Protein–inorganic hybrid system for efficient his-tagged enzymes immobilization and its application in <scp>l</scp>-xylulose production

Patel, Sanjay; Otari, Sachin V.; Kang, Yun Chan; Lee, Jung-Kul

doi:10.1039/c6ra24404a

Cited by 93 publications

(45 citation statements)

References 46 publications

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“…There is a wide range of enzyme immobilization techniques involving physical entrapment or chemical interactions (either covalent or non-covalent) between the enzyme and the support [9,[12][13][14][15][16]. These techniques have been applied to many enzymes, including -galactosidases [5,9,12,[17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Development of enzymatically-active bacterial cellulose membranes through stable immobilization of an engineered β-galactosidase

Estevinho

Samaniego

Talens-Perales

et al. 2018

International Journal of Biological Macromolecules

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Enzymatically-active bacterial cellulose (BC) was prepared by non-covalent immobilization of a hybrid enzyme composed by a β-galactosidase from Thermotoga maritima (TmLac) and a carbohydrate binding module (CBM2) from Pyrococcus furiosus. TmLac-CBM2 protein was bound to BC, with higher affinity at pH 6.5 than at pH 8.5 and with high specificity compared to the non-engineered enzyme. Both hydrated (HBC) and freeze-dried (DBC) bacterial cellulose showed equivalent enzyme binding efficiencies. Initial reaction rate of HBC-bound enzyme was higher than DBC-bound and both of them were lower than the free enzyme. However, enzyme performance was similar in all three cases for the hydrolysis of 5% lactose to a high extent. Reuse of the immobilized enzyme was limited by the stability of the β-galactosidase module, whereas the CBM2 module provided stable attachment of the hybrid enzyme to the BC support, after long incubation periods (3 h) at 75 °C.

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Section: Introductionmentioning

confidence: 99%

Development of enzymatically-active bacterial cellulose membranes through stable immobilization of an engineered β-galactosidase

Estevinho

Samaniego

Talens-Perales

et al. 2018

International Journal of Biological Macromolecules

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“…EY (%) = 100 9 protein concentration of nanohybrids/ initial protein concentration (mg/mL) [11]. The immobilized enzymes were characterized by scanning electron microscopy (SEM), confocal microscopy, and energy dispersive x-ray spectroscopy (EDX).…”

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confidence: 99%

Synthesis of Protein-Inorganic Nanohybrids with Improved Catalytic Properties Using Co3(PO4)2

et al. 2017

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In the present study, a method for easy and rapid synthesis of lipase nanohybrids was evaluated using cobalt chloride as an encapsulating agent. The synthesized nanohybrids exhibited higher activity (181%) compared to free lipase and improved catalytic properties at higher temperature and in harsh conditions. The nanohybrids retained 84% of their residual activity at 25 °C after 10 days. In addition, these nanohybrids also exhibited high storage stability and reusability. Collectively, the synthesis of carrier-free immobilized biocatalysts was performed rapidly within 24 h at 4 °C. Their high reusability and catalytic activities highlight the broad applicability of this method for catalysis in organic and aqueous media.

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“…Their result showed that for the synthesis of enzyme hNFs, the relative activity of the obtained immobilized enzyme was greatly decreased from 246 AE 21% (24 h incubation) to 14.4 AE 2.1% (3 days incubation) for L-arabinitol 4-dehydrogenase from Hypocrea jecorina and from 144 AE 16% (24 h incubation) to 17.1 AE 12% (3 days incubation) for NADH oxidase from Streptococcus pyogenes. 15,43 Characterization of tfdB-JLU-hNFs…”

Section: Growth Of Tfdb-jlu-hnfsmentioning

confidence: 99%

Self-assembled 2,4-dichlorophenol hydroxylase-inorganic hybrid nanoflowers with enhanced activity and stability

et al. 2018

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Protein–inorganic hybrid system for efficient his-tagged enzymes immobilization and its application in l-xylulose production

Abstract: Efficient his-tagged recombinant enzyme nanoflowers were synthesized and used for rare sugar production under co-factor regeneration conditions.

Cited by 93 publications

References 46 publications

Development of enzymatically-active bacterial cellulose membranes through stable immobilization of an engineered β-galactosidase

Development of enzymatically-active bacterial cellulose membranes through stable immobilization of an engineered β-galactosidase

Synthesis of Protein-Inorganic Nanohybrids with Improved Catalytic Properties Using Co3(PO4)2

Self-assembled 2,4-dichlorophenol hydroxylase-inorganic hybrid nanoflowers with enhanced activity and stability

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