Protective role of Punica granatum (pomegranate) peel and seed oil extracts on diethylnitrosamine and phenobarbital-induced hepatic injury in male rats

Shaban, Nadia Z.; Elkersh, Mohamed A.; El‐Rashidy, Fatma H.; Habashy, Noha H.

doi:10.1016/j.foodchem.2013.04.134

Cited by 90 publications

(66 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Schubert et al (1999) demonstrated that PSO has antioxidant activity higher than red wine, green tea and butylated hydroxyanisole. Shaban et al (2013) showed that PSO presents low phenolic and flavonoid content, indicating that the antioxidant property is due, in addition to these compounds, to the presence of other substances as triterpenoids, c-tocopherol, 17-estradiol, estrogens ''estrone and estriol '', testosterone, b-sitosterol, coumesterol, campesterol, stigmasterol, punicic acid and flavonoids ''genistein and daidzein'' (Lansky & Newman 2007;Kaufman & Wiesman 2007).…”

Section: Discussionmentioning

confidence: 99%

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Ferreira

Gehrcke

Cervi

et al. 2016

Pharmaceutical Biology

View full text Add to dashboard Cite

(2016) Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells, Pharmaceutical Biology, 54:12, 2968-2977, DOI: 10.1080/13880209.2016 Context: Glioma is a malignant brain tumor with rapid proliferation, infiltrative growth, poor prognosis and it is chemoresistent. Pomegranate seed oil (PSO) has antioxidant, anti-inflammatory and antitumor properties. This study showed the optimization of PSO nanoemulsions (NEs) as an alternative for glioma treatment.Objective: The study aimed to evaluate PSO NEs cytotoxicity on human blood cells and antiglioma effects against C6 cells. Materials and methods: NEs were prepared by the spontaneous emulsification method, using PSO at 1.5 and 3.0%, and were evaluated regarding their physical stability and antioxidant activity. Toxicity evaluations in human blood cells were performed in terms of cell viability, genotoxicity, lipid peroxidation, protein carbonylation, catalase activity and hemolysis at 0.1, 0.25 and 0.5 mg/mL PSO, after a 72-h incubation period. In vitro antitumor effect was determined against glioma cells after 24 and 48 h, and astrocytes were used as a non-transformed cell model. Results: Formulations presented droplet size below 250 nm, low polydispersity index, negative zeta potential and pH in the acid range. NEs and PSO had scavenging capacity around 30% and promoted a proliferative effect in mononuclear cells, increasing about 50% cell viability. No genotoxic and oxidative damage was observed in lipid peroxidation, protein carbonylation and catalase activity evaluations for NEs. Hemolysis study showed a hemolytic effect at high concentrations. Moreover, formulations reduced only tumor cell viability to 47%, approximately. Discussion and conclusion: Formulations are adequate and safe for intravenous administration. Besides, in vitro antitumor activity indicates that NEs are promising for glioma treatment.ARTICLE HISTORY

show abstract

Section: Discussionmentioning

confidence: 99%

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Ferreira

Gehrcke

Cervi

et al. 2016

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…The initiation of LPO starts the production of toxic radicals such as MDA, hydrogen peroxide, F2-isoprostanes, hydroxyl radicals, and 4-hydroxynonenal, which causes cell death, cell membrane damage, cellular function, oxidative stress, and carcinogenesis. 35,36 The content of MDA and CD was increased during the HCC development from increased production of free radicals such as alkoxy and peroxyl radicals, and enhanced the deposition of these radicals to cause the tissue toxicity and dysfunction of endogenous antioxidant systems. The results revealed that increased level of CD and MDA in DEN control group was significantly declined by the nanoformulation of UFG.…”

Section: Discussionmentioning

confidence: 99%

Fabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies

Kumar¹,

Bhatt²,

Rahman³

et al. 2017

IJN

View full text Add to dashboard Cite

Umbelliferone β-D-galactopyranoside (UFG), isolated from plants, exhibits promising inhibitory action on numerous diseases. The present research was initiated to develop a suitable delivery system for UFG with an intention to enhance its therapeutic efficacy against diethyl nitrosamine (DEN)-induced hepatocellular carcinoma (HCC) in Wistar rats. UFG-loaded polymeric nanoparticles prepared by sonication were scrutinized for average size, drug loading capacity, zeta potential, and drug release potency in animals. HCC cell lines HuH-7 and Hep G2 were used for in vitro cytotoxic investigation. Several hepatic, nonhepatic, antioxidant, and anti-inflammatory biochemical parameters were estimated to establish the anticancer potential of UFG nanoformulation. Microscopical and histopathological investigations were also undertaken to substantiate the results of our work. Umbelliferone β-D-galactopyranoside-loaded poly(d,l-lactide- co -glycolide) nanoparticles (UFG-PLGA-NP) with particle size of 187.1 nm and polydispersity index 0.16 were uniform in nature with 82.5% release of the total amount of drug after 48 h. Our study successfully established the development and characterization of UFG-PLGA-NP with noticeable effect against both in vivo and in vitro models. The anticancer potential of UFG-PLGA-NP was brought about by the management of DEN-induced reactive oxygen species generation, mitochondrial dysfunction, proinflammatory cytokines alteration, and induction of apoptosis. Positive zeta potential on the surface of UFG-PLGA-NP would have possibly offered higher hepatic accumulation of UFG, particularly in the electron-dense mitochondria organelles, and this was the take-home message from this study. Our results demonstrated that such polymer-loaded delivery systems of UFG can be a better option and can be further explored to improve the clinical outcomes against hepatic cancer.

show abstract

“…Consequently reduction in oxidative stress and apoptosis. 106 Improving in insulin secretion increasing serum insulin and glutathione peroxidase activity 79 Anti-atherogenic Decrease in TAG and the TAG:HDL cholesterol (HDL-C) ratio, 52 Neuroprotective Neutralize ROS or enhance the expression of antioxidant gene, decrease in lactate/pyruvate ratio, extracellular nitric oxide, and lactase dehydrogenase generation Reduction in lipid oxidation and neuronal loss 107 108 Anti-menopausal symptoms Reduction in the number of hot flashes per day, Reduction in sum score of the Menopause Rating Scale II parameters in the treated group after 12 weeks but not significant in comparison to control group 109 …”

Section: Pharmacological Effect Mechanism(s) and Clinical Evidence(s)mentioning

confidence: 99%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

Protective role of Punica granatum (pomegranate) peel and seed oil extracts on diethylnitrosamine and phenobarbital-induced hepatic injury in male rats

Cited by 90 publications

References 34 publications

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Fabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies

Untitled

Contact Info

Product

Resources

About

Protective role of Punica granatum (pomegranate) peel and seed oil extracts on diethylnitrosamine and phenobarbital-induced hepatic injury in male rats

Cited by 90 publications

References 34 publications

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Fabrication, optimization, and characterization of umbelliferone &beta;-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies

Untitled

Contact Info

Product

Resources

About

Fabrication, optimization, and characterization of umbelliferone β-D-galactopyranoside-loaded PLGA nanoparticles in treatment of hepatocellular carcinoma: in vitro and in vivo studies