Protein and phytohormone profiles of Mahanarva spectabilis salivary glands infesting different forages

Abstract

Given the importance of pastures for feeding cattle, the study of factors that affect their productivity is essential to get plant material of higher nutritional quality. Thus, the study of insect–plant interaction is important for the development of control strategies. Pasture spittlebugs affect forage grasses causing severe damage. We tested hormone and protein profiles differentially expressed in the salivary glands of Mahanarva spectabilis when fed with different pasture genotypes. The LC/MS approaches combined with bioinformatics tools were used to identify the mains biological processes in the salivary glands. The grouping revealed a greater number of proteins involved in biological processes of metabolic synthesis, biotic/abiotic stress, and ion transport across the membrane. The proteomic profiles were altered when insects were fed with different grasses. We also detected phytohormones in the salivary glands involved in the modulation of defense responses in host plants. These results allowed the analysis of important biological processes such as cell homeostasis, stress proteins, nucleic acid metabolism, regulation of muscle contraction, and transport and export of biomolecules. This represents an important advance in the understanding of the plant–pest interaction and can contribute to the choice of target elicitors, which allow effective strategies in the control of pasture spittlebugs.

Análise de componentes moleculares da espuma e da toxina presente na glândula salivar de cigarrinha das pastagens

“…A presença de metabólitos e hormônios pode auxiliar na infestação, afetando o equilíbrio de vias de sinalização que desencadeiam respostas defensivas em gramíneas forrageiras. Monteiro et al (2021) detectou vários fitohormônios presentes na glândula salivar como etileno, ácido jasmônico, metil jasmonato, ácido abscisíco, ácido indolacético, zeatina e ácido salicílico, presentes na glândula salivar, envolvidos na modulação de respostas de defesas de plantas hospedeiras. Monteiro et al (2021) também identificou 36 proteínas na glândula salivar de M. Spectabilis presentes durante a alimentação de diferentes gramíneas forrageiras relacionadas a diversos processos biológicos como ribossomos, proteínas de exportação, fagossomas e proteínas de processos endoplasmáticos envolvidos com a síntese de proteínas; dentre outros processos regulatórios.…”