Protein-based biomemory device consisting of the cysteine-modified azurin

Abstract

We demonstrated a protein-based memory device using recombinant Pseudomonas aeruginosa azurin (azurin), a metalloprotein with a redox property. Azurin was recombined with a cysteine residue to enhance the stability of the self-assembled protein on the gold surface. The memory device characteristics, including the “read,” “write,” and “erase” functions of the self-assembled azurin layer, were well demonstrated with three distinct electrical states of azurin layers by cyclic voltammetry. The robustness of the protein-based biomemory device was validated by the repeated electrochemical performance of 500000cycles.

Multilevel Biomemory Device Consisting of Recombinant Azurin/Cytochrome c

“…[10][11][12][13] Recently, the redox protein, blue copper azurin, was used in a biomemory device and the principles of this protein-based biomemory device were demonstrated in a previous study by our research group. [14,15] However, storing and reading out the multi-state of information, using proteins as the key element, with a simple fabrication method for the electronic device has not yet been reported.…”

Signal Enhancement of Electrochemical Biomemory Device Composed of Recombinant Azurin/Gold Nanoparticle

“…They developed and devised various electrochemicalbased biomemory devices using biomolecules on the bulk scale [15][16][17][18]. Biomemory function can be used to generate currents from biomolecules for the development of a molecular memory device.…”

Electrochemical biomemory device consisting of recombinant protein molecules

“…1. As a memory element, the redox property of protein is controlled by application of an external voltage to hold two electrically distinct positions [75,84,85]. If a reduction voltage is applied to protein, an electron flows into the protein core from the electrode and thereby entrapment of negative charge occurs in the protein layer, as depicted in Fig.…”

“…Even though programmable electrical bi-stability was realized in memory device that was constructed by organic polymer thin film and logical circuits have also been validated using bio and organic molecules as active components, these organic and biomolecules based electronic memory devices are currently in the early stage to validate the new concepts to develop organic/bio memory device as one of the alternatives [75][76][77].…”