Protein-Based Materials in Load-Bearing Tissue-Engineering Applications

Sayın, Esen; Baran, Erkan Türker; Hasırcı, Vasıf

doi:10.2217/rme.14.52

Cited by 25 publications

(14 citation statements)

References 102 publications

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“…22,30,31 Chitosan is a natural biopolymer composed of chitin, a long-chain polysaccharide of N-acetylglucosamine, displaying a structure very similar to that of GAGs. 32 The addition of collagen to the chitosan matrix allows for a composition more similar to that of the pre-dentin extracellular matrix, and provides the Arg-Gly-Asp (RGD) sequence required to promote cell migration and adhesion, 33 mimicking the natural basement membrane. 22 In the present study, a chitosan-collagen calcium-aluminate biomembrane was developed to simulate dentin composition.…”

Section: Discussionmentioning

confidence: 99%

Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells

et al. 2016

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The development of biomaterials capable of driving dental pulp stem cell differentiation into odontoblast-like cells able to secrete reparative dentin is the goal of current conservative dentistry. In the present investigation, a biomembrane (BM) composed of a chitosan/collagen matrix embedded with calcium-aluminate microparticles was tested. The BM was produced by mixing collagen gel with a chitosan solution (2:1), and then adding bioactive calcium-aluminate cement as the mineral phase. An inert material (polystyrene) was used as the negative control. Human dental pulp cells were seeded onto the surface of certain materials, and the cytocompatibility was evaluated by cell proliferation and cell morphology, assessed after 1, 7, 14 and 28 days in culture. The odontoblastic differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, total protein production, gene expression of DMP-1/DSPP and mineralized nodule deposition. The pulp cells were able to attach onto the BM surface and spread, displaying a faster proliferative rate at initial periods than that of the control cells. The BM also acted on the cells to induce more intense ALP activity, protein production at 14 days, and higher gene expression of DSPP and DMP-1 at 28 days, leading to the deposition of about five times more mineralized matrix than the cells in the control group. Therefore, the experimental biomembrane induced the differentiation of pulp cells into odontoblast-like cells featuring a highly secretory phenotype. This innovative bioactive material can drive other protocols for dental pulp exposure treatment by inducing the regeneration of dentin tissue mediated by resident cells.

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Section: Discussionmentioning

confidence: 99%

Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells

et al. 2016

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“…Among the increasing number of reports on implantable cell constructs, studies using natural biomaterials are rare. [ 141 ] To achieve end‐product development and clinical application or to meet the contradictory requirements for commercial approval, designer self‐assembling peptide hydrogels have good manufacturing practice (GMP) considerations to reconstruct bioengineering cell microenvironments in vitro for cell constructs formation, since their molecular motifs, mesh size, nanofiber porosity, biodegradability, flexible adaptive properties favor tissue‐specific cell growth for various cell types in body, in addition to various stem cells and cancer cells. [ 9,10 ] As illustrated in Figure 3, designer self‐assembling peptide hydrogels emulate the physiological relevance of native ECM in vivo and serve as the biomimetic hydrogels for a broad range of tissue‐specific cell constructs appropriate for in situ critical‐sized grafting or the injury site.…”

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Yang

Zhao

2020

Advanced Science

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mouse intestinal stem cells and primary colorectal cancer cells can be propagated in vitro long term, [1] there is an urgent need to develop more physiologically relevant, efficient, and robust precise oncology models that closely recapitulate the genetic and morphological heterogeneous composition and mimic the arrangement pattern of cancer cells in the original tumor. [2] To our knowledge in biomedical research, hydrogel is the best tool to reconstruct precise oncology models in vitro, especially tumor organoid, which not only recapitulates in vivo biology and microenvironmental factors, but also at large extent allows side-by-side comparison to evaluate the translational potential of 3D model systems to the patients. [2a,3] Generally, hydrogels are mainly composed of hydrophilic polymeric scaffolds that absorb large amounts of water, so the hydrogel matrix better mimics elastic and viscoelastic properties in ECM and microscale topographies of cell matrices, which controls proper cell morphology, directs viable cell behaviors, and drives in vivo fundamental cell-cell or cell-ECM interactions. [4] To recapitulate pathophysiological features of human tumors and imitate various aspects of human tumorigenesis in vivo, hydrogels can provide a realistic platform to establish a useful bridge between in vitro assays and in vivo cell microenvironments. In current biomedical applications, there are many kinds of hydrogels to be developed to mimic the biological properties of ECM, such Designer self-assembling peptides form the entangled nanofiber networks in hydrogels by ionic-complementary self-assembly. This type of hydrogel has realistic biological and physiochemical properties to serve as biomimetic extracellular matrix (ECM) for biomedical applications. The advantages and benefits are distinct from natural hydrogels and other synthetic or semisynthetic hydrogels. Designer peptides provide diverse alternatives of main building blocks to form various functional nanostructures. The entangled nanofiber networks permit essential compositional complexity and heterogeneity of engineering cell microenvironments in comparison with other hydrogels, which may reconstruct the tumor microenvironments (TMEs) in 3D cell cultures and tissue-specific modeling in vitro. Either ovarian cancer progression or recurrence and relapse are involved in the multifaceted TMEs in addition to mesothelial cells, fibroblasts, endothelial cells, pericytes, immune cells, adipocytes, and the ECM. Based on the progress in common hydrogel products, this work focuses on the diverse designer self-assembling peptide hydrogels for instructive cell constructs in tissue-specific modeling and the precise oncology remodeling for ovarian cancer, which are issued by several research aspects in a 3D context. The advantages and significance of designer peptide hydrogels are discussed, and some common approaches and coming challenges are also addressed in current complex tumor diseases.

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“…A unique group of natural proteins such as collagen, gelatin, silk fibroin, and fibrin has been studied in bone TE. Different forms of film, sponge, and fiber are generated using 3D scaffolds made partially, or totally of these proteins (Sayin et al, 2014). Collagen and denatured form of collagen, gelatin, possess excellent properties including low antigenicity, low inflammatory, and cytotoxic response and excellent cell compatibility (Ferreira et al, 2012).…”

Section: Bone Tementioning

confidence: 99%

State-of-Art Functional Biomaterials for Tissue Engineering

2019

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Nanobiotechnology-enabled tissue engineering strategies have emerged as an innovative and promising technique in the field of regenerative medical science. The design and development of multifunctional smart biomaterials compatible to human physiology is crucial to achieve the required biological function with a reduced negative biological response. Several medical bioimplants have been tested to boost life expectancy and better-quality life. The concept of biocompatibility focuses on body acceptance and no harmful effects after implantation, which require shaping the properties of materials synthesis, surface functionalization, and biofunctionality. Such developed bioactive and biodegradable materials have been utilized to achieve the required function at a specific period and sustainability to withstand the surrounding tissues for treating severe injuries and diseases. Thus, exploring new approaches to design multifunctional biocompatible advanced nanostructures to develop next-generation therapies for tissue engineering, this mini-review is an attempt to summarize the advancements in biofunctional smart materials. The review focuses on bio-mimic materials, biomaterials, self-assembly biomaterials, bioprinting functional hydrogels, new polymeric architectures, and hybrid synthetic-natural hydrogels in the field of tissue engineering and regenerative medicine (TERM). This mini-review will serve as a guideline to design future research where the selection of a smart multifunctional biomaterial is crucial to obtain best TERM performance.

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Protein-Based Materials in Load-Bearing Tissue-Engineering Applications

Cited by 25 publications

References 102 publications

Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells

Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

State-of-Art Functional Biomaterials for Tissue Engineering

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