Protective Role of Nigerian Honey on Sperm indices and Testis in Sucrose-Fed Rats

Oyelowo, Oluwakemi; Adekunbi, Daniel; Dada, Kayode

doi:10.3329/bjms.v13i2.18301

Cited by 11 publications

(6 citation statements)

References 12 publications

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“…It regained the normal appearance of the epididymal structure with a highly increase in the number of sperm inside its tubules. While Mohamed et al, (2012) revealed that no significant change in the epididymal weight was induced by honey consumption, Abdul-Ghani et al (2008) and Oyelowo et al (2014) reported that honey supplementation to rats increased epididymal weight significantly. To the best of our knowledge, no previous study has been undertaken to investigate the beneficial effects of honey on the epididymal histology.…”

Section: Discussionmentioning

confidence: 98%

Trihoney ameliorates hypercholesterolemia-induced epididymal histopathological changes in male rabbits

Mohamed

Ibrahim

Alfarisi

et al. 2020

APJMBB

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Hypercholesterolemia has been linked to weight change and histopathological alteration of male reproductive organs. The epididymis was suggested to be an early target of lipid-related infertility and can be dramatically affected by excess intake of a high cholesterol diet. On the other hand, the interest has been increased towards the use of honey as a prophylactic and therapeutic agent for various diseases. Therefore, the purpose of this study is to investigate the effects of Trihoney (a mixture of Trigona, Mellifera and Tualang) on epididymal weight change and histopathological alterations in hypercholesterolemic male rabbits and compare its effects with atorvastatin. Forty-eight mature male New Zealand white rabbits were divided into 6 groups. Two groups received standard rabbit pellet with 0 and 0.6 g/kg/day of Trihoney respectively while the other four groups received 1% cholesterol diet with 0, 0.3, 0.6 g/kg/day of Trihoney, and 2 mg/kg/day of atorvastatin. After 12 weeks, the rabbits were sacrificed and the epididymides were harvested for evaluation of weight and histopathological changes. Administration of 1% cholesterol diet either alone or in combination with atorvastatin caused a significant reduction in the epididymal weight and epididymal atrophy. Supplementation of Trihoney particularly at the dose of 0.6 g/kg/day improved epididymal weight, regained the normal architecture of the epididymal histology and increased the number of mature sperm inside the tubules of the epididymis. Based on these results, Trihoney exhibited its potential health benefit as a protective agent against epididymal weight reduction and histopathological alterations in hypercholesterolemic rabbits.

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Section: Discussionmentioning

confidence: 98%

Trihoney ameliorates hypercholesterolemia-induced epididymal histopathological changes in male rabbits

Mohamed

Ibrahim

Alfarisi

et al. 2020

APJMBB

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“…These findings were supported by numerous studies in which administration of different types of honey to normal and diabetic rats exhibited no negative impact on the testicular weights (Michael et al, 2015;Mohamed et al, 2012;Syazana et al, 2011). Moreover, Oyelowo et al (2014) reported that honey supplementation to rats increased testicular weight significantly. The improvement of testicular weight via Trihoney administration could be through the enhancement of spermiogenesis and spermatogenesis by maintaining testicular integrity and optimizing neuroendocrine gonadal axis with an elevation of testosterone hormone and a subsequent improvement in spermatogenesis as shown previously with other types of honey (Abdul-Ghani et al, 2008;Kenani et al, 2015;Michael et al, 2015;Mohamed et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Trihoney improves testicular weight change and histopathological alterations in hypercholesterolemic rabbits

Mohamed

Alfarisi

Wahab

et al. 2020

APJMBB

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Histopathological examination of testicular tissue is the most reliable and sensitive method for detecting effects on spermatogenesis. Hypercholesterolemia reduces testicular weight, induces testicular degenerative changes, impairs spermatogenesis, affects Leydig and Sertoli cells and induces inflammation and fibrosis of testicular tissue. Based on numerous studies, honey has the ability to improve testicular histopathological abnormalities. To date, whether honey has any protective role against the effects of hypercholesterolemia on male reproductive functions is yet to be explored. This study investigated the effects of Trihoney (a mixture of Trigona, Mellifera and Tualang honeys) on changes in testicular weight and histopathological alterations induced by hypercholesterolemia in male New Zealand white rabbits. These changes were compared with the effects of atorvastatin (a lipid lowering agent) based on the same parameters. Forty-eight male New Zealand white rabbits were assigned into 6 groups and received different diets as follows; Control: commercial pellet; CH: commercial pellet and 0.6 g/kg/day Trihoney; HCD: 1% cholesterol diet; DH1: 1% cholesterol diet and 0.3 g/kg/day Trihoney; DH2: 1% cholesterol diet and 0.6 g/kg/day Trihoney; DAt: 1% cholesterol diet and 2 mg/kg/day atorvastatin. After 12 weeks, blood samples were collected for lipid analysis, the rabbits were sacrificed and the testes were harvested to evaluate any weight and histopathological changes. Administration of 1% cholesterol diet either alone or in combination with atorvastatin caused a significant reduction in the testicular weight, testicular tubular degenerative changes and spermatogenesis impairment. Trihoney, particularly, at the dose of 0.6 g/kg/day improved testicular weight, ameliorated the testicular tubular degenerative changes and enhanced spermatogenesis. The findings of this study suggest that Trihoney plays a favourable role in the protection against testicular weight reduction and histopathological changes induced by hypercholesterolemia. On the other hand, atorvastatin per se may have toxic effects on testicular tissue.

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“…The effect of honey on male reproductive hormones has been investigated in various studies but this is the first study attempts to determine the effect of honey on male reproductive hormonal disturbances induced by hypercholesterolaemia. Oyelowo et al (2014) 9 reported that consumption of honey increased testosterone and reduced FSH and LH in rats fed with normal diet and rats received high sucrose solution. The beneficial effect of honey in increasing low serum testosterone, and reducing elevated serum FSH and LH was detected in noise-exposed rats.…”

Section: Discussionmentioning

confidence: 99%

“…4,6,7 On the other hand, the use of honey in previous studies resulted in an improvement of the male reproductive hormonal disturbances through increasing testosterone and reducing FSH and LH in rats fed with high sucrose diet and rats exposed to noise stress. 8,9 Trihoney is a product developed by the Department of Nutrition Sciences of Kulliyyah of Allied Health Sciences of International Islamic University Malaysia. It is a mixture of Trigona, Mellifera and Tualang honey at a ratio optimized by Response Surface Methodology of Design Expert Version 6.0 software.…”

Section: Introductionmentioning

confidence: 99%

Effect of Trihoney (A Mixture of Trigona, Mellifera and Tualang) on Male Reproductive Hormones and Insulin Resistance in Hypercholesterolaemic Rabbits

Mohamed¹,

Ibrahim²,

Alfarisi³

et al. 2020

imjm

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Introduction: Hypercholesterolaemia affects Sertoli and Leydig cells secretory functions, impairs steroidhormone biosynthesis, and disrupts the hypothalamic-pituitary-gonadal axis. The use of honey in previousstudies resulted in an improvement of male reproductive hormonal disturbances. This study aimed toinvestigate the protective effects of Trihoney on hypercholesterolemia-induced male reproductive hormonalchanges in male rabbits and compare its effects with atorvastatin. Materials and methods: Forty-eight maleNew Zealand white rabbits were assigned into 6 groups as follows; Control: commercial pellet; CH:commercial pellet with 0.6 g/kg/day Trihoney; HCD: 1% cholesterol diet; DH1: 1% cholesterol diet with 0.3g/kg/day Trihoney; DH2: 1% cholesterol diet with 0.6 g/kg/day Trihoney and DAt: 1% cholesterol diet with2mg/kg/day atorvastatin. After 12 weeks, the rabbits were sacrificed and blood samples were collected foranalysis of hormones and pro-inflammatory cytokines and calculation of HOMA-IR. The testes werehomogenized for intra-testicular testosterone measurement. Results: Serum testosterone reducedsignificantly in HCD (p<0.05) and DAt (p<0.05) groups. Likewise, intra-testicular testosterone reducedsignificantly in HCD (p<0.01) and DAt (p<0.01) groups. Serum FSH increased significantly in HCD (p<0.001)and DAt (p<0.01). Trihoney particularly at the dose of 0.6 g/kg/day improved serum and intra-testiculartestosterone (p<0.05) and FSH (p<0.05). Trihoney and atorvastatin improved serum pro-inflammatorycytokines. Trihoney and atorvastatin did not affect HOMA-IR. Conclusion: Trihoney attenuated thedetrimental effects of hypercholesterolaemia on male reproductive hormones which probably through alocal effect on testicular tissue and Trihoney anti-inflammatory effect independent of insulin resistance.Atorvastatin did not counteract the impact of hypercholesterolaemia on the reproductive hormones.

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Protective Role of Nigerian Honey on Sperm indices and Testis in Sucrose-Fed Rats

Cited by 11 publications

References 12 publications

Trihoney ameliorates hypercholesterolemia-induced epididymal histopathological changes in male rabbits

Trihoney ameliorates hypercholesterolemia-induced epididymal histopathological changes in male rabbits

Trihoney improves testicular weight change and histopathological alterations in hypercholesterolemic rabbits

Effect of Trihoney (A Mixture of Trigona, Mellifera and Tualang) on Male Reproductive Hormones and Insulin Resistance in Hypercholesterolaemic Rabbits

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