Protein arginine methyltransferases PRMT1, PRMT4/CARM1 and PRMT5 have distinct functions in control of osteoblast differentiation

Dashti, Parisa; Lewallen, Eric A.; Gordon, Jonathan A. R.; Montecino, Martín; Leeuwen, Johannes P. T. M. van; Stein, Gary S.; Eerden, Bram C. J. van der; Davie, James R.; Wijnen, André J. van

doi:10.1016/j.bonr.2023.101704

Cited by 5 publications

(2 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Uma inserção não codificante em GRHL2 causa superexpressão genética e múltiplas anomalias estruturais, incluindo fenda palatina, espinha bífida e encefalocele Research, Society and Development, v. 13, n. 2, e9713245010, 2024(CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v13i2.45010 (Crane-Smith et al, 2023. Ademais, existem reguladores epigenéticos que controlam o destino da linhagem osteogênica e a formação óssea que não são completamente elucidadas (Dashti et al, 2024).…”

Section: Resultsunclassified

Relato de caso de paciente com suposta Displasia Frontonasal: A complexidade de diagnosticar

Nardelli,

Laskoski,

Brum

et al. 2024

RSD

View full text Add to dashboard Cite

A displasia frontonasal representa um conjunto de malformações que afetam os olhos, o nariz e a região frontal da face. Neste estudo o objetivo foi relatar o caso clínico de um paciente do sexo masculino 46,XY, que apresentou características da displasia frontonasal, com malformações de face e crânio, bem como a dificuldade de diagnóstico correto. O paciente apresentou malformação congênita do tubo neural (encefalocele esfenoetmoidal), agenesia do corpo caloso, espasmo infantil, cicatriz coriorretiniana, anomalias craniofaciais incluindo: fissura pré-forame incompleta mediana, fissura rara de Tessier 0-14, colpocefalia, retrusão do meio da face, narina antevertida; atraso no desenvolvimento motor, redução da acuidade visual (estrabismo convergente no olho esquerdo), disfagia em fase oral, telangiectasias nas bochechas e na porção posterior da perna, autismo, fimose, bexiga neurogênica, pé varo corrigido com tala. A etiologia não foi definida, devido à complexidade diagnóstica e a acessibilidade a exames genéticos, mas o paciente apresenta uma condição bem definida dentro do espectro de displasias frontonasais: agenesia de corpo caloso, encefalocele basal, anomalias oculares e fissura da linha média. Neste caso, o aconselhamento genético leva à família ao conhecimento acessível sobre a condição de forma que possa contribuir no empoderamento e no acesso a tratamentos que melhorem a saúde integral do paciente.

show abstract

Section: Resultsunclassified

Relato de caso de paciente com suposta Displasia Frontonasal: A complexidade de diagnosticar

Nardelli,

Laskoski,

Brum

et al. 2024

RSD

View full text Add to dashboard Cite

show abstract

“…Although the seven variants are expressed differently between human tissues, isoforms 1–3 constitute the majority of PRMT1 in cells and are present in all tissues they tested. Two antibodies which recognize all splice variants at either the carboxy‐terminus epitope (Cell Signaling 2453) or the N‐terminus (Cell Signaling 2449) and which have previously been validated in a collection of cell lines (Dashti et al, 2023 ; Liu et al, 2023 ; Yao et al, 2021 ; Zhu et al, 2022 ) were used for PRMT1 detection. A standard curve created using recombinant PRMT1 allows for a correlation between band intensities and the amount of PRMT1 in the cell samples which, along with cell type specific estimates of cellular volume, was used to calculate cellular concentrations for the selected cell lines (Figure S1 ).…”

Section: Resultsmentioning

confidence: 99%

Oligomerization of protein arginine methyltransferase 1 and its effect on methyltransferase activity and substrate specificity

Rossi,

Nielson,

Ortolano

et al. 2024

Protein Science

View full text Add to dashboard Cite

Proper protein arginine methylation by protein arginine methyltransferase 1 (PRMT1) is critical for maintaining cellular health, while dysregulation is often associated with disease. How the activity of PRMT1 is regulated is therefore paramount, but is not clearly understood. Several studies have observed higher order oligomeric species of PRMT1, but it is unclear if these exist at physiological concentrations and there is confusion in the literature about how oligomerization affects activity. We therefore sought to determine which oligomeric species of PRMT1 are physiologically relevant, and quantitatively correlate activity with specific oligomer forms. Through quantitative western blotting, we determined that concentrations of PRMT1 available in a variety of human cell lines are in the sub‐micromolar to low micromolar range. Isothermal spectral shift binding data were modeled to a monomer/dimer/tetramer equilibrium with an EC50 for tetramer dissociation of ~20 nM. A combination of sedimentation velocity and Native polyacrylamide gel electrophoresis experiments directly confirmed that the major oligomeric species of PRMT1 at physiological concentrations would be dimers and tetramers. Surprisingly, the methyltransferase activity of a dimeric PRMT1 variant is similar to wild type, tetrameric PRMT1 with some purified substrates, but dimer and tetramer forms of PRMT1 show differences in catalytic efficiencies and substrate specificity for other substrates. Our results define an oligomerization paradigm for PRMT1, show that the biophysical characteristics of PRMT1 are poised to support a monomer/dimer/tetramer equilibrium in vivo, and suggest that the oligomeric state of PRMT1 could be used to regulate substrate specificity.

show abstract

Exploring epigenetic strategies for the treatment of osteoporosis

Yi,

Lim,

Kim

2024

Mol Biol Rep

View full text Add to dashboard Cite

Protein arginine methyltransferases PRMT1, PRMT4/CARM1 and PRMT5 have distinct functions in control of osteoblast differentiation

Cited by 5 publications

References 48 publications

Relato de caso de paciente com suposta Displasia Frontonasal: A complexidade de diagnosticar

Relato de caso de paciente com suposta Displasia Frontonasal: A complexidade de diagnosticar

Oligomerization of protein arginine methyltransferase 1 and its effect on methyltransferase activity and substrate specificity

Exploring epigenetic strategies for the treatment of osteoporosis

Contact Info

Product

Resources

About