Protective Role of Hypothermia Against Heat Stress in Differentiated and Undifferentiated Human Neural Precursor Cells: A Differential Approach for the Treatment of Traumatic Brain Injury

Vishwakarma, Sandeep Kumar; Bardia, Avinash; Fathima, Nusrath; Lakkireddy, Chandrakala; Syed, Rabbani; Raju, Nagarajan; Srinivas, G.; Raj, Avinash; Annamaneni, Sandhya; Satti, Vishnupriya; Tiwari, Santosh Kumar; Paspala, Syed Ameer Basha; Khan, Aleem Ahmed

doi:10.29252/nirp.bcn.8.6.453

Cited by 4 publications

(3 citation statements)

References 37 publications

(35 reference statements)

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“…It should be noted that a mild hyperthermia (38.5°C) increased NSCs/NPCs proliferation and neuronal differentiation through an initial and brief increase in Hsp70 followed by the more sustained increase in Hsp27, whereas no significant effect was reported on Hsp90 expression (26). In contrast, a mild hypothermia after a heat stress decreases Hsp70 expression in NPCs, as reported during the differentiation of NSCs/NPCs in brain cells (27).…”

Section: Hsp Expression At the Bbb Levelmentioning

confidence: 81%

Molecular chaperones in the brain endothelial barrier: neurotoxicity or neuroprotection?

Thüringer

Garrido

2019

The FASEB Journal

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Brain microvascular endothelial cells (BMECs) interact with astrocytes and pericytes to form the blood–brain barrier (BBB). Their compromised function alters the BBB integrity, which is associated with early events in the pathogenesis of cancer, neurodegenerative diseases, and epilepsy. Interestingly, these conditions also induce the expression of heat shock proteins (HSPs). Here we review the contribution of major HSP families to BMEC and BBB function. Although investigators mainly report protective effects of HSPs in brain, contrasted results were obtained in BMEC, which depend both on the HSP and on its location, intra‐ or extracellular. The therapeutic potential of HSPs must be scrupulously analyzed before targeting them in patients to reduce the progression of brain lesions and improve neurologic outcomes in the long term.—Thuringer, D., Garrido, C. Molecular chaperones in the brain endothelial barrier: neurotoxicity or neuroprotection? FASEB J. 33, 11629–11639 (2019). http://www.fasebj.org

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Section: Hsp Expression At the Bbb Levelmentioning

confidence: 81%

Molecular chaperones in the brain endothelial barrier: neurotoxicity or neuroprotection?

Thüringer

Garrido

2019

The FASEB Journal

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“…Kūno temperatūros sumažėjimas 1 0 C sumažina smegenų metabolizmą 5 procentais [10]. Šis procesas iš dalies apsaugo ląsteles nuo hipoksijos sukeltos depoliarizacijos [11]. Sumažėjęs metabolizmas nėra vienintelė neuroprotekcijos priežastis.…”

Section: Terapinės Hipotermijos Istorija Ir Praktinis Taikymasunclassified

Terapinės Hipotermijos Istorija Ir Praktinis Taikymas

Badaras,

Šerpytis

2022

Health Sciences

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Terapinė hipotermija – kūno šaldymas iki žemesnės nei fiziologinė temperatūros. Šis gydymo metodas taikomas siekiant pagerinti asistoliją patyrusių pacientų neurologines baigtis. Šiuo metu nėra sukurto tikslaus ir konkretaus terapinės hipotermijos protokolo. Šiame straipsnyje apžvelgiame šios terapinės procedūros istoriją, fiziologinius veikimo mechanizmus ir praktinio taikymo klausimus. Nėra aiškaus sutarimo, kokią tikslinę temperatūrą reikėtų pasirinkti, kada pradėti taikyti hipotermiją, kiek laiko tęsti gydymą. Naujų tyrimų du o m e n y s k v e s t i o n u o j a t e rapinės hipotermijos naudą, palyginus su aktyviu normotermijos palaikymu. Europos gaivinimo taryba 2021 metų gairėse terapinę hipotermiją rekomenduoja visiems pacientams, patyrusiems širdies sustojimą, nepriklausomai nuo to, kur sustojo širdis, ar koks buvo širdies ritmas prieš jai sustojant.

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“…Previous studies have shown that exposure of NSCs to high temperatures during the proliferation phase in the early development arrested cell proliferation and induced cell death in cultured guinea pig [ 11 – 13 ], rat [ 14 ], and mouse embryos in vivo [ 15 ]. Moreover, severe heat stress exposure of NPCs reduced the cell viability of neurospheres [ 16 ], whereas mild heat exposure increased NS/PC proliferation in heat-acclimated rats [ 17 ]. These findings suggest that exposure to HS affects the survival and proliferation of NSCs and NS/PCs, both in vivo and in vitro .…”

Section: Introductionmentioning

confidence: 99%

Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells

Omori

Otsu²,

Nogami

et al. 2021

PLoS ONE

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Cells have a regulatory mechanism known as heat shock (HS) response, which induces the expression of HS genes and proteins in response to heat and other cellular stresses. Exposure to moderate HS results in beneficial effects, such as thermotolerance and promotes survival, whereas excessive HS causes cell death. The effect of HS on cells depends on both exogenous factors, including the temperature and duration of heat application, and endogenous factors, such as the degree of cell differentiation. Neural stem cells (NSCs) can self-renew and differentiate into neurons and glial cells, but the changes in the HS response of symmetrically proliferating NSCs in culture are unclear. We evaluated the HS response of homogeneous proliferating NSCs derived from mouse embryonic stem cells during the proliferative phase and its effect on survival and cell death in vitro. The number of adherent cells and the expression ratios of HS protein (Hsp)40 and Hsp70 genes after exposure to HS for 20 min at temperatures above 43°C significantly increased with the extension of the culture period before exposure to HS. In contrast, caspase activity was significantly decreased by extension of the culture period before exposure to HS and suppressed the decrease in cell viability. These results suggest that the culture period before HS remarkably affects the HS response, influencing the expression of HS genes and cell survival of proliferating NSCs in culture.

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Protective Role of Hypothermia Against Heat Stress in Differentiated and Undifferentiated Human Neural Precursor Cells: A Differential Approach for the Treatment of Traumatic Brain Injury

Cited by 4 publications

References 37 publications

Molecular chaperones in the brain endothelial barrier: neurotoxicity or neuroprotection?

Molecular chaperones in the brain endothelial barrier: neurotoxicity or neuroprotection?

Terapinės Hipotermijos Istorija Ir Praktinis Taikymas

Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells

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