Protein-Excipient Interactions Evaluated via Nuclear Magnetic Resonance Studies in Polysorbate-Based Multidose Protein Formulations: Influence on Antimicrobial Efficacy and Potential Study Approach

Torosantucci, Riccardo; Furtmann, Britta; Elshorst, Bettina; Pfeiffer-Marek, Stefania; Hartleb, Tanja; Andres, Nikolaus; Bussemer, Till

doi:10.1016/j.xphs.2018.05.021

Cited by 14 publications

(13 citation statements)

References 8 publications

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“…Certainly, it may depend on the colloidal and surface-active properties of the protein (Koepf et al 2018a, b). Tweens, for example, can interact directly with the protein, and especially with exposed hydrophobic protein surfaces, which leads to an improvement of protein solubility and stability, or they may interact with other excipients present in the formulation (Torosantucci et al 2018). On the other hand, Tweens may compete with proteins for adsorption at the air-water interface, thus hindering proteins from adsorbing to hydrophobic interfaces.…”

Section: Introductionmentioning

confidence: 99%

Albumin displacement at the air–water interface by Tween (Polysorbate) surfactants

et al. 2020

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Tween (polysorbate) 20 and 80 are surfactants used for the development of parenteral protein drugs, due to their beneficial safety profile and stabilisation properties. To elucidate the mechanism by which Tween 20 and 80 stabilise proteins in aqueous solutions, either by a “direct” protein to surfactant interaction and/or by an interaction with the protein film at the air–water interface, we used spectroscopic (Infrared Reflection Absorption Spectroscopy, IRRAS) and microscopic techniques (Brewster Angle Microscopy, BAM) in combination with surface pressure measurements. To this end, the impact of both types of Tweens with regard to the displacement of the protein from the air–water interface was studied. As a model protein, human serum albumin (HSA) was used. The results for the displacement of the adsorbed HSA films by Tweens 20 and 80 can partially be understood on the basis of an orogenic displacement mechanism, which depends on the critical surface pressure of the adsorbed protein film. With increasing concentration of Tween in the sub-phase, BAM images showed the formation of different domain morphologies. IRRA-spectra supported the finding that at high protein concentration in the sub-phase, the protein film could not be completely displaced by the surfactants. Comparing the impact of both surfactants, we found that Tween 20 adsorbed faster to the protein film than Tween 80. The adsorption kinetics of both Tweens and the speed of protein displacement increased with rising surfactant concentration. Tween 80 reached significant lower surface pressures than Tween 20, which led to an incomplete displacement of the observed HSA film.

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Section: Introductionmentioning

confidence: 99%

Albumin displacement at the air–water interface by Tween (Polysorbate) surfactants

et al. 2020

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“…28 Similarly, Torosantucci et al showed that the antimicrobial effectiveness of a PH-containing fusion protein was reduced due to the binding of PH to the fusion protein. 32 We, therefore, down-selected 8 two-AP combinations that displayed higher log reduction to meet the 24-hour B criteria (≥ 2 log reduction). The AH-containing placebo formulation was also used for AET testing during the statistically-designed three-AP DOE screening experiments.…”

Section: Lessons Learned For Aet Testing During Ap Combination Screen...mentioning

confidence: 99%

Multi-Dose Formulation Development for a Quadrivalent Human Papillomavirus Virus-Like Particle-Based Vaccine: Part I - Screening of Preservative Combinations

Jerajani

Wan²,

Kumru³

et al. 2023

Journal of Pharmaceutical Sciences

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“…Therefore, PS-based drug carriers can carry multiple drugs for combination therapy. Particularly, PS-drug conjugates and PS-coated drug carriers have great potential to treat central nervous system-related diseases because PS coatings can improve the crossing of the BBB [ 77 ].…”

Section: Coating Polymersmentioning

confidence: 99%

Management of Brain Cancer and Neurodegenerative Disorders with Polymer-Based Nanoparticles as a Biocompatible Platform

et al. 2023

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The blood–brain barrier (BBB) serves as a protective barrier for the central nervous system (CNS) against drugs that enter the bloodstream. The BBB is a key clinical barrier in the treatment of CNS illnesses because it restricts drug entry into the brain. To bypass this barrier and release relevant drugs into the brain matrix, nanotechnology-based delivery systems have been developed. Given the unstable nature of NPs, an appropriate amount of a biocompatible polymer coating on NPs is thought to have a key role in reducing cellular cytotoxicity while also boosting stability. Human serum albumin (HSA), poly (lactic-co-glycolic acid) (PLGA), Polylactide (PLA), poly (alkyl cyanoacrylate) (PACA), gelatin, and chitosan are only a few of the significant polymers mentioned. In this review article, we categorized polymer-coated nanoparticles from basic to complex drug delivery systems and discussed their application as novel drug carriers to the brain.

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Protein-Excipient Interactions Evaluated via Nuclear Magnetic Resonance Studies in Polysorbate-Based Multidose Protein Formulations: Influence on Antimicrobial Efficacy and Potential Study Approach

Cited by 14 publications

References 8 publications

Albumin displacement at the air–water interface by Tween (Polysorbate) surfactants

Albumin displacement at the air–water interface by Tween (Polysorbate) surfactants

Multi-Dose Formulation Development for a Quadrivalent Human Papillomavirus Virus-Like Particle-Based Vaccine: Part I - Screening of Preservative Combinations

Management of Brain Cancer and Neurodegenerative Disorders with Polymer-Based Nanoparticles as a Biocompatible Platform

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