Protein Interactions and Nanomaterials: A Key Role of the Protein Corona in Nanobiocompatibility

Ânia, Micaelo; Emilio, Rodriguez Cerezo; Millán, Ángel; Góngora, Rafael; Fuentes, Manuel

doi:10.5772/intechopen.75501

Cited by 5 publications

(8 citation statements)

References 91 publications

(134 reference statements)

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“…If opsonins such as immunoglobulin G (IgG) are present on the protein corona, the NPs will be marked as a xenobiotic and eliminated by the reticuloendothelial system (RES), also called mononuclear phagocyte system, 124,125 composed mainly of blood monocytes and tissue macrophages, which shuttle the nanoparticles out of the circulation to the spleen, liver, or bone marrow. To avoid this, the particle surfaces are modified to acquire stealth capabilities, in other words, to render them "invisible" to the RES.…”

Section: Passive and Active Targetingmentioning

confidence: 99%

“…It is a well-known fact that when NPs are injected in the bloodstream, they will immediately interact with serum proteins, leading to the formation of a protein layer called protein corona. − The type and composition of the protein corona around the NPs is influenced by their physicochemical properties (size, shape, composition, surface charges) and the time of exposure. If opsonins such as immunoglobulin G (IgG) are present on the protein corona, the NPs will be marked as a xenobiotic and eliminated by the reticuloendothelial system (RES), also called mononuclear phagocyte system, , composed mainly of blood monocytes and tissue macrophages, which shuttle the nanoparticles out of the circulation to the spleen, liver, or bone marrow. To avoid this, the particle surfaces are modified to acquire stealth capabilities, in other words, to render them “invisible” to the RES .…”

Section: Medical Imagingmentioning

confidence: 99%

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Molecularly Imprinted Polymers: Antibody Mimics for Bioimaging and Therapy

2020

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Molecularly imprinted polymers (MIPs) are tailor-made chemical receptors that recognize and bind target molecules with a high affinity and selectivity. MIPs came into the spotlight in 1993 when they were dubbed “antibody mimics,” and ever since, they have been widely studied for the extraction or trapping of chemical pollutants, in immunoassays, and for the design of sensors. Owing to novel synthesis strategies resulting in more biocompatible MIPs in the form of soluble nanogels, these synthetic antibodies have found favor in the biomedical domain since 2010, when for the first time, they were shown to capture and eliminate a toxin in live mice. This review, covering the years 2015–2020, will first describe the rationale behind these antibody mimics, and the different synthesis methods that have been employed for the preparation of MIPs destined for in vitro and in vivo targeting and bioimaging of cancer biomarkers, an emerging and fast-growing area of MIP applications. MIPs have been synthesized for targeting and visualizing glycans and protein-based cell receptors overexpressed in certain diseases, which are well-known biomarkers for example for tumors. When loaded with drugs, the MIPs could locally kill the tumor cells, making them efficient therapeutic agents. We will end the review by reporting how MIPs themselves can act as therapeutics by inhibiting cancer growth. These works mark a new opening in the use of MIPs for antibody therapy and even immunotherapy, as materials of the future in nanomedicine.

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Section: Passive and Active Targetingmentioning

confidence: 99%

Section: Medical Imagingmentioning

confidence: 99%

Molecularly Imprinted Polymers: Antibody Mimics for Bioimaging and Therapy

2020

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“…This may lead to NPs aggregates and more prone to phagocytic uptake, or the physicochemical properties may change implicating changes in their kinetic behavior either limiting their effect or intensifying their toxicity (Bello and Warheit 2017). Further details about the protein corona and its effects on NP activity can be found in these sources (Monopoli et al 2011;Vilanova et al 2016;Ânia et al 2018;Nierenberg, Khaled, and Flores 2018;Cao et al 2019). A stealth/shielding technique for NPs are currently designed to limit the effect of protein corona (Vij et al 2010).…”

Section: Inhaled Nanoparticle Adme Kinetics and Toxicitymentioning

confidence: 99%

Toxicological assessment of nanoparticle interactions with the pulmonary system

2019

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“…It also assesses the insights we have had so far as a "reality check" to help researchers in making more "targeted" efforts in this area. For more information on this subject, some excellent recent resources are an overview by Ke et al (2017), 23 a book chapter by A ̂nia et al ( 2018) 24 and a recent book devoted exclusively to protein corona (Kumar and Dhawan 2019). 25…”

Section: ■ Introductionmentioning

confidence: 99%

How Corona Formation Impacts Nanomaterials as Drug Carriers

Gupta

Roy

2020

Mol. Pharmaceutics

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As drugs/drug carriers, upon encountering physiological fluids, nanoparticles adsorb biological molecules almost immediately to form a biocorona, which is often simply called a corona. Once the corona is formed, it dictates the subsequent fate of the drug nanoparticle as a therapeutic agent. Protein adsorption on micron-size or even bigger particles was originally described by the Vroman effect. It has served as a useful framework to understand the corona formation. Proteins that are irreversibly adsorbed on nanoparticles form what is called a hard corona. Beyond that is the exchangeable population of proteins, which constitute the dynamic structure called a soft corona. More than the abundance, the affinity of the proteins toward the nanoparticles decides which ones end up in the corona. For example, the more common serum albumin, which is deposited initially, is displaced by fibrinogen, which has a higher affinity for gold nanoparticles. The curvature of the particle is a crucial parameter with bigger particles generally able to bind a more diverse population of proteins from the physiological milieu. The earlier perception of the corona formation being a challenge for drug targeting, etc. has been turned into an opportunity by engineering corona to manipulate properties like circulating half-lives, capacity to evade the immune system, and targeting or even overcoming the blood−brain barrier. The most commonly used techniques for particle characterization, including dynamic light scattering (DLS), differential sedimentation centrifugation, transmission electron microscopy (TEM), and SDS-PAGE, have been adopted to study corona formation in the past. Many newer tools, for example, a combination of capillary electrophoresis with mass spectrometry, are being used to study the corona composition. The comparison of interlaboratory results is a problem because of the lack of standard protocols. This has hindered the ability to obtain more precise information about the corona composition. That, in turn, affects our prospects to use nanoparticles as drugs/drug carriers. This overview is an attempt to assess our understanding of corona formation critically and to outline the complexities involved in gaining precise information. The discussion is largely focused on findings of the last year or so.

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Protein Interactions and Nanomaterials: A Key Role of the Protein Corona in Nanobiocompatibility

Cited by 5 publications

References 91 publications

Molecularly Imprinted Polymers: Antibody Mimics for Bioimaging and Therapy

Molecularly Imprinted Polymers: Antibody Mimics for Bioimaging and Therapy

Toxicological assessment of nanoparticle interactions with the pulmonary system

How Corona Formation Impacts Nanomaterials as Drug Carriers

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