Protein adsorption on ZnO films studied by ATR-FTIR spectroscopy

Hinze, Wolfgang G.; Fallah, Maryam; Hauser, Karin

doi:10.3233/bsi-200199

Cited by 8 publications

(4 citation statements)

References 17 publications

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“…Thus, cell adhesion, spreading, proliferation, and other processes may be further induced by the configuration of the adsorbed protein layer. Therefore, protein adsorption on scaffolds plays a significant role in integrating a scaffold into the human body [38]. We used bovine serum albumin (BSA) as a model protein to investigate the protein adsorption capacity of PLA-TCP-ZnO scaffolds.…”

Section: Scaffold Structure Composition Mechanical Behavior and Surfa...mentioning

confidence: 99%

Additive manufacturing of bioactive and biodegradable poly (lactic acid)-tricalcium phosphate scaffolds modified with zinc oxide for guided bone tissue repair

Harb,

Kolanthai,

Pinto

et al. 2024

Biomed. Mater.

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Bioactive and biodegradable scaffolds that mimic the natural extracellular matrix of bone serve as temporary structures to guide new bone tissue growth. In this study, 3D-printed scaffolds composed of poly (lactic acid) (PLA)-tricalcium phosphate (TCP) (90-10 wt. %) were modified with 1%, 5%, and 10 wt. % of ZnO to enhance bone tissue regeneration. A commercial chain extender named Joncryl was incorporated alongside ZnO to ensure the printability of the composites. Filaments were manufactured using a twin-screw extruder and subsequently used to print 3D scaffolds via fused filament fabrication (FFF). The scaffolds exhibited a homogeneous distribution of ZnO and TCP particles, a reproducible structure with 300 µm pores, and mechanical properties suitable for bone tissue engineering, with an elastic modulus around 100 MPa. The addition of ZnO resulted in enhanced surface roughness on the scaffolds, particularly for ZnO microparticles, achieving values up to 241 nm. This rougher topography was responsible for enhancing protein adsorption on the scaffolds, with an increase of up to 85% compared to the PLA-TCP matrix. Biological analyses demonstrated that the presence of ZnO promotes mesenchymal stem cell (MSC) proliferation and differentiation into osteoblasts. Alkaline phosphatase (ALP) activity, an important indicator of early osteogenic differentiation, increased up to 29%. The PLA-TCP composite containing 5% ZnO microparticles exhibited an optimized degradation rate and enhanced bioactivity, indicating its promising potential for bone repair applications.

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Section: Scaffold Structure Composition Mechanical Behavior and Surfa...mentioning

confidence: 99%

Additive manufacturing of bioactive and biodegradable poly (lactic acid)-tricalcium phosphate scaffolds modified with zinc oxide for guided bone tissue repair

Harb,

Kolanthai,

Pinto

et al. 2024

Biomed. Mater.

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“…[36,37] Furthermore, there are some evidences, indicating a beneficial interaction between proteins and ZnO. [38][39][40][41] However, the protein-assisted in situ synthesis of ZnO in NR latex remains unreported.…”

Section: Introductionmentioning

confidence: 99%

Protein‐Mediated ZnO Synthesis for Self‐Healing in Natural Rubber

Fueangphakdee,

Nimpaiboon,

Junkong

2024

Macro Materials & Eng

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Natural rubber (NR) consists of mainly cis‐1,4‐polyisoprene with proteins as notable nonrubber components that significantly influence its properties. A relatively unexplored aspect is the role of these proteins in reducing and stabilizing metal ions to form metallic particles. Here, the NR/ZnO composite is successfully prepared by in situ generation of ZnO in the NR latex for the first time. With the aid of the proteins, the amount of ZnO is slightly higher than that formed in the saponified NR latex (SPNR), where some proteins are removed. Importantly, the noncovalent bonds between the proteins and/or amino acids with the ZnO formed in the NR latex can provide the self‐healing behavior of the NR/ZnO composite. However, heating at ≈40 °C is still necessary for reducing the healing time and increasing the self‐healing efficiency of the composite. Nevertheless, greater noncovalent bonds within NR/ZnO hinder rubber molecular chain interdiffusion and rearrangement essential for self‐healing behavior in uncross‐linked rubber, resulting in lower healing efficiency compared to SPNR/ZnO. This study underscores the significance of proteins and/or amino acids on NR's properties, despite their minor presence. Without sophisticated NR modification, this is a very simple and low‐cost technique to achieve the NR/ZnO composite with self‐healing behavior.

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“…This process is usually described at the equilibrium state based on the isotherms and kinetic and thermodynamic studies [ 3 , 4 , 5 ]. Additionally, characterization of the adsorbent, using, for example, FTIR, SEM, TEM, XRD, and XPS analysis before and after the formation of the adsorption layer, provides important information about the mechanisms of this phenomenon [ 6 , 7 , 8 ]. In many systems, the adsorption mechanism is complex and dependent on many factors relating to the solid, adsorbate, and solution properties [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Production of Activated Carbons from Food/Storage Waste

et al. 2023

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This paper deals with the adsorption of organic and inorganic pollutants on the surface of carbonaceous adsorbents prepared via the chemical activation of expired or broken food products—the solid residue of the “cola-type” drink as well as spoilt grains of white rice and buckwheat groats. The activation process was conducted in the microwave furnace with the use of two activating agents of different chemical nature—potassium carbonate and orthophosphoric acid. The activated carbons were characterized based on the results of elemental analysis, low-temperature nitrogen adsorption/desorption, Boehm titration, thermal analysis, and scanning electron microscopy. Additionally, the suitability of the materials prepared as the adsorbents of methylene blue and iodine from the aqueous solutions was estimated. The materials obtained via chemical activation with H3PO4 turned out to be much more effective in terms of both model pollutant adsorptions. The maximum sorption capacity toward iodine (1180 mg/g) was found for the white-rice-based activated carbon, whereas the most effective in the methylene blue removal (221.3 mg/g) was the sample obtained from the solid residue of the expired “cola-type” drink. For all carbonaceous materials, a better fit for the experimental adsorption data was obtained with the Langmuir isotherm model than the Freundlich one.

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Protein adsorption on ZnO films studied by ATR-FTIR spectroscopy

Cited by 8 publications

References 17 publications

Additive manufacturing of bioactive and biodegradable poly (lactic acid)-tricalcium phosphate scaffolds modified with zinc oxide for guided bone tissue repair

Additive manufacturing of bioactive and biodegradable poly (lactic acid)-tricalcium phosphate scaffolds modified with zinc oxide for guided bone tissue repair

Protein‐Mediated ZnO Synthesis for Self‐Healing in Natural Rubber

Production of Activated Carbons from Food/Storage Waste

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