Protein-based nanocarriers as promising drug and gene delivery systems

Elzoghby, Ahmed O.; Samy, Wael M.; Elgindy, Nazik A.

doi:10.1016/j.jconrel.2012.04.036

Cited by 728 publications

(434 citation statements)

References 130 publications

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“…Cell-laden cross-linking using a photoinitiator in conjunction with UV exposure can lead to cytotoxicity, while physical cross-linking results in lower mechanical stability compared to a covalently bonded hydrogel 4 . Nevertheless, because the cross-linking density, resulting mechanical properties and applicable residues are all well controlled by these methods, microfluidic mixers 7,8 , drug delivery colloids 9 and rare element mining 10 are promising applications, in addition to cell culturing for tissue engineering and regenerative medicine 4,8 . These techniques can produce large quantities of structures at reasonable speeds, although it is challenging to establish suitable microenvironments for biological studies.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Serien¹,

Sugioka²

2018

Opto-Electronic Advances

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Proteins are a class of biomaterials having a vast array of functions, including the catalysis of metabolic reactions, DNA replication, stimuli response and transportation of molecules. Recent progress in laser-based fabrication technologies has enabled the formation of three-dimensional (3D) proteinaceous micro-and nano-structures by femtosecond laser cross-linking, which has expanded the possible applications of proteins. This article reviews the current knowledge and recent advancements in the femtosecond laser cross-linking of proteins. An overview of previous studies related to fabrication using a variety of proteins and detailed discussions of the associated mechanisms are provided. In addition, advances and applications utilizing specific protein functions are introduced. This review thus provides a valuable summary of the 3D micro-and nano-fabrication of proteins for biological and medical applications.

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Section: Introductionmentioning

confidence: 99%

Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Serien¹,

Sugioka²

2018

Opto-Electronic Advances

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“…At concentrations above its critical micellization concentration (CMC, 0.5-2 mg/mL at pH∼7, 25°C), bCN self-assembles in water into nanometric micelles (∼20-25 nm in diameter) having a hydrophobic core and a hydrophilic corona (17). The bCN structural and physicochemical properties facilitate its application in drug delivery (18)(19)(20), and bCN has been used for encapsulation of hydrophobic chemotherapeutics (21)(22)(23), However, our formulation is quite different from these, and also from the Cx and casein-based formulation described recently (11). We assumed that in our formulation, bCN can allow high drug loading and encapsulation efficiency since the drug is encapsulated within bCN micelles present in significantly higher concentrations than its CMC.…”

Section: Introductionmentioning

confidence: 99%

Beta-casein nanocarriers of celecoxib for improved oral bioavailability

Perlstein¹,

Bavli²,

Turovsky

et al. 2014

European Journal of Nanomedicine

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Beta-casein (bCN) micelles were developed as a platform for improved oral bioavailability (BA) of poorly water-soluble drugs. Here we demonstrate a proof-ofconcept using the NSAID celecoxib (Cx) loaded into bCN micelles (Cx/bCN). In a crossover pharmacokinetic (PK) study in pigs (n = 4), dosed intraduodenally with either the commercial Cx formulation Celebra ® or Cx/bCN, the C max obtained after administration of Cx/bCN was 2.3-fold higher and the T max was 1.57-fold faster, leading to a 1.76-fold increase in the BA of Cx, compared to the Celebra ® formulation. It is suggested that this BA enhancement was caused by improvement of Cx solubility in intestinal fluids by bCN micelles, which maintained their Cx cargo in an amorphous state.

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“…1,2 The underlying rationale is their exceptional characteristics, namely biodegradability, nonantigenicity, abundant renewable sources, extraordinary binding capacity with various drugs, and the ease of scaling up during manufacture.…”

Section: Introductionmentioning

confidence: 99%

Novel ionically crosslinked casein nanoparticles for flutamide delivery: formulation, characterization, and in vivo pharmacokinetics

Elzoghby¹,

Helmy²,

Samy³

et al. 2013

IJN

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A novel particulate delivery matrix based on ionically crosslinked casein (CAS) nanoparticles was developed for controlled release of the poorly soluble anticancer drug flutamide (FLT). Nanoparticles were fabricated via oil-in-water emulsification then stabilized by ionic crosslinking of the positively charged CAS molecules below their isoelectric point, with the polyanionic crosslinker sodium tripolyphosphate. With the optimal preparation conditions, the drug loading and incorporation efficiency achieved were 8.73% and 64.55%, respectively. The nanoparticles exhibited a spherical shape with a size below 100 nm and a positive zeta potential (+7.54 to +17.3 mV). FLT was molecularly dispersed inside the nanoparticle protein matrix, as revealed by thermal analysis. The biodegradability of CAS nanoparticles in trypsin solution could be easily modulated by varying the sodium tripolyphosphate crosslinking density. A sustained release of FLT from CAS nanoparticles for up to 4 days was observed, depending on the crosslinking density. After intravenous administration of FLT-CAS nanoparticles into rats, CAS nanoparticles exhibited a longer circulation time and a markedly delayed blood clearance of FLT, with the half-life of FLT extended from 0.88 hours to 14.64 hours, compared with drug cosolvent. The results offer a promising method for tailoring biodegradable, drug-loaded CAS nanoparticles as controlled, long-circulating drug delivery systems of hydrophobic anticancer drugs in aqueous vehicles.

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Protein-based nanocarriers as promising drug and gene delivery systems

Cited by 728 publications

References 130 publications

Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Beta-casein nanocarriers of celecoxib for improved oral bioavailability

Novel ionically crosslinked casein nanoparticles for flutamide delivery: formulation, characterization, and in vivo pharmacokinetics

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