Protein Based Electrochemical Biosensors for H<sub>2</sub>O<sub>2</sub> Detection Towards Clinical Diagnostics

Yagati, Ajay Kumar; Choi, Jeong‐Woo

doi:10.1002/elan.201400037

Cited by 41 publications

(25 citation statements)

References 122 publications

(70 reference statements)

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“…1,2 Hence, development of highly sensitive and selective electrochemical biosensors using new strategies for sensor platforms is still in advancement. 3,4 Hydrogen peroxide (H 2 O 2 ) is one of the reactive oxygen species, which induce oxidative stress, growth arrest, apoptosis, or necrosis of cells 5 and also high concentration of H 2 O 2 leads to serious damage to human skin and health.…”

mentioning

confidence: 99%

Electrosynthesis of ERGO-NP Nanocomposite Films for Bioelectrocatalysis of Horseradish Peroxidase towards H₂O₂

Yagati

Min

Cho

2014

J. Electrochem. Soc.

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A simple and sensitive amperometric sensor was developed based on electrochemical co-reduction of graphene oxide/nanoparticle (ERGO-NP) composite films by chronoamperometry method on indium tin oxide (ITO) electrodes. By analyzing the properties of the composite films with experimental results and theoretical investigations, a comprehensive report on the physical and electrochemical interfacial properties of graphene mixed gold nanoparticles composite electrode is presented. The developed electrodes were applied for the detection of hydrogen peroxide (H 2 O 2 ) based on the direct electrochemistry of horseradish peroxidase (HRP). Standard carbodiimide chemistry enabled the covalent tethering of HRP on to the modified film thus maintaining its native structures, consequently facilitating the direct electron transfer between the protein and the underlying surface. The electrochemical result revealed that the ERGO-NP/ITO electrodes exhibited much higher conductivity (ca. 5 times) when compared with unmodified electrode. The developed sensor using amperometric measurements revealed high sensitivity (1808.9 μA.mM −1 cm −2 ), with detection limit (0.6 μM) achieved under optimized conditions toward H 2 O 2 . Further, the sensor showed good specificity, stability and reproducibility toward H 2 O 2 detection, therefore results proved that well-dispersed, high surface area and highly conductive modified surface could be a promising material for protein adsorption and fabrication of electrochemical biosensors.

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confidence: 99%

Electrosynthesis of ERGO-NP Nanocomposite Films for Bioelectrocatalysis of Horseradish Peroxidase towards H₂O₂

Yagati

Min

Cho

2014

J. Electrochem. Soc.

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“…A non-catalytic redox reaction and a bioelectrocatalytic H2O2-reduction of POD were reported. 66 However, most of the non-catalytic DET signals of POD were observed at around -0.2 V, which is assigned to the redox reaction of Fe 3+ /Fe 2+ of the protoheme that has no relation to the catalytic redox cycle. In addition, most of the catalytic waves of POD without a mediator reported in the literature have halfwave potentials approximately 0.2 V and the current densities are very low.…”

Section: Det-type Bioelectrocatalysis At Bod-and Pod-adsorbed Electrodesmentioning

confidence: 98%

Nanostructured Porous Electrodes by the Anodization of Gold for an Application as Scaffolds in Direct-electron-transfer-type Bioelectrocatalysis

et al. 2018

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In this study, nanostructured porous gold electrodes were prepared by the anodization of gold in the presence of oxalic acid or glucose as a reductant, and applied as scaffolds for direct electron transfer (DET)-type bioelectrocatalysis. Gold cations generated in the anodization seem to be reduced by the reductant to construct a porous gold structure. The DET-type performance of the electrode was examined using two DET-type model enzymes, bilirubin oxidase (BOD) and peroxidase (POD), for the four-electron reduction of dioxygen and the two-electron reduction of peroxide, respectively. BOD and POD on the anodized porous gold electrodes exhibited well-defined sigmoidal steady-state waves corresponding to DET-type bioelectrocatalysis. Scanning electron microscopy images revealed sponge-like pores on the electrodes. The anodized porous gold electrodes demonstrate promise as scaffolds for DET-type bioelectrocatalysis.

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“…Electrochemical sensing of H 2 O 2 has become crucial in industrial, clinical, food, and environmental settings, creating a constant need for simpler and more effective H 2 O 2 sensors, as well as methods for fabricating these sensors [1][2][3][4]. Also, H 2 O 2 is produced in many enzymatic reactions, which enables the detection of the chemical species involved in them [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Bimetallic PdCu/SPCE non-enzymatic hydrogen peroxide sensors

Uzunoğlu

Scherbarth

Stanciu

2015

Sensors and Actuators B: Chemical

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a b s t r a c tScreen printed carbon electrodes (SPCE) were decorated with PdCu bimetallic alloys via a facile coelectrodeposition method to develop disposable non-enzymatic H 2 O 2 sensors. The electrochemical performance of the biosensors was evaluated in terms of selectivity, sensitivity, and stability with a goal of demonstrating that employing a bimetallic PdCu non-enzymatic system can push forward the state of the art in hydrogen peroxide sensing. The physical characterization of the biosensors was conducted using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD) techniques. Sensors consisting of PdCu bimetals showed higher sensitivity than Pd/SPCE and Cu/SPCE electrodes toward H 2 O 2 . The fabricated PdCu/SPCE sensors showed a sensitivity of 396.7 A mM −1 cm −2 , a linear range from 0.5 mM to 11 mM, and a low limit of detection (0.7 M) at the applied potential of −0.3 V. The use of relatively low working potential eliminated the interference effect of the common electroactive species (ascorbic acid, uric acid, and glucose) present in a real sample, which are usually a concern for non-enzymatic sensing systems. In addition, the high reproducibility (RSD = 2.5%), and excellent long term stability render PdCu/SPCEs as attractive materials for the construction of disposable enzyme-free H 2 O 2 sensors.

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Protein Based Electrochemical Biosensors for H₂O₂ Detection Towards Clinical Diagnostics

Cited by 41 publications

References 122 publications

Electrosynthesis of ERGO-NP Nanocomposite Films for Bioelectrocatalysis of Horseradish Peroxidase towards H₂O₂

Electrosynthesis of ERGO-NP Nanocomposite Films for Bioelectrocatalysis of Horseradish Peroxidase towards H₂O₂

Nanostructured Porous Electrodes by the Anodization of Gold for an Application as Scaffolds in Direct-electron-transfer-type Bioelectrocatalysis

Bimetallic PdCu/SPCE non-enzymatic hydrogen peroxide sensors

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Protein Based Electrochemical Biosensors for H2O2 Detection Towards Clinical Diagnostics

Cited by 41 publications

References 122 publications

Electrosynthesis of ERGO-NP Nanocomposite Films for Bioelectrocatalysis of Horseradish Peroxidase towards H2O2

Electrosynthesis of ERGO-NP Nanocomposite Films for Bioelectrocatalysis of Horseradish Peroxidase towards H2O2

Nanostructured Porous Electrodes by the Anodization of Gold for an Application as Scaffolds in Direct-electron-transfer-type Bioelectrocatalysis

Bimetallic PdCu/SPCE non-enzymatic hydrogen peroxide sensors

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Protein Based Electrochemical Biosensors for H₂O₂ Detection Towards Clinical Diagnostics

Electrosynthesis of ERGO-NP Nanocomposite Films for Bioelectrocatalysis of Horseradish Peroxidase towards H₂O₂

Electrosynthesis of ERGO-NP Nanocomposite Films for Bioelectrocatalysis of Horseradish Peroxidase towards H₂O₂