Protein mass transfer enhancement in the membrane filtration-cum-adsorption process

Xu, Yanke; Sirkar, Kamalesh K.

doi:10.1016/s0376-7388(03)00247-3

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…The apparent sieving coefficients have a characteristic minimum arising from the competing effects of its fluxdependent intrinsic membrane sieving coefficient and bulk concentration polarization (5,21,22). On the basis of classical membrane transport theory, the intrinsic membrane sieving coefficient decreases with increasing permeate flux, approaching its asymptotic value at high permeate flux as a result of the reduction in the contribution of solute diffusion relative to convection.…”

Section: Effect Of Permeate Flux On the Fractionation Of Hsa/higgmentioning

confidence: 99%

Untitled

2004

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The fractionation of the plasma proteins human serum albumin (HSA) and human immunoglobulins (HIgG) using the combination of two newly developed techniques, pulsed sample injection technique and carrier phase ultrafiltration (CPUF), is discussed in this paper. The effects of pH and ionic strength on the transmission of a single protein (i.e., either HSA or HIgG) through 100 and 300 kDa MWCO polyethersulfone (PES) membranes were quantified using the pulsed sample injection technique. The experimental results thus obtained suggested that it would be possible to fractionate these proteins by optimizing the solution pH and ionic strength. With 100 and 300 kDa PES membranes, effective separation of HSA and HIgG was achieved by CPUF using suitable conditions, i.e., pH 4.7 and low salt concentration. The fractionation of HSA and HIgG by "reverse selectivity" using 300 kDa membranes was also examined.

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Section: Effect Of Permeate Flux On the Fractionation Of Hsa/higgmentioning

confidence: 99%

Untitled

2004

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“…Consequently, the scale up of SEC is problematic due to larger capital costs for the additional equipment needed, greater risks for contamination and yield loss between unit operations, and inefficient steady state operation [60][61][62]…”

Section: Large-scale Protein Processing Technologiesmentioning

confidence: 99%

“…Additionally, when combined with semi-permeable membranes and membrane chromatography (i.e., membranes packed with chromatographic beads [62]), purifying multiple protein species from the same solution can also be accomplished [81].…”

Section: Defining the Customermentioning

confidence: 99%

A design for six sigma protein concentration process scale-up for black beans

Tom

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