Protein corona-coated immunomagnetic nanoparticles with enhanced isolation of circulating tumor cells

Jiang, Xinbang; Zhang, Xiangyun; Guo, Chen; Yu, Yameng; Ma, Boya; Liu, Zhuang; Chai, Yamin; Wang, Lichun; Du, Yunzheng; Wang, Biao; Li, Nan; Dong, Dong; Li, Yueguo; Ou, Lailiang

doi:10.1039/d2nr01568d

Cited by 13 publications

(7 citation statements)

References 40 publications

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“…As shown in Figure f, the capture performance of IMNs was 70.5%, which was lower than that in PBS (77.8%). This may be because the antibody recognition site was shielded by the protein corona formation on particles when encountered with blood proteins. , However, the capture ability of IP-CMNs (88.3%) in artificial blood samples was higher than in PBS (83.7%). These results illustrated that the IP-CMNs toward CTCs were more sensitive than IMNs.…”

Section: Resultsmentioning

confidence: 98%

“…Recent studies have reported that a preformed protein corona, serving as a protective coating for nanoparticles, could reduce the nonspecific protein binding upon blood administration and increase the escape ability toward macrophages to prolong the circulation time. − Furthermore, some reports demonstrated that protein pre-adsorption may be required as a stealth layer of nanoparticles to prevent nonspecific cellular uptake. , A high cellular internalization of nanoparticles was observed when the cells were cultured without serum proteins. In contrast, no significant cellular uptake of the stealth nanoparticles precoated with proteins was found.…”

Section: Introductionmentioning

confidence: 99%

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Dual Stealth Functional Immuno-magnetic Nanoparticles for High-Performance Isolation of Circulating Tumor Cells

Jiang

Sun

et al. 2023

Anal. Chem.

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As a biomarker of hepatocellular carcinoma (HCC) biopsy, circulating tumor cells (CTCs) are often used in the diagnosis of cancer and treatment guidance. For CTCs detection, immuno-magnetic nanoparticles (IMNs) are one of the most commonly used platforms. However, the nonspecific adsorption of proteins and non-tumor cells weakens the performance of IMNs to capture CTCs. In this work, we developed an IMNs platform which was constructed by a biomimetic protein corona precoating and a polyethylene glycol (PEG) spacer to form the PEG and corona-coated IMNs (IP-CMNs). Due to the dual stealth effect of protein corona precoating and PEG spacer, the nonspecific protein adsorption and cell binding of P-CMNs could reduce by ∼5.5- and ∼5.4-fold, respectively, compared with those of unmodified particles. Furthermore, the PEG spacer could not only reduce the interaction between IP-CMNs and leukocytes but also enhance the capture performance toward tumor cells. By using artificial blood samples, the capture efficiency of IP-CMNs toward rare CTCs was found to be 88.3%, while it was 70.5% by using commercial IMNs. Finally, CTCs were successfully isolated in all HCC patient blood samples (7/7) using IP-CMNs. These results provide insight into the use of the multifunctional nanoplatform as a useful tool for CTCs detection.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Dual Stealth Functional Immuno-magnetic Nanoparticles for High-Performance Isolation of Circulating Tumor Cells

Jiang

Sun

et al. 2023

Anal. Chem.

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“…Blood purification materials such as activated carbon, polysaccharides, − and synthetic resins , are widely used because of their large specific surface area, excellent biocompatibility, ease of synthesis, and large exchange capacity. The development of new adsorbent materials with various continues apace, such as new carbon materials, magnetic nanoparticles, natural and synthetic polymers, composite materials, , and renewable stimuli-responsive adsorbents , has been applied to the removal of harmful substances from blood. These new materials exhibit greatly improved adsorption performance and excellent biocompatibilityan important criterion for evaluating hemoperfusion adsorbents.…”

Section: Introductionmentioning

confidence: 99%

An Evolutionary Review of Hemoperfusion Adsorbents: Materials, Preparation, Functionalization, and Outlook

Guo,

Jiang,

Guo

et al. 2024

ACS Biomater. Sci. Eng.

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Accumulation of pathogenic factors in the blood may cause irreversible damage and may even be life-threatening. Hemoperfusion is an effective technique for eliminating pathogenic factors, which is widely used in the treatment of various diseases including liver failure, renal failure, sepsis, and others. Hemoperfusion adsorbents are crucial in this process as they specifically bind and remove the target pathogenic factors. This review describes the development of hemoperfusion adsorbents, detailing the different properties exhibited by inorganic materials, organic polymers, and new materials. Advances in natural and synthetic polymers and novel materials manufacturing techniques have driven the expansion of hemoperfusion adsorbents in clinical applications. Stimuli-responsive (smart responsive) adsorbents with controllable molecular binding properties have many promising and environmentally friendly biomedical applications. Knowledge gaps, future research directions, and prospects for hemoperfusion adsorbents are discussed.

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“…[1,2] Therefore, isolation and enumeration of CTCs DOI: 10.1002/adfm.202304426 may play an important role in early cancer diagnosis and treatment progress monitoring. [3,4] In recent years, numerous platforms for the detection of CTCs have been developed to isolate CTCs from whole blood, such as immunomagnetic nanoparticles (IMNs), [5][6][7] antibody-conjugated microfluidic chips, [8][9][10] and size exclusion filtration. [11][12][13] Among them, IMNs have attracted extensive attention owing to their easy manipulation, high capture performance, and rapid isolation.…”

Section: Introductionmentioning

confidence: 99%

Genetically Engineered Cell Membrane‐Coated Magnetic Nanoparticles for High‐Performance Isolation of Circulating Tumor Cells

Jiang,

Zhang,

Guo

et al. 2023

Adv Funct Materials

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Circulating tumor cells (CTCs) are a crucial biomarker for early cancer diagnosis and progress of cancer metastasis. However, the extremely rare CTCs with large amounts of background leukocytes seriously restricte the purity of enriched CTCs. Herein, genetically engineered cell membrane‐coated magnetic nanoparticles with following properties are constructed: I) The leukocyte membrane camouflaged nanoparticles, which could significantly reduce nonspecific binding of homologous leukocytes and achieve high‐purity isolation of CTCs, are prepared by simple one‐step extrusion and II) the cellular membrane stably expressing single‐chain variable fragment (scFv) of anti‐epidermal growth factor receptor (EGFR) antibody could ensure the recognition of EGFR‐positive CTCs. The binding affinity of the resulting nanoparticles toward extracellular EGFR protein improved more than 100‐fold compared with natural cell membrane‐coated nanoparticles. Furthermore, compared with commercial immunomagnetic nanoparticles, the functional nanoparticles achieved a greater capture efficiency in artificial blood samples and the cell purity increased from 64.8% to 93.5%. Consequently, the authors successfully isolate high pure CTCs from 6 out of 6 cancer patients using engineered cell membrane‐coated nanoparticles. This platform exhibits a promising potential for CTC detection in clinical samples, offering an innovative method for cancer diagnosis and prognosis evaluation.

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Protein corona-coated immunomagnetic nanoparticles with enhanced isolation of circulating tumor cells

Abstract: Immunomagnetic nanoparticles (IMNs) have been widely developed as a detection tool to isolate rare circulating tumor cells (CTCs) from whole blood as a potential method for early cancer diagnosis, metastasis...

Cited by 13 publications

References 40 publications

Dual Stealth Functional Immuno-magnetic Nanoparticles for High-Performance Isolation of Circulating Tumor Cells

Dual Stealth Functional Immuno-magnetic Nanoparticles for High-Performance Isolation of Circulating Tumor Cells

An Evolutionary Review of Hemoperfusion Adsorbents: Materials, Preparation, Functionalization, and Outlook

Genetically Engineered Cell Membrane‐Coated Magnetic Nanoparticles for High‐Performance Isolation of Circulating Tumor Cells

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