Protein expression profiling identifies maspin and stathmin as potential biomarkers of adenoid cystic carcinoma of the salivary glands

Nakashima, Daisuke; Uzawa, Katsuhiro; Kasamatsu, Atsushi; Koike, Hirofumi; Endo, Yutaka; Saito, Kengo; Hashitani, Susumu; Numata, Tsutomu; Urade, Masahiro; Tanzawa, Hideki

doi:10.1002/ijc.21318

Cited by 48 publications

(22 citation statements)

References 52 publications

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“…The loss of maspin has been reported with poor prognosis in breast cancers, oral squamous cell carcinoma, lung, and prostate carcinoma (7,13). In accordance with previous studies (20,21), the results of this study showed inverse significant association between maspin expression and histologic grade. No significant correlation was found between maspin expression and lymph node metastasis, which was similar to Ghazy et al's study (22), but in contrast to the findings of Schwartz et al (23).…”

Section: Discussionsupporting

confidence: 91%

Prognostic Value of P63, Maspin, and MMP-2 Expression in Salivary Gland Adenoid Cystic Carcinoma

et al. 2018

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Background: As one of the most malignant neoplasm of salivary glands, adenoid cystic carcinoma (ACC) displays epithelial and myoepithelial differentiation. The myoepithelial cells seem to act as tumor-suppressor via their secretory properties. Objectives: The present study was designed to evaluate the prognostic significance of p63 (myoepithelial cell marker), maspin, and MMP-2 expression in samples of ACC. Methods: P63, maspin, and MMP-2 expression were examined immunohistochemically in 32 cases with ACC. The relationship between markers expression and clinicopathologic data was assessed, using non-parametric tests and t test. Results: P63 and maspin expression demonstrated inverse significant correlation with histologic grade, respectively (P = 0.045, P = 0.019). Indeed, association between P63 and maspin expression was observed (r = 0.588, P < 0.001). Correlation between tumor size and lymph node metastasis was also found (P = 0.016). Conclusions: According to the correlation of P63 and maspin expression with histologic grade, as a prognostic determinant of ACC, P63 and maspin can be useful markers for predicting biologic behavior of ACC, which may lead to new treatment modality in salivary gland tumors containing myoepithelial cell. Furthermore, tumor size can be considered as an indicator of lymph node metastasis.

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Section: Discussionsupporting

confidence: 91%

Prognostic Value of P63, Maspin, and MMP-2 Expression in Salivary Gland Adenoid Cystic Carcinoma

et al. 2018

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“…Therefore, the function of glandular epithelium is important for the establishment of pregnancy. Since stathmin localization has been reported in a number of secretory glands, including salivary gland (Nakashima et al 2006), mammary gland Figure 8 Effect of stathmin knockdown on the proliferation of cultured endometrial stromal cells. Stromal cells were transfected with stathmin siRNA or an irrelevant control siRNA (stathmin siRNA(K)) and cultured for 24 and 48 h, after which the proliferation rate of the cells was assayed by using the WST-1 reagent (B).…”

Section: Discussionmentioning

confidence: 99%

Expression of stathmin in human uterus and decidualizing endometrial stromal cells

Tamura¹,

Yoshie²,

Nishi³

et al. 2006

Reproduction

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The cytosolic phosphoprotein stathmin is upregulated at the site of embryo implantation in the rodents. However, stathmin expression in the human uterus has not yet been investigated. The distribution of uterine and placental stathmin was analyzed by immunohistochemistry, while stathmin mRNA expression was detected in endometrial tissues by the reverse transcriptase-PCR. Cultured endometrial stromal cells were used to investigate whether stathmin plays a role in decidualization. Stathmin is expressed specifically in the glandular epithelium and the stromal cells of human endometrial tissue. It is also expressed by cytotrophoblasts and extravillous trophoblasts, but not by syncytiotrophoblasts or decidual tissues during the first trimester of pregnancy. When stromal cells isolated from normal endometrial tissues were cultured and stimulated to decidualize by progesterone (P4) plus estrogen or dibutyryl cyclic 3 0 ,5 0 -AMP, their total and phosphorylated stathmin levels decreased. Knocking down stathmin expression in the cultured stromal cells using small interfering RNA, before the cells were exposed to the decidualizing agents, significantly suppressed decidualization, as indicated by the decreased expression of IGF-binding protein-1 and prolactin. Stathmin is differently expressed in human endometrial and placental cells and may participate in the decidualization of endometrial stromal cells.

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“…We did proteomic profiling of NOKs and OSCCderived cell lines (HSC-2 and HSC-3) using fluorescent 2D-DIGE and MALDI-TOF-MS fingerprinting in triplicate as described previously (9). Each protein extract (50 Ag) was used for the 2D-DIGE analysis.…”

Section: Methodsmentioning

confidence: 99%

Lin-7C/VELI3/MALS-3: An Essential Component in Metastasis of Human Squamous Cell Carcinoma

Onda

Uzawa

Nakashima³

et al. 2007

Cancer Research

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Using proteomic selection, functional verification, and clinical validation, we identified specific down-regulation of Lin-7C/ VELI3/MALS-3 (Lin-7C), which marks oral squamous cell carcinoma (OSCC) metastasis. Despite a rarity of sequence variations in the Lin-7C gene in both primary OSCC and OSCCderived cells, a high prevalence of hypermethylation was detected in the CpG island region that strongly correlated with its down-regulation. Inducible Lin-7C mRNA by experimental demethylation was found in all OSCC cells tested. Overexpression of the Lin-7C gene in an OSCC cell clone does not contribute to underproliferation but results in a noninvasive phenotype with elevated b-catenin expression. Experimental metastases in multiple organs of immunodeficient mice were inhibited in cells expressing Lin-7C. Finally, the Lin-7C expression status in primary tumors afforded significantly (P < 0.001) high accuracy for predicting lymph node metastasis. These results establish Lin-7C as a novel target of early detection, prevention, and therapy for OSCC metastasis.

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Protein expression profiling identifies maspin and stathmin as potential biomarkers of adenoid cystic carcinoma of the salivary glands

Cited by 48 publications

References 52 publications

Prognostic Value of P63, Maspin, and MMP-2 Expression in Salivary Gland Adenoid Cystic Carcinoma

Prognostic Value of P63, Maspin, and MMP-2 Expression in Salivary Gland Adenoid Cystic Carcinoma

Expression of stathmin in human uterus and decidualizing endometrial stromal cells

Lin-7C/VELI3/MALS-3: An Essential Component in Metastasis of Human Squamous Cell Carcinoma

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