Protein Expression Level of Skin Wrinkle-Related Factors in Hairless Mice Fed Hyaluronic Acid

Abstract

The aim of this study was to evaluate the wrinkle improving effect of hyaluronic acid intakes. Wrinkles were induced by exposing the skin of hairless mice to ultraviolet B (UVB) irradiation for 14 weeks. Hyaluronic acid was administered to the mice for 14 weeks including 4 weeks before experiments. Skin tissue was assayed by enzyme-linked immunosorbent assay to determine protein expression of wrinkle-related markers. The group supplemented with high concentrations of hyaluronic acid appeared significantly better than control group for collagen, matrix metalloproteinase 1, interleukin (IL)-1β, and IL-6 assay. Transforming growth factor-β1 (TGF-β1) and hyaluronic acid synthase 2 (HAS-2) were not shown to be significantly different. In conclusion, hyaluronic acid administration regulated expression levels of proteins associated with skin integrity, and improved the wrinkle level in skin subjected to UVB irradiation.

Hyaluronan: A key player or just a bystander in skin photoaging?

“…A further study determined that the administration of HA (the MW of the HA was not specified) to photoaged mice served to regulate the expression levels of proteins associated with skin integrity, also improved the wrinkle level in UVB photoaged skin. The HA‐treated mice evinced significantly more favourable collagen, MMP1, IL‐1β and IL‐6 protein expression patterns; the TGF‐β1 and HAS‐2 remained practically unaltered 131 …”

“…The HA-treated mice evinced significantly more favourable collagen, MMP1, IL-1β and IL-6 protein expression patterns; the TGF-β1 and HAS-2 remained practically unaltered. 131 Several potential mechanisms exist with respect to how ingested exogenous HA affects the properties of the skin. Firstly, p.o.-applied HA crosses the intestinal barrier and is translocated to the skin.…”