Protein identification and localization using mass spectrometry and staining tests in cross-sections of polychrome samples

Kučková, Štěpánka; Sandu, Irina Crina Anca; Crhova, Michaela; Hynek, Radovan; Fogaš, Igor; Schäfer, Stephan

doi:10.1016/j.culher.2012.03.004

Cited by 46 publications

(26 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been applied more recently to the analysis of proteins in ancient bones (102,103) and eggshells (104), where it allows the identification of species, and has been used to analyse proteinaceous and lipid binders in works of art and wall plasters (105)(106)(107)(108). It has been used to analyze proteinaceous glue from a wooden building (109), to study the photo-degradation of terpenoid varnishes (110), and to assess the condition and penetration of polyethylene glycol in preserved ships (111).…”

Section: New Mass Spectrometric Techniquesmentioning

confidence: 99%

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Steele

2013

ACS Symposium Series

View full text Add to dashboard Cite

The analysis of organic residues from archaeological materials has become increasingly important to our understanding of ancient diet, trade and technology. Residues from diverse contexts have been retrieved and analysed from the remains of food, medicine and cosmetics to hafting material on stone arrowheads, pitch and tar from shipwrecks, and ancient manure from soils. Research has brought many advances in our understanding of archaeological, organic residues over the past two decades. Some have enabled very specific and detailed interpretations of materials preserved in the archaeological record. However there are still areas where we know very little, like the mechanisms at work during the formation and preservation of residues, and areas where each advance produces more questions rather than answers, as in the identification of degraded fats. This chapter will discuss some of the significant achievements in the field over the past decade and the ongoing challenges for research in this area.1341280_File000001_20903114.docPrinted 7/19/2013 2

show abstract

Section: New Mass Spectrometric Techniquesmentioning

confidence: 99%

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Steele

2013

ACS Symposium Series

View full text Add to dashboard Cite

show abstract

“…[1] Protein-based materials have been used extensively in cultural heritage objects,b oth by artists,a spaint binders for example,o rf or conservation as consolidants or adhesives.Their identification can provide insight into artistic technique or history of the object and can be helpful in defining display and storage conditions,a sw ell as conservation protocols.Proteomics,orpalaeoproteomics when applied to ancient samples,h as already been successfully used for studying proteins in samples from works of art, providing accurate information about the nature of the material, its biological source,a nd degradation status. [2][3][4][5][6][7][8][9][10][11] Herein we present the first attempt, to our knowledge,t oi nvestigate in detail multiple post-translational modifications (PTMs) of unknown proteinaceous layers applied to aw all painting.…”

mentioning

confidence: 99%

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

et al. 2018

View full text Add to dashboard Cite

Ahead of display, a non‐original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post‐translational modifications detected several photo‐oxidation products, which suggest that the egg experienced prolonged exposure to UV light and was likely applied long before the glue layer. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR‐FTIR in a cross‐section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.

show abstract

“…Their identification can provide insight into artistic technique or history of the object and can be helpful in defining display and storage conditions, as well as conservation protocols. Proteomics, or palaeoproteomics when applied to ancient samples, has already been successfully used for studying proteins in samples from works of art, providing accurate information about the nature of the material, its biological source, and degradation status . Herein we present the first attempt, to our knowledge, to investigate in detail multiple post‐translational modifications (PTMs) of unknown proteinaceous layers applied to a wall painting.…”

Section: Figurementioning

confidence: 55%

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

et al. 2018

View full text Add to dashboard Cite

Ahead of display, anon-original layer was observed on the surface of af ragment of aw all painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duckegg white.Analysis of post-translational modifications detected several photooxidation products,w hichs uggest that the egg experienced prolonged exposure to UV light and was likely applied long before the glue layer.Additionally,glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with acarbohydrate material such as starch,identified by ATR-FTIR in ac ross-section of as ample taken from the painting.P alaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.

show abstract

Protein identification and localization using mass spectrometry and staining tests in cross-sections of polychrome samples

Cited by 46 publications

References 20 publications

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

Contact Info

Product

Resources

About