Protein A-conjugated iron oxide nanoparticles for separation ofVibrio choleraefrom water samples

Abstract

Pathogen separation is of great significance for precise detection and prevention of disease outbreaks. For the first time, protein A conjugated with chitosan-coated iron oxide nanoparticles was prepared for pathogen separation at low concentrations from liquid samples. Vibrio cholerae O1 (VO1) bacteria were used for testing the effectiveness of this conjugate. Transmission electron microscopy (TEM) was used to confirm the presence of captured VO1. The results showed that, after binding with a specific antibody, the conjugate allows separation of VO1 bacteria from water samples at a concentration as low as 10 cfu mL(-1). Moreover, the conjugate can be used in parallel with conventional or modern diagnostic tests for quick and accurate detection of pathogens.

Polyol synthesis, functionalisation, and biocompatibility studies of superparamagnetic iron oxide nanoparticles as potential MRI contrast agents

“…The nanoparticle surface provides the stability for these nanoparticles as well as their functionality by which biological moieties (antibodies, peptides, drug encapsulation) may potentially be grafted. [19][20][21][22][23][24] For instance, many studies have recently explored their therapeutic potential to kill cancer cells by hyperthermia (heat induced after the application of an alternating magnetic field) and simultaneous triggered drug delivery [25][26][27] . Furthermore, when a biological moiety (e.g., antibody, peptide) is conjugated to the surface of the nanoparticles, the latter may be actively targeted towards cancer cells to minimise any secondary effects from medical treatment may have on neighbouring healthy cells.…”

High performance multi-core iron oxide nanoparticles for magnetic hyperthermia: microwave synthesis, and the role of core-to-core interactions

“…Samples were prepared by pressing dried powders on a zero background silicon wafer. Field-dependent magnetisation, isothermal remanent magnetisation (IRM) and direct current demagnetisation (DCD) measurements of IONPs were carried out in a Quantum Design hybrid superconducting quantum interference device-vibrating sample magnetometer (SQUID-VSM) at 300 and 5 K, with applied fields up to 7 T. 57 Fe Mössbauer spectra were collected from freeze-dried samples mixed with boron nitride and measured in transmission mode in a commercial spectrometer (SEE Co Inc, USA) working in constant acceleration mode, calibrated relative to metallic α-Fe at room temperature (RT).…”

Functionalization strategies and dendronization of iron oxide nanoparticles

“…Small molecules like folic acid [113,114,115] or aptamers [116,117,118] and bigger molecules as peptides [119,120], proteins [121], or antibodies [122] can bind to many cells receptors and are used as targeting agents. …”