Protein Adsorption to and Elution from Polyether Surfaces

Gombotz, Wayne R.; Wang, Guanghui; Horbett, Thomas A.; Hoffman, Allan S.

doi:10.1007/978-1-4899-0703-5_16

Cited by 45 publications

(24 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This two-step technique led to the first direct end-attachment of PEG molecules to a biomaterial surface, i.e., the first direct PEGylation of a surface. Our proposal was funded, and we found that, as the PEG MW increased, the amount of fibrinogen adsorption dropped sharply, and began to level out around PEG MWs of 1000-2500 [19][20][21]. These results were similar to those of Toray with radiation-grafted Poly(ethyleneglycolmethacrylate) or PEGMA.…”

Section: Statement Of Significancesupporting

confidence: 88%

“…Our mechanism suggested that the tight binding of water within a PEG chain that was end-linked to the surface would become important when the MW of the grafted PEG grew large enough for the polymer chain to fold over on itself and form a random coil that could bind and entrap water molecules within the coil. (e.g., [19][20][21] & Fig 3).…”

Section: Statement Of Significancementioning

confidence: 97%

See 1 more Smart Citation

The early days of PEG and PEGylation (1970s–1990s)

Hoffman

2016

Acta Biomaterialia

Self Cite

View full text Add to dashboard Cite

Section: Statement Of Significancesupporting

confidence: 88%

Section: Statement Of Significancementioning

confidence: 97%

The early days of PEG and PEGylation (1970s–1990s)

Hoffman

2016

Acta Biomaterialia

Self Cite

View full text Add to dashboard Cite

“…Kinsley and Nicell (2000) concluded that, it is unlikely that the hydroxyl bonding sites o f PEG are responsible for quenching the bonding sites on polymeric end products. Gombotz et al, (1992) demonstrated that PEG attached to a surface had the capability to reject proteins. Based on this fact, Buchanan and Nicell (1997) suggested that, PEG attached to the polymeric end products could be acting the same way to repel enzymes, which are essentially protein molecules.…”

Section: Use Of Additives To Suppress Inactivationmentioning

confidence: 99%

Laccase‐Catalyzed Removal of Diphenylamine from Synthetic Wastewater

Saha

Taylor

Bewtra

et al. 2008

Water Environment Research

View full text Add to dashboard Cite

The priority pollutant lists of both the U.S. Environmental Protection Agency (U.S. EPA) and the European Union (EU) include diphenylamine (DPA), a contaminant found in wastewater of various industries. This work demonstrates the potential of using enzymatic treatment to remove DPA from buffered synthetic wastewater. This treatment method includes oxidative polymerization of DPA using laccase from Trametes villosa, followed by removal of those polymers via adsorptive micellar flocculation (AMF) using sodium lauryl sulfate (SDS) and alum. Researchers investigated the effects of pH, laccase concentration, molecular mass, and concentration of polyethylene glycol (PEG) in continuously stirred batch reactors to achieve 95% substrate conversion in three hours. Treatment of 0.19 mM DPA was best at pH 7 and an enzyme concentration from 0.0025 to 0.0075 standard activity unit/mL. Except for PEG 400, optimum enzyme and PEG concentrations decreased with an increase in PEG molecular mass. Optimum AMF conditions were pH 3.0 to 6.5, 200 mg/L of SDS, and 150 mg/L of alum.

show abstract

“…The most well known of these are The interfacial behavior of proteins is of major concern PEG (poly(ethylene glycol) coatings (17,18), which are in many aspects of biomedical technology. For instance, it hydrophilic and uncharged with a high degree of conformais considered to be one of the dominating factors determining tional freedom.…”

Section: Introductionmentioning

confidence: 99%