Protein detection with a novel ISFET-based zeta potential analyzer

Recent advances in biologically sensitive field-effect transistors (BioFETs)

“…The required measurement principles for this can rely on coulometric titrant generation, pH-step titration and ion-step meth- ods. 151,152 As an example, the lysozyme concentration was measured by means of the pH-step method in the concentration range from 3 3 10 27 to 3 3 10 24 M. 151 Another approach is proposed 153,154 where the authors have demonstrated the feasibility of an ISFET-based immunosensor using the streaming potential (or zeta potential) measurement set-up.…”

ISFET pH sensor characterization: towards biosensor microchip application

“…Because of the advantages, the ISFET can be widely used in many areas especially in biomedical areas such as medical diagnostics, monitoring clinical or environmental samples, fermentation and bioprocess control and testing pharmaceutical or food products [2]. An ISFET-based Penicillin sensor [3], ISFET-based zeta potential analyzer (protein detection) [4], urea detection [5] and ISFET glucose sensor [6] are the several examples of ISFET application in medical area. As a milestone, the ISFET pH sensor has been designed and simulated using Tanner Tools (L-Edit, S-Edit and Tspice), in order to develop the biosensor microchip.…”

Impedimetric or Ion‐Sensitive Field‐Effect Transistor (ISFET) Aptasensors Based on the Self‐Assembly of Au Nanoparticle‐Functionalized Supramolecular Aptamer Nanostructures

“…Ion-sensitive field-effect transistor (ISFET) devices find growing interest as electronic transducers that follow biorecognition or biocatalytic transformations [25 -27]. The changes in the dielectric properties of the gate of the FET device as a result of the formation of antigenantibody [28,29] or protein-protein complexes [30], and the changes in the charge of the gate surface as a result of biocatalyzed transformations [31,32] or DNA hybridization [33 -35] were used to develop FET-based sensor devices. Also, the label-free detection of aptamer-substrate complexes was demonstrated by the dissociation of a duplex DNA through the formation of the aptamer-substrate complex on the gate surface of the FET device [6].…”