Protein digestion in cereal aphids (Sitobion avenae) as a target for plant defence by endogenous proteinase inhibitors

Pyati, Prashant; Bandani, Ali Reza; Fitches, Elaine; Gatehouse, John A.

doi:10.1016/j.jinsphys.2011.03.024

Cited by 48 publications

(62 citation statements)

References 32 publications

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“…diSCuSSion This is the first study bringing evidence that aphid species do possess carbohydrases, especially α-amylase enzyme for digestion of nutrient ingesting during feeding. It has already been reported that aphids use proteinases (endoprotease) in order to digest peptides and proteins ingested by the insect (Cristofoletti et al 2003;Pyati et al 2011). The phloem sap of the plants on which the aphids feed reportedly contains various molecules including proteins, small peptides, some enzymes, digestive enzyme inhibitors, and lec- tins (Kehr 2006).…”

Section: Methodsmentioning

confidence: 99%

“…These molecules could be used by the aphids as potential nitrogen source. So, the old view that the aphids do not carry out hydrolysis of the ingested material inside the gut is no more valid (Pyati et al 2011). Major protease that the aphids use to digest proteins in the gut seems to be derived from both groups of cysteine and serine proteinases (Cristofoletti et al 2003;Rahbe et al 2003;Pyati et al 2011), although cysteine proteinases are likely to be more widespread (Terra & Ferreira 2005).…”

Section: Methodsmentioning

confidence: 99%

“…Native-PAGE gel electrophoresis of α-amylase of two aphid species, A. fabae (A) and A. gossypii (B) presence of a number of isoenzymes in the alimentary canal of the insect is a strategy of how to suppress the inhibitory activity of the digestive enzyme inhibitor/s present in the diet in nature (Silva et al 1999). It is interesting to note that optimum pH for the α-amylase activity was neutral (7.0) which is corresponding to optimum pH of proteinase in the aphid gut (Pyati et al 2011).…”

Section: Aphis Faba Aphis Gossypiimentioning

confidence: 99%

“…There is an old view that aphids as plant sap feeders are not capable of digesting the ingested materials. This view has been questioned because of the existence of both biochemical and molecular evidence suggesting that this insect also uses hydrolytic enzyme to digest the ingested materials (Foissac et al 2002;Pyati et al 2011). In addition to the free amino acids, phloem sap contains polymers such as lectins, proteins, and peptides that should be digested in the insect's alimentary canal before absorption (Kehr 2006).…”

mentioning

confidence: 99%

“…Cysteine proteinase (Cathepsin L) is the major protease in the gut of A. gossypii, Acyrthosiphon pisum, and sitobion avenae (Cristofoletti et al 2003;Deraison et al 2004;Pyati et al 2011). In addition to Cathepsin L, Cathepsin B is reported to be present in the aphid gut (Rispe et al 2007).…”

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confidence: 99%

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Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)

Darvishzadeh¹,

Bandani²,

Mousavi³

2014

Plant Prot. Sci.

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Darvishzadeh A., Bandani A.R., Mousavi S.Q. (2014): Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae). Plant Protect. Sci., 50: 84-89.We identify and characterise α-amylases of the two aphid species, A. fabae and A. gossypi. To do this, α-amylases of the two insect species were extracted and their activities were determined using 1% soluble starch. Results showed that α-amylase, which hydrolyses starch, is present in both aphids. Also, it was shown that optimum pH and temperature for the α-amylases of both species is 7.0 and 40°C, respectively. Gel assays using zymogram analysis showed that in both aphid species more than one isoform (two isoforms) of α-amylases hydrolyszing carbohydrates are present.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Aphis Faba Aphis Gossypiimentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)

Darvishzadeh¹,

Bandani²,

Mousavi³

2014

Plant Prot. Sci.

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Breaching the Sieve Element—The Role of Saliva as the Molecular Interface Between Aphids and the Phloem

Will

Carolan

Wilkinson

2012

Phloem

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Aphids (order Hemiptera; suborder Sternorrhyncha) are fast becoming an excellent system for studying molecular interactions between sap feeding insects and their host plants. This has been facilitated, in part, by the recent completion of the pea aphid (Acyrthosiphon pisum) genome project (IAGC, 2010) and the development of high throughput genomic resources such as preliminary genomes and large-scale EST libraries for a number of other aphid species. Coupled with a strong tradition as a model system for investigating insect-plant interactions more generally, the recent increase in the number of studies involving aphids has lead to greater insights into the complexity of plant-aphid interactions and has provided a tantalizing preview of the intricate molecular mechanisms that govern this intimate association. Aphids and other sap-feeding hemipterans (e.g., whiteflies) have evolved specialized mouthparts, the stylets, which penetrate through plant tissues to feed directly from a single sieve element within the phloem. The feeding pathway, mechanism of penetration, and insect behavior associated with feeding have been well described (reviewed by Powell et al., 2006), but the role of the copious amounts of saliva that is continuously secreted during feeding remains obscure. Two types of saliva are recognized: a "gelling" saliva that constitutes the salivary sheath that is left in situ following stylet withdrawal from plant tissues, and a "watery" saliva that is secreted intracellularly either when the stylets briefly puncture cells during probing or immediately before and during sap ingestion (Martin et al., 1997). The gelling saliva is thought to provide mechanical support to the delicate stylets and to offer a degree of molecular concealment from plant defenses (Miles, 1999; Tjallingii, 2006; Will et al., 2007), although the assignment of function to the various types of saliva remains speculative, largely because of difficulties with collection and analysis of aphid saliva in general.

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A new gene from Xenorhabdus bovienii and its encoded protease inhibitor protein against Acyrthosiphon pisum

Zeng

Xue

Zhang

et al. 2012

Pest Management Science

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Protein digestion in cereal aphids (Sitobion avenae) as a target for plant defence by endogenous proteinase inhibitors

Cited by 48 publications

References 32 publications

Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)

Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)

Breaching the Sieve Element—The Role of Saliva as the Molecular Interface Between Aphids and the Phloem

A new gene from Xenorhabdus bovienii and its encoded protease inhibitor protein against Acyrthosiphon pisum

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