Protein Adsorption to Surface Chemistry and Crystal Structure Modification of Titanium Surfaces

Jimbo, Ryo; Ivarsson, Mikael; Koskela, Anita; Sul, Young‐Taeg; Johansson, Carina B.

doi:10.5037/jomr.2010.1303

Cited by 47 publications

(39 citation statements)

References 43 publications

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“…Because osteocalcin is a strong indicator of osteoblast differentiation (Yonezawa et al, 2011), and temporal expression and maturation of extracellular matrix proteins such as fibronectin (Jimbo et al, 2007) or collagen type I (Franceschi and Iyer, 1992;Jimbo et al, 2010) are essential for mineralization, these results indicate that the rate of mineralization was enhanced for the test implants.…”

Section: Discussionmentioning

confidence: 71%

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

et al. 2011

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Nanostructured calcium phosphate (CaP) has been histologically and biomechanically proven to enhance osseointegration of implants; however, conventional techniques were not sufficiently sensitive to capture its biological effects fully. Here, we compared the conventional removal torque (RTQ) evaluation and gene expression in tissues around nanostructured CaP-coated implants, using real-time RT-PCR, with those of uncoated implants, in a rabbit model. At 2 wks, RTQ values were significantly higher, alkaline phosphatase (ALP) expression was significantly higher, and runt-related transcription factor 2 and tumor necrosis factor-α expressions were significantly lower in the coated than in the uncoated implants. This indicates that inflammatory responses were suppressed and osteoprogenitor activity increased around the CaP-coated surface. At 4 wks, although RTQ values did not significantly differ between the 2 groups, ALP and osteocalcin (OCN) were significantly up-regulated in the coated group, indicating progressive mineralization of the bone around the implant. Moreover, an osteoclast marker, adenosine triphosphatase, which indicates acidification of the resorption lacunae, was significantly higher for the coated implants, suggesting gradual resorption of the CaP coating. This study reveals detailed genetic responses to nanostructured CaP-coated implants and provides evidence that the effect of nanotopography is significant during the osseointegration cascade.

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Section: Discussionmentioning

confidence: 71%

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

et al. 2011

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“…However, its biological role has not been studied compared to other important bone related proteins [39]. Previous in vitro studies have identified the integrin subunit β1 as a mechanism involved in human osteoblast adhesion [27], and have suggested that laminin-1 recruits osteoprogenitor cells through an attachment effect [25,26].…”

Section: Discussionmentioning

confidence: 99%

In vitro Evaluation of Calcium Phosphate Precipitation on Possibly Bioactive Titanium Surfaces in the Presence of Laminin

Bougas

Stenport²,

Currie³

et al. 2011

JOMR

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ObjectivesThe aim of the present study was to evaluate calcium phosphate precipitation and the amount of precipitated protein on three potentially bioactive surfaces when adding laminin in simulated body fluid.Material and MethodsBlasted titanium discs were prepared by three different techniques claimed to provide bioactivity: alkali and heat treatment (AH), anodic oxidation (AO) or hydroxyapatite coating (HA). A blasted surface incubated in laminin-containing simulated body fuid served as a positive control (B) while a blasted surface incubated in non laminin-containing simulated body fuid served as a negative control (B-). The immersion time was 1 hour, 24 hours, 72 hours and 1 week. Surface topography was investigated by interferometry and morphology by Scanning Electron Microscopy (SEM). Analysis of the precipitated calcium and phosphorous was performed by Energy Dispersive X-ray Spectroscopy (EDX) and the adsorbed laminin was quantified by iodine (125I) labeling.ResultsSEM demonstrated that all specimens except for the negative control were totally covered with calcium phosphate (CaP) after 1 week. EDX revealed that B- demonstrated lower sum of Ca and P levels compared to the other groups after 1 week. Iodine labeling demonstrated that laminin precipitated in a similar manner on the possibly bioactive surfaces as on the positive control surface.ConclusionsOur results indicate that laminin precipitates equally on all tested titanium surfaces and may function as a nucleation center thus locally elevating the calcium concentration. Nevertheless further studies are required to clarify the role of laminin in the interaction of biomaterials with the host bone tissue.

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“…The energy state of the implant depends on the type of biomaterial, the handling during manufacturing, the mode of cleaning, sterilization, and needless to say, the handling of the implant during surgical procedure [13, 14]. In general, when the surface is positively charged, the surface turns hydrophilic and some of the plasma proteins essential for the initial osteogenic interactions adsorb to hydrophilic surfaces [15–17]. It has been suggested that the charge of the implant surface can be altered by oxidization [18], chemical and topographical modification [19, 20], and by plasma treatment [3, 14].…”

Section: Introductionmentioning

confidence: 99%

Plasma Treatment Maintains Surface Energy of the Implant Surface and Enhances Osseointegration

Guastaldi

Yoo

Marin

et al. 2013

International Journal of Biomaterials

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The surface energy of the implant surface has an impact on osseointegration. In this study, 2 surfaces: nonwashed resorbable blasting media (NWRBM; control) and Ar-based nonthermal plasma 30 days (Plasma 30 days; experimental), were investigated with a focus on the surface energy. The surface energy was characterized by the Owens-Wendt-Rabel-Kaelble method and the chemistry by X-ray photoelectron spectroscopy (XPS). Five adult beagle dogs received 8 implants (n = 2 per surface, per tibia). After 2 weeks, the animals were euthanized, and half of the implants (n = 20) were removal torqued and the other half were histologically processed (n = 20). The bone-to-implant contact (BIC) and bone area fraction occupancy (BAFO) were evaluated on the histologic sections. The XPS analysis showed peaks of C, Ca, O, and P for the control and experimental surfaces. While no significant difference was observed for BIC parameter (P > 0.75), a higher level for torque (P < 0.02) and BAFO parameter (P < 0.01) was observed for the experimental group. The surface elemental chemistry was modified by the plasma and lasted for 30 days after treatment resulting in improved biomechanical fixation and bone formation at 2 weeks compared to the control group.

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Protein Adsorption to Surface Chemistry and Crystal Structure Modification of Titanium Surfaces

Cited by 47 publications

References 43 publications

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

In vitro Evaluation of Calcium Phosphate Precipitation on Possibly Bioactive Titanium Surfaces in the Presence of Laminin

Plasma Treatment Maintains Surface Energy of the Implant Surface and Enhances Osseointegration

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