Protein interactions with subcutaneously implanted biosensors

Gifford, Raeann; Kehoe, Joseph J.; Barnes, Sandra L.; Kornilayev, Boris A.; Alterman, Michail A.; Wilson, George S.

doi:10.1016/j.biomaterials.2005.11.033

Cited by 109 publications

(82 citation statements)

References 38 publications

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“…In order to study the biofouling properties of the samples they were immersed in BSA solutions for 24 hours at room temperature to compare the current responses for FcMeOH in H2SO4 or PBS before and after exposure to possibly fouling solutions. It has been previously shown that serum albumin adsorbs on Pt containing biosensor surface [49] which justifies the use of BSA as a model solution. Samples were also immersed in deionized water for reference.…”

Section: Biofoulingmentioning

confidence: 99%

Electrochemical detection of hydrogen peroxide on platinum-containing tetrahedral amorphous carbon sensors and evaluation of their biofouling properties

Tujunen

Kaivosoja

Protopopova

et al. 2015

Materials Science and Engineering: C

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Hydrogen peroxide is the product of various enzymatic reactions, and is thus typically utilized as the analyte in biosensors. However, its detection with conventional materials, such as noble metals or glassy carbon, is often hindered by slow kinetics and biofouling of the electrode. In this study electrochemical properties and suitability to peroxide detection as well as ability to resist biofouling of Pt-doped ta-C samples were evaluated. Pure ta-C and pure Pt were used as references. According to the results presented here it is proposed that combining ta-C with Pt results in good electrocatalytic activity towards H2O2 oxidation with better tolerance towards aqueous environment mimicking physiological conditions compared to pure Pt. In biofouling experiments, however, both the hybrid material and Pt were almost completely blocked after immersion in protein-containing solutions and did not produce any peaks for ferrocenemethanol oxidation or reduction. On the contrary, it was still possible to obtain clear peaks for H2O2 oxidation with them after similar treatment. Moreover, quartz crystal microbalance experiment showed less protein adsorption on the hybrid sample compared to Pt which is also supported by the electrochemical biofouling experiments for H2O2 detection.

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Section: Biofoulingmentioning

confidence: 99%

Electrochemical detection of hydrogen peroxide on platinum-containing tetrahedral amorphous carbon sensors and evaluation of their biofouling properties

Tujunen

Kaivosoja

Protopopova

et al. 2015

Materials Science and Engineering: C

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“…4,5 Other concerns such as direct long-term integration with the host have not been completely solved. [6][7][8][9] The primary challenge affecting subcutaneous implanted glucose sensors as well as any implanted sensing device has been overcoming the host foreign body response while retaining proper function, e.g., resistance to signal drift and calibration maintenance. 10,11 The immense tissue-integration challenges associated with in vivo glucose measurements have precluded the long-term implantation (1−4 weeks) of glucose sensors for continuous glucose monitoring.…”

Section: Introductionmentioning

confidence: 99%

Microdialysis Sampling Extraction Efficiency of 2-Deoxyglucose: Role of Macrophages in Vitro and in Vivo

Mou

Stenken

2006

Anal. Chem.

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Macrophages are a class of inflammatory cells believed to direct the outcome of device biocompatibility. Despite their relevance to implanted in vivo devices, particularly implanted glucose sensors, few studies have attempted to elucidate how these cells affect device performance. Microdialysis sampling probes were used to determine glucose uptake alterations in the presence of resting and activated macrophages in vitro. Significant differences for 2-DG relative recovery at 1.0 μL/min were observed between resting (74 ± 7%, n=18) and lipopolysaccharide (LPS) (1 μg/mL) activated (56 ± 6%, n=18) macrophages in culture which had 2-DG spiked into the media (p<0.005). To establish if in vitro characterization could be correlated to in vivo studies, microdialysis probes were implanted into the dorsal subcutis of male Sprague-Dawley rats for 0,3,5 and 7 days. An internal standard, 2-deoxyglucose (2-DG), was passed through the microdialysis probe during in vivo studies. No significant differences in 2-DG extraction efficiency from the probe into the tissue site were observed in vivo among microdialysis probes implanted into the subcutaneous space of SpragueDawley rats for either three, five or seven days vs. probes implanted the day of sample collection. These results suggest that macrophage activation in vivo at implant sites is much lower than highly activated macrophages in vitro. It is important to note that these results do not rule out the potential for increased glucose metabolism at sensor implant sites.

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“…This suggests that the observed increase in the charge is not connected with the concentration of urea, but is related to the uric acid only. This increase in the charge in the presence of UA is a surprising result since most interfering compounds tend to foul, adhere to and block the surface of the electrode [39]. As a result, the signal is lost or lowered significantly.…”

Section: Selectivity Studies Of the Ppy-urs-scd Polymer Filmsmentioning

confidence: 97%

The development of a novel urea sensor using polypyrrole

Hamilton

Breslin

2014

Electrochimica Acta

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a b s t r a c tThe urease enzyme (Urs) was successfully incorporated into a polypyrrole film (PPy) in one simple electropolymerisation step. The films were formed using different dopant anions. The polypyrrole-urease-chloride film (PPy-Urs-Cl) was deposited from a simple chloride dopant, and the polypyrrole-urease-sulfonated-␤-cyclodextrin (PPy-Urs-SCD) was formed using a sulfonated-␤-cyclodextrin (SCD) dopant. The presence of the Urs within the polymer film was evident from the fibrous morphology, observed in the SEM micrographs, and the presence of nickel, arising from the active site of the urease enzyme. The sensing ability of the films and their enzyme-free counterparts (i.e., PPy-Cl and PPy-SCD) towards urea was investigated. The dopant anion plays an important role in the sensitivity of the polymer films towards urea. The PPy-Urs-SCD film has a superior sensitivity of 5.79 C M −1 compared to 0.76 C M −1 for the PPy-Urs-Cl polymer film. Furthermore, the negative groups on the SCD eliminate interference from common interfering compounds, such as ascorbic acid.

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Protein interactions with subcutaneously implanted biosensors

Cited by 109 publications

References 38 publications

Electrochemical detection of hydrogen peroxide on platinum-containing tetrahedral amorphous carbon sensors and evaluation of their biofouling properties

Electrochemical detection of hydrogen peroxide on platinum-containing tetrahedral amorphous carbon sensors and evaluation of their biofouling properties

Microdialysis Sampling Extraction Efficiency of 2-Deoxyglucose: Role of Macrophages in Vitro and in Vivo

The development of a novel urea sensor using polypyrrole

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