Protein composition of the cell envelope of the bacterium Shewanella frigidimarina Pi 2-35 (Gammaproteobacteria: Shewanellaceae)

Khomenko, V. A.; Likhatskaya, G. N.; Romanenko, Lyudmila A.; Portnyagina, O. Yu.; Solovyeva, T. F.; Novikova, O. D.

doi:10.1134/s1063074016010107

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…Purified porin reconstituted in BLM at a concentration 10–100 ng/mL induced stepwise changes in membrane conductance typical for porins. It is noteworthy that the activity of marine porins [ 17 , 18 ] as a rule, is lower (at 100–200 ng/mL) as compared to the activity of Enterobacteriaceae porins (at 1–10 ng/mL) [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…The present study is a continuation of a series of our investigations that are dedicated to the isolation and characterization of functional activities of porins from the OM of marine microorganisms [ 17 , 18 ]. In this study, namely, we have isolated and characterized functional activity and spatial structure of porin from OM of Marinomonas primoryensis KMM 3633 T .…”

Section: Introductionmentioning

confidence: 99%

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Porin from Marine Bacterium Marinomonas primoryensis KMM 3633T: Isolation, Physico-Chemical Properties, and Functional Activity

et al. 2020

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Marinomonas primoryensis KMM 3633T, extreme living marine bacterium was isolated from a sample of coastal sea ice in the Amursky Bay near Vladivostok, Russia. The goal of our investigation is to study outer membrane channels determining cell permeability. Porin from M. primoryensis KMM 3633T (MpOmp) has been isolated and characterized. Amino acid analysis and whole genome sequencing were the sources of amino acid data of porin, identified as Porin_4 according to the conservative domain searching. The amino acid composition of MpOmp distinguished by high content of acidic amino acids and low content of sulfur-containing amino acids, but there are no tryptophan residues in its molecule. The native MpOmp existed as a trimer. The reconstitution of MpOmp into black lipid membranes demonstrated its ability to form ion channels whose conductivity depends on the electrolyte concentration. The spatial structure of MpOmp had features typical for the classical gram-negative porins. However, the oligomeric structure of isolated MpOmp was distinguished by very low stability: heat-modified monomer was already observed at 30 °C. The data obtained suggest the stabilizing role of lipids in the natural membrane of marine bacteria in the formation of the oligomeric structure of porin.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Porin from Marine Bacterium Marinomonas primoryensis KMM 3633T: Isolation, Physico-Chemical Properties, and Functional Activity

et al. 2020

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Adaptation Potential of Three Psychrotolerant Aquatic Bacteria in the Pan-Okhotsk Region

Voronina

Kunda

Рыжова

et al. 2022

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The Pan-Okhotsk region, which is part of the western North Pacific Ocean, is famous for its active volcanoes, which are part of the Pacific Ring of Fire and that enrich the surrounding waters with essential chemicals. Therefore, this region, including the Sea of Okhotsk and the Sea of Japan, is characterized by rich biota. Bacterioplankton plays a significant part in biological communities and is an indicator of ecosystem function. Analyzing the adaptability of three representatives of the microbiota of the Pan-Okhotsk region was the goal of our investigation. Marinomonas primoryensis KMM3633T (MP), Yersinia ruckeri KMM821 (YR), and Yersinia pseudotuberculosis 598 (YP) from the G.B. Elyakov Pacific Institute of Bioorganic Chemistry were studied by means of genomic and bioinformatic methods. The list of membrane translocator proteins, metabolism pathways, and cold shock and antifreeze proteins that were revealed in the genome of MP characterized this bacterium as being adaptable to free living in marine conditions, even at winter temperatures. The genomic potential of YR and YP makes not only survival in the environment of the Pan-Okhotsk region but also pathogenesis in eukaryotic organisms possible. The data obtained will serve as a basis for further ecosystem monitoring with the help of microbiota research.

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Protein composition of the cell envelope of the bacterium Shewanella frigidimarina Pi 2-35 (Gammaproteobacteria: Shewanellaceae)

Cited by 2 publications

References 19 publications

Porin from Marine Bacterium Marinomonas primoryensis KMM 3633T: Isolation, Physico-Chemical Properties, and Functional Activity

Porin from Marine Bacterium Marinomonas primoryensis KMM 3633T: Isolation, Physico-Chemical Properties, and Functional Activity

Adaptation Potential of Three Psychrotolerant Aquatic Bacteria in the Pan-Okhotsk Region

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