Protein Hydrolysate Fractions from Sea Cucumber (<i>Isostichopus badionotus</i>) Inhibit Angiotensin-Converting Enzyme

Chim-Chi, Yasser; Olivera‐Castillo, Leticia; Betancur‐Ancona, David; Chel‐Guerrero, Luis

doi:10.1080/10498850.2015.1080775

Cited by 7 publications

(9 citation statements)

References 33 publications

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“…After that, the hydrolysis reactions followed a linear trend until the end of each experiment, the result of either a limitation on the available cutting sites, enzyme denaturation and/or product inhibition [ 74 ]. These results were similar to those reported for Stichopus horrens [ 123 ], Actinopyga lecanora [ 126 ], and Isostichopus badionotus [ 17 ].…”

Section: Resultssupporting

confidence: 91%

“…Among marine benthic invertebrates, Holothurians (commonly known as sea cucumbers) have received particular attention due to the presence of biomolecules that have health benefits such as: (1) chondroitin sulfates [ 4 ]; (2) fucan sulfates [ 5 ]; (3) polysaccharides [ 6 ]; (4) triterpene glycosides (saponins) [ 7 ]. These four biomolecule types have applications in the pharmaceutical industry due to their broad panel of bioactivities, such as antibacterial, antifungal, antiprotozoal, anti-inflammatory, anticoagulant, antitumor, antioxidant, and antiviral activities [ 8 , 9 , 10 ]; (5) long-chain polyunsaturated fatty acids (PUFAs) that play an essential role in metabolic activities [ 11 ], coronary heart diseases, arthritis, wound healing, and blood clotting [ 12 , 13 , 14 ], and (6) peptides [ 15 ] which exhibit angiotensin-converting enzyme (ACE) inhibition [ 16 , 17 , 18 ]. Peptides’ biological activities decrease sometimes due to digestion or poor absorption [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Physicochemical Properties and Liposomal Formulations of Hydrolysate Fractions of Four Sea Cucumbers (Holothuroidea: Echinodermata) from the Northwestern Algerian Coast

et al. 2020

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To promote the nutritional and pharmacological values of four sea cucumber species (Holothuria poli, H. tubulosa, H. arguinensis, and H. sanctori), harvested from the Algerian coast, we aimed to study their proximate composition, fatty acid profile and angiotensin-converting enzyme (ACE) inhibitory activity. Their phospholipids were also used to elaborate nanoliposomes and to encapsulate peptides obtained from the same source. After the physico-chemical characterization of nanoliposomes and peptides, in vitro analyses were realized. The four holothurian species showed a high amount of protein (49.26–69.34%), and an impressive lipid profile of 27 fatty acids, mainly composed of polar fatty acids (91.16–93.85%), with a high polyunsaturated fatty acids (PUFA) content (50.90–71.80%), particularly eicosapentaenoic acid (EPA) (5.07–8.76%) and docosahexaenoic acid (DHA) (4.86–7.25%). A high phospholipids amount was also found (55.20–69.85%), mainly composed of phosphatidylcholine (PC) (51.48–58.56%). Their peptide fractions exhibited a high ACE inhibitory activity (IC50 0.30 to 0.51 mg/mL). The results also showed that the nanoliposomes do not induce cytotoxicity and cell death in human MSCs and no perturbation of proliferation for all the times and the tested concentrations, as well as the combined nanoliposomes and hydrolysates (HTS) at a concentration of 0.1 mg/mL. All four sea cucumbers show potential as a new source for omega-3, omega-6, and bioactive peptides.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Physicochemical Properties and Liposomal Formulations of Hydrolysate Fractions of Four Sea Cucumbers (Holothuroidea: Echinodermata) from the Northwestern Algerian Coast

et al. 2020

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“…In another study, Alcalase/Protease produced peptides with angiotensin I-converting enzyme inhibitory activity from skate (Okamejei kenojei) skin gelatin, which were able to reduce the systolic blood pressure in spontaneously hypertensive rats [542]. Also, pepsinpancreatin and Alcalase-Flavorzyme sequential systems were used to prepare hydrolysates with angiotensin I-converting enzyme inhibitory capacity from sea cucumber (Isostichopus badionotus) [543]. It was found that the Alcalase-Flavourzyme system produced hydrolysates with the highest degree of hydrolyses and angiotensin I-converting enzyme inhibitory action (86%) [543].…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

“…Also, pepsinpancreatin and Alcalase-Flavorzyme sequential systems were used to prepare hydrolysates with angiotensin I-converting enzyme inhibitory capacity from sea cucumber (Isostichopus badionotus) [543]. It was found that the Alcalase-Flavourzyme system produced hydrolysates with the highest degree of hydrolyses and angiotensin I-converting enzyme inhibitory action (86%) [543].…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Use of Alcalase in the production of bioactive peptides: A review

Tacias-Pascacio

Morellon‐Sterling

Siar

et al. 2020

International Journal of Biological Macromolecules

227

114

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…Additionally, enzymatic activity can be protected and enzymatic hydrolysis efficiency improved by removing fat (using organic reagents like ethanol), 6 heavy metals (using EDTA), 26 and salts (by dialysis). 30 In the process of enzymatic hydrolysis, enzyme type plays a key role in the preparation of SCP as it determines the optimal temperature and pH on the basis of the bioactivity requirements. The generation of SCP by enzymatic hydrolysis includes in vivo digestion and in vitro production.…”

Section: Production Of Sea Cucumber Peptidesmentioning

confidence: 99%

Production of Bioactive Peptides from Sea Cucumber and Its Potential Health Benefits: A Comprehensive Review

Sun

Liu

et al. 2022

J. Agric. Food Chem.

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Bioactive peptides from food have been widely studied due to their potential applications as functional foods and pharmaceuticals. Sea cucumber, a traditional tonic food, is characterized by high protein and low fat, thereby substrates are being studied to release sea cucumber peptides (SCPs). Although recent studies have shown that SCPs have various bioactive functions, there is no literature reviewing the development status of SCPs. In this review, we summarized the production of SCPs, including their purification and identification, then mainly focused on the comprehensive potential health benefits of SCP in vivo and in vitro, and finally discussed the challenge facing the development of SCPs. We found that SCPs have well-documented health benefits due to their antioxidation, anti-diabetes, ACE inhibitory, immunomodulatory, anti-cancer, anti-fatigue, anti-aging, neuroprotection, micromineral-chelating, etc. However, the structure−activity relationships of SCPs and the functional molecular mechanisms underlying their regulation in vivo need further investigation. Research on the safety of SCP and its potential regulation mechanism will contribute to transferring these findings into commercial applications. Hopefully, this review could promote the development and application of SCPs in further investigation and commercialization.

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Protein Hydrolysate Fractions from Sea Cucumber (Isostichopus badionotus) Inhibit Angiotensin-Converting Enzyme

Cited by 7 publications

References 33 publications

Physicochemical Properties and Liposomal Formulations of Hydrolysate Fractions of Four Sea Cucumbers (Holothuroidea: Echinodermata) from the Northwestern Algerian Coast

Physicochemical Properties and Liposomal Formulations of Hydrolysate Fractions of Four Sea Cucumbers (Holothuroidea: Echinodermata) from the Northwestern Algerian Coast

Use of Alcalase in the production of bioactive peptides: A review

Production of Bioactive Peptides from Sea Cucumber and Its Potential Health Benefits: A Comprehensive Review

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