksik hasarı azaltmak için bir alternatif olabilir.

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“…RAPD and CRED-RA patterns were analyzed by using the Total Lab (TL120) computer software (Yildirim et al 2014). …”

Section: Methodsmentioning

confidence: 99%

Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome

Taspinar¹,

Sığmaz²,

Aydın³

et al. 2018

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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2,4-Dichlorophenoxyacetic acid (2,4-D) is one of the several herbicides that widely used to prevent development of Taraxacum officinale and broadleaf weeds on cereal crops culture. However, it is known that 2,4-D can cause genetic damage to plants at low concentrations as well as produce observable physiological effects. There is no report investigating the effect of mammalian hormones in crops against the applied 2,4-D. Therefore, the present study was aimed at investigating levels of DNA damage, changes in DNA methylation and DNA stability in common bean (Phaseolus vulgaris) exposed to 2,4-D and determine whether β-estradiol has any effect. RAPDs (Randomly Amplified Polymorphic DNA) and CRED-RAs (Coupled Restriction Enzyme Digestion-Random Amplification) techniques were used to define the DNA damage levels and changes in the pattern of DNA methylation. The obtained data demonstrated that 2,4-D led to an increase in RAPDs profile changes (DNA damage), and a reduction in genomic template stability (GTS). The effects caused by 2,4-D were decreased after application with different concentrations of β-estradiol. The results of this study clearly show that β-estradiol could be used function effectively to prevented from the genetic and epigenetic changes caused by 2,4-D herbicide in common bean.

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Protective role of humic acids against dicamba-induced genotoxicity and DNA methylation inPhaseolus vulgarisL.

Cited by 13 publications

References 38 publications

Analyses of somaclonal variation in endosperm-supported mature embryo culture of rye (Secale cereale L.)

Analyses of somaclonal variation in endosperm-supported mature embryo culture of rye (Secale cereale L.)

Protective Role of Putrescine against Picloram Induced Genomic Instability and DNA Methylation in Phaseolus vulgaris

Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome

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