Protein interactions in covalently attached dextran layers

Piehler, Jacob; Brecht, Andreas; Hehl, Karin; Gauglitz, Günter

doi:10.1016/s0927-7765(99)00046-6

Cited by 109 publications

(104 citation statements)

References 33 publications

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“…Previous studies aiming to explore antifouling properties of sugars include, for example, a study by Morra and Cassineli (1999) showing that highly hydrated hydrophilic polysaccharides could prevent mammalian cell adhesion and reduce bacterial adhesion. The non-charged natural polysaccharide dextran forms highly hydrated hydrogels which show very low non-specific interactions with proteins (Piehler et al 1999). Luk et al (2000) found a monosaccharide, mannitol-terminated alkylthiol, to be resistant to both protein adsorption and cell attachment.…”

Section: Introductionmentioning

confidence: 99%

Antifouling properties of oligo(lactose)-based self-assembled monolayers

et al. 2015

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The antifouling properties of oligo(lactose)-based self-assembled monolayers (SAMs), using four different proteins, zoospores of the green alga Ulva linza and cells of the diatom Navicula incerta, were investigated. The SAM-forming alkylthiols, which contained 1, 2 or 3 lactose units, showed significant variation in antifouling properties, with no differences in wettability.Non-specific adsorption of albumin and pepsin was low on all surfaces. Adsorption of lysozyme and fibrinogen decreased with increasing number of lactose units in the SAM, in agreement with the generally observed phenomenon that thicker hydrated layers provide higher barriers to protein adsorption. Settlement of spores of U. linza followed an opposite trend, being greater on the bulkier, more hydrated SAMs. These SAMs are more ordered for the larger saccharide units, and it is therefore hypothesized that the degree of order, and differences in crystallinity or stiffness between the surfaces, is an important parameter regulating spore settlement on these surfaces.

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Section: Introductionmentioning

confidence: 99%

Antifouling properties of oligo(lactose)-based self-assembled monolayers

et al. 2015

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“…Meanwhile, dextran, an a(1-6)-linked glycan, is nontoxic, water soluble and neutral, with multiple reactive sites available for subsequent functionalization for targeting ligands. Importantly, dextran has demonstrated outstanding properties against nonspecific protein adhesion by our group [25,26] and others [27][28][29]. Finally, dextran coated delivery systems have shown both reduced opsonisation by proteins in the medium [30] and reduced nonspecific hydrophobic interactions between the carrier and cell membranes.…”

Section: Discussionmentioning

confidence: 99%

Cellular Uptake and Intracellular Cargo Release From Dextran Based Nanogel Drug Carriers

Ferrer

Sobolewski²,

Composto

et al. 2013

Journal of Nanotechnology in Engineering and Medicine

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Nanogels (NG) hold great promise as a drug delivery platform. In this work, we examine the potential of lysozyme-dextran nanogels (LDNG) as drug carriers in vitro using two cell lines: a model target tissue, human umbilical cord vein endothelial cells (HUVEC) and a model of the mononuclear phagocyte system (phorbol 12-myristate 13-acetate (PMA)-stimulated THP-1 cells). The LDNG ($100 nm) were prepared with rhodaminelabel dextran (LRDNG) via Maillard reaction followed by heat-gelation reaction and were loaded with a fluorescent probe, 5-hexadecanoylaminofluorescein (HAF), as a mock drug. Epifluorescence microscopy confirmed rapid uptake of LRDNG by HUVEC. Although LysoTracker Green staining indicated a lysosomal fate for LRDNG, the mock drug cargo (HAF) diffused extensively inside the cell within 15 min. Flow cytometry and confocal microscopy indicated slow uptake of LRDNG in PMA-stimulated THP-1 cells, with only 41% of cells containing LRDNG after 24 h exposure. Finally, 24 h exposure to LRDNG did not affect the viability of either cell type at the dose studied (20 lg/ml). At a higher dose (200 lg/ml), LRDNG resulted in a marked loss of viability of HUVEC and THP-1, measuring 30% and 38%, respectively. Collectively, our results demonstrate the great potential of LRDNG as a drug delivery platform, combining simple production, rapid uptake and cargo release in target cells with "stealth" properties and low cytotoxicity.

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“…The ability of a microbial population to degrade pollutants in an environment can be increased by stimulating the indigenous microorganisms (El Fantroussi & Agathos 2005). The role of indigenous bacteria in biodegrading petroleum pollution was first documented by Piehler et al (1999). Iwabuchi et al (1998) reported that indigenous bacteria can out-compete artificially introduced strains in several bioremediation investigations.…”

Section: Introductionmentioning

confidence: 99%