Protein-Based Drug-Delivery Materials

Jao, Dave; Xue, Ye; Medina, Jethro; Hu, Xiao

doi:10.3390/ma10050517

Cited by 131 publications

(77 citation statements)

References 117 publications

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“…The use of keratin to produce functional biomaterials is widespread in different areas, ranging from applications in biomedicine [20,82] and drug-delivery [83], as natural polymer flocculants [84] and absorbents [85], in biolubricant formulations [86], to bioelectronics [87]. .…”

Section: Keratin-based Biomaterialsmentioning

confidence: 99%

Keratin Associations with Synthetic, Biosynthetic and Natural Polymers: An Extensive Review

Donato

Mija

2019

Polymers

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Among the biopolymers from animal sources, keratin is one the most abundant, with a major contribution from side stream products from cattle, ovine and poultry industry, offering many opportunities to produce cost-effective and sustainable advanced materials. Although many reviews have discussed the application of keratin in polymer-based biomaterials, little attention has been paid to its potential in association with other polymer matrices. Thus, herein, we present an extensive literature review summarizing keratin’s compatibility with other synthetic, biosynthetic and natural polymers, and its effect on the materials’ final properties in a myriad of applications. First, we revise the historical context of keratin use, describe its structure, chemical toolset and methods of extraction, overview and differentiate keratins obtained from different sources, highlight the main areas where keratin associations have been applied, and describe the possibilities offered by its chemical toolset. Finally, we contextualize keratin’s potential for addressing current issues in materials sciences, focusing on the effect of keratin when associated to other polymers’ matrices from biomedical to engineering applications, and beyond.

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Section: Keratin-based Biomaterialsmentioning

confidence: 99%

Keratin Associations with Synthetic, Biosynthetic and Natural Polymers: An Extensive Review

Donato

Mija

2019

Polymers

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“…[2][3][4][5] These materials were extensively investigated as drug delivery platforms. [6][7][8][9] Moreover, the blends of natural and synthetic polymers can be explored as a new class of materials that combine the mechanical properties and biocompatibility. 10,11 Silk may be one of the most promising candidates for an alternative delivery system.…”

Section: Introductionmentioning

confidence: 99%

Bioengineering the spider silk sequence to modify its affinity for drugs

Kucharczyk¹,

Weiss²,

Jastrzębska³

et al. 2018

IJN

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BackgroundSilk is a biocompatible and biodegradable material, able to self-assemble into different morphological structures. Silk structures may be used for many biomedical applications, including carriers for drug delivery. The authors designed a new bioengineered spider silk protein, EMS2, and examined its property as a carrier of chemotherapeutics.Materials and methodsTo obtain EMS protein, the MS2 silk monomer (that was based on the MaSp2 spidroin of Nephila clavipes) was modified by the addition of a glutamic acid residue. Both bioengineered silks were produced in an Escherichia coli expression system and purified by thermal method. The silk spheres were produced by mixing with potassium phosphate buffer. The physical properties of the particles were characterized using scanning electron microscopy, atomic force microscopy, Fourier-transform infrared spectroscopy, and zeta potential measurements. The MTT assay was used to examine the cytotoxicity of spheres. The loading and release profiles of drugs were studied spectrophotometrically.ResultsThe bioengineered silk variant, EMS2, was constructed, produced, and purified. The EMS2 silk retained the self-assembly property and formed spheres. The spheres made of EMS2 and MS2 silks were not cytotoxic and had a similar secondary structure content but differed in morphology and zeta potential values; EMS2 particles were more negatively charged than MS2 particles. Independently of the loading method (pre- or post-loading), the loading of drugs into EMS2 spheres was more efficient than the loading into MS2 spheres. The advantageous loading efficiency and release rate made EMS2 spheres a good choice to deliver neutral etoposide (ETP). Despite the high loading efficiency of positively charged mitoxantrone (MTX) into EMS2 particles, the fast release rate made EMS2 unsuitable for the delivery of this drug. A faster release rate from EMS2 particles compared to MS2 particles was observed for positively charged doxorubicin (DOX).ConclusionBy modifying its sequence, silk affinity for drugs can be controlled.

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“…Protein-based biopolymers are of great interest because they are water soluble, this makes them excellent candidates for use in the formulation of ODPs for hydrophobic chemotherapeutics [7]. ODPs using protein-based biopolymers are also essential in cancer therapy as they can enhance drug retention time, drug absorption and can be modified to impart targeted delivery [8]. Enhanced absorption and drug retention time lead to improve chemotherapeutic efficacy, while targeted delivery limits off-target cell toxicity.…”

Section: Protein-based Biopolymersmentioning

confidence: 99%

Nanotechnology-Based Biopolymeric Oral Delivery Platforms for Advanced Cancer Treatment

Chivere

Kondiah

Choonara

et al. 2020

Cancers

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Routes of drug administration and their corresponding physiochemical characteristics play major roles in drug therapeutic efficiency and biological effects. Each route of delivery has favourable aspects and limitations. The oral route of delivery is the most convenient, widely accepted and safe route. However, the oral route of chemotherapeutics to date have displayed high gastric degradation, low aqueous solubility, poor formulation stability and minimum intestinal absorption. Thus, mainstream anti-cancer drugs in current formulations are not suitable as oral chemotherapeutic formulations. The use of biopolymers such as chitosan, gelatin, hyaluronic acid and polyglutamic acid, for the synthesis of oral delivery platforms, have potential to help overcome problems associated with oral delivery of chemotherapeutics. Biopolymers have favourable stimuli-responsive properties, and thus can be used to improve oral bioavailability of anti-cancer drugs. These biopolymeric formulations can protect gastric-sensitive drugs from pH degradation, target specific binding sites for targeted absorption and consequently control drug release. In this review, the use of various biopolymers as oral drug delivery systems for chemotherapeutics will be discussed.

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Protein-Based Drug-Delivery Materials

Cited by 131 publications

References 117 publications

Keratin Associations with Synthetic, Biosynthetic and Natural Polymers: An Extensive Review

Keratin Associations with Synthetic, Biosynthetic and Natural Polymers: An Extensive Review

Bioengineering the spider silk sequence to modify its affinity for drugs

Nanotechnology-Based Biopolymeric Oral Delivery Platforms for Advanced Cancer Treatment

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