Protective role of polyphenols fromBauhinia hookeriagainst carbon tetrachloride-induced hepato- and nephrotoxicity in mice

Abstract

(2015) Protective role of polyphenols from Bauhiniahookeri against carbon tetrachloride-induced hepato-and nephrotoxicity in mice, Renal Failure, 37:7, 1198Failure, 37:7, -1207 AbstractThe hepatoprotective and nephroprotective activity of a polyphenol-rich fraction (BHPF) obtained from Bauhinia hookeri was investigated against CCl 4 -induced acute hepatorenal toxicity in mice. BHPF was administered (100, 200 and 400 mg/kg/day) for 5 days, then CCl 4 was administered. BHPF pretreatment significantly (p50.001) inhibited the CCl 4 -induced increase in ALT, AST, ALP, LDH, total bilirubin, cholesterol, creatinine, uric acid, urea and malondialdehyde in a dose-dependent manner. In contrast, BHPF pretreatment markedly increased the contents of glutathione and superoxide dismutase in the liver and kidney tissues, indicating the strong in vivo antioxidant activity of BHPF. Pretreatment with BHPF preserved the hepatic architecture and conferred marked protection against necrosis and ballooning degeneration. Pretreatment with BHPF reduced the inflammatory cell aggregation and degenerative changes in the lining epithelium of the kidney tubules. It can be concluded that BHPF has a remarkable hepato-and nephroprotective activity by enhancing the antioxidant defense status, reducing lipid peroxidation and protecting against the histopathological changes induced by CCl 4 in the liver and kidney tissues.

Hepato-protective activity of three different extracts of Butea monosperma in CCl4 and Isoniazid induced toxicity in wistar rats

“…In ALP assay the blue color developed which was read at 510 nm against blank and the activity is expressed as IU/L of serum. To estimate the TBil readings were taken at 540 nm, the level of total bilirubin was expressed as mg/ dl of serum [9].…”

“…In a number of scientific studies high free radical exposure have been reported to induce membrane lipid peroxidation and protein oxidation as well as deleterious effects on enzymatic system of the cells [6][7][8]. In both of the above mentioned treatments, the serum values of alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT) and total biliruin (TBil) were found to be abnormally increased, hence are often used as biochemical markers of hepatic injury [9].…”

“…Antioxidants are well known protective agents which either inhibit generation of free radicals or by enhance their removal [7,9]. Thousands of herbs have been reported to have antioxidative property [6,[10][11][12].…”

“…BM is known source of potent antioxidants such as quercetin, flavianoids, tannins, triterpenoid, gallic acid, ellagic acid, resin etc. In different premedical and medical anlaysis different parts of this plant has been known to show anti-diabetic, antibacterial and the inflammatory action [1,9,14]. But available reports also showed differential potencies of herbs in different extraction medium.…”

“…Enzymatic activity such as ALP, AST and ALT as well as serum total bilirubin (TBil) were taken as studied parameters. In both experiments, silymarin a known hepato-protective drug [9] was used as standard drug. Tissue histology was also performed to authenticate the results.…”